Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)

Aqueous extracts, ethanolic precipitate and supernates of five brown seaweeds; Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata, were investigated for inhibition Nitric oxide (NO) production in lipopolysaccharide (LPS) induced macrophage RAW 264...

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Main Authors:	Hammed, Ademola Monsur, Simsek, Senay, Jaswir, Irwandi, Amid, Azura, Alam, Md. Zahangir
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	TP Chemical technology TP368 Food processing and manufacture TX341 Nutrition. Foods and food supply
Online Access:	http://irep.iium.edu.my/52176/ http://irep.iium.edu.my/52176/ http://irep.iium.edu.my/52176/1/Abic%202016.pdf

id	iium-52176
recordtype	eprints
spelling	iium-521762018-05-24T02:32:02Z http://irep.iium.edu.my/52176/ Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata) Hammed, Ademola Monsur Simsek, Senay Jaswir, Irwandi Amid, Azura Alam, Md. Zahangir TP Chemical technology TP368 Food processing and manufacture TX341 Nutrition. Foods and food supply Aqueous extracts, ethanolic precipitate and supernates of five brown seaweeds; Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata, were investigated for inhibition Nitric oxide (NO) production in lipopolysaccharide (LPS) induced macrophage RAW 264.7 cell line. All extracts contain high fucose and inhibit NO secretion in a dose dependent manner, precipitate of T. turbinata was highest in percentage yield 20% and dosage of 200g/ml down-regulated >80% NO secretion. Enzyme aided extraction was carried out on T. turbinata and released ethanolic precipitate was enhanced by the use of cellulase compared to vicozymes and amyloglucosidase. Eight processing conditions were screened with Plackett-Burman design and 3 significant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centered Central Composite Design in Response Surface Methods. Increase in yield was due to substrate ruptured by cellulase and the infrared spectra of sulfated polysaccharide confirmed presence of sulfated groups. Anion exchange chromatography separation gave three fractions: TtF1, TtF2 and TtF3. TtF3 has high percentage of fucose (60.920.09 %). TtF3 inhibited NO secretion in LPS induced macrophage with IC50 value of 103.76 g/ml. Secretion of PGE2, TNF-α and IL-8 were inhibited by TtF3. Cytotoxicity assay confirmed that TtF3 was nontoxic. The molecular weight TtF1, TtF2 and TtF3 are 223.5, 495.5 and 326.05 kDa, respectively. Proton NMR signals showed that the spectra of TtF2 and TtF3 possessed α-anomeric proton (5– 5.6 ppm), ring proton (3.4 – 4.4), O-acetyl group (2.2 ppm) and methyl proton (1 – 1.3 ppm). Thermal degradation pattern of TtF1 and TtF2 were similar compared to that of TtF3. The biological and physicochemical properties of the Sulfated polysaccahrides were related. The findings confirmed the potential of brown seaweed as a viable source of anti-inflammatory compounds. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52176/1/Abic%202016.pdf Hammed, Ademola Monsur and Simsek, Senay and Jaswir, Irwandi and Amid, Azura and Alam, Md. Zahangir (2016) Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata). In: Agricultural Bioscience International Conference (ABIC 2016), 18th-21st Sept. 2016, Fargo, North Dakota USA. (Unpublished) http://abic2016.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP368 Food processing and manufacture TX341 Nutrition. Foods and food supply
spellingShingle	TP Chemical technology TP368 Food processing and manufacture TX341 Nutrition. Foods and food supply Hammed, Ademola Monsur Simsek, Senay Jaswir, Irwandi Amid, Azura Alam, Md. Zahangir Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
description	Aqueous extracts, ethanolic precipitate and supernates of five brown seaweeds; Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata, were investigated for inhibition Nitric oxide (NO) production in lipopolysaccharide (LPS) induced macrophage RAW 264.7 cell line. All extracts contain high fucose and inhibit NO secretion in a dose dependent manner, precipitate of T. turbinata was highest in percentage yield 20% and dosage of 200g/ml down-regulated >80% NO secretion. Enzyme aided extraction was carried out on T. turbinata and released ethanolic precipitate was enhanced by the use of cellulase compared to vicozymes and amyloglucosidase. Eight processing conditions were screened with Plackett-Burman design and 3 significant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centered Central Composite Design in Response Surface Methods. Increase in yield was due to substrate ruptured by cellulase and the infrared spectra of sulfated polysaccharide confirmed presence of sulfated groups. Anion exchange chromatography separation gave three fractions: TtF1, TtF2 and TtF3. TtF3 has high percentage of fucose (60.920.09 %). TtF3 inhibited NO secretion in LPS induced macrophage with IC50 value of 103.76 g/ml. Secretion of PGE2, TNF-α and IL-8 were inhibited by TtF3. Cytotoxicity assay confirmed that TtF3 was nontoxic. The molecular weight TtF1, TtF2 and TtF3 are 223.5, 495.5 and 326.05 kDa, respectively. Proton NMR signals showed that the spectra of TtF2 and TtF3 possessed α-anomeric proton (5– 5.6 ppm), ring proton (3.4 – 4.4), O-acetyl group (2.2 ppm) and methyl proton (1 – 1.3 ppm). Thermal degradation pattern of TtF1 and TtF2 were similar compared to that of TtF3. The biological and physicochemical properties of the Sulfated polysaccahrides were related. The findings confirmed the potential of brown seaweed as a viable source of anti-inflammatory compounds.
format	Conference or Workshop Item
author	Hammed, Ademola Monsur Simsek, Senay Jaswir, Irwandi Amid, Azura Alam, Md. Zahangir
author_facet	Hammed, Ademola Monsur Simsek, Senay Jaswir, Irwandi Amid, Azura Alam, Md. Zahangir
author_sort	Hammed, Ademola Monsur
title	Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
title_short	Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
title_full	Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
title_fullStr	Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
title_full_unstemmed	Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)
title_sort	cellulase aided extraction and characteristics of sulfated polysaccharides from brown seaweed (turinaria turbinata)
publishDate	2016
url	http://irep.iium.edu.my/52176/ http://irep.iium.edu.my/52176/ http://irep.iium.edu.my/52176/1/Abic%202016.pdf
first_indexed	2023-09-18T21:13:57Z
last_indexed	2023-09-18T21:13:57Z
_version_	1777411437847642112

Cellulase aided extraction and characteristics of sulfated polysaccharides from Brown seaweed (Turinaria turbinata)

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