Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient

BACKGROUND: Spermatogonial stem cells (SSCs) are classifiedas a unique adult stem cells that have capability to propagate, differentiate, and transmit genetic information to the next generation. Studies on human SSCs may help resolve male infertility problems, especially in azoospermia patients. Th...

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Main Authors:	Abdul Wahab, Azantee Yazmie, Md Isa, Muhammad Lokman, Ramli, Roszaman
Format:	Article
Language:	English English English
Published:	Universiti Sains Malaysia 2016
Subjects:	RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/52198/ http://irep.iium.edu.my/52198/ http://irep.iium.edu.my/52198/1/spermatogonial%20Stem%20cell-Sr%20Azantee%202016.pdf http://irep.iium.edu.my/52198/7/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_SCOPUS.pdf http://irep.iium.edu.my/52198/8/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_WOS.pdf

id	iium-52198
recordtype	eprints
spelling	iium-521982016-12-21T00:31:12Z http://irep.iium.edu.my/52198/ Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman RG Gynecology and obstetrics BACKGROUND: Spermatogonial stem cells (SSCs) are classifiedas a unique adult stem cells that have capability to propagate, differentiate, and transmit genetic information to the next generation. Studies on human SSCs may help resolve male infertility problems, especially in azoospermia patients. Therefore, this study aims to propagate SSCs in-vitro with a presence of growth factor and detect SSC-specific protein cell surface markers. METHODS: The sample was derived from non-obstructive azoospermic (NOA) patient. The disassociation of SSCs was done using trypsin. Specific cultures in serum-free media with added basic fibroblast growth factor (bFGF) were developed to support self-renewal division. This undifferentiated protocol was performed for 49 days. Cells were analysed on days 1, 7, 14, 21, and 49. RESULTS: Human SSCs began to aggregate and form colonies after 14 to 21 days in specific culture. Then, the cells were successful expanded and remained stable for a duration of 49 days. Four specifics markers were identified using immunofluorescence in SSCs on day 49: ITGα6, ITGβ CD9, and GFRα1. CONCLUSION: This approach of using in vitro culture with additional growth factor is able to propagate SSCs from non-obstructive azoospermia patient via detection of protein cell surface markers. Universiti Sains Malaysia 2016-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/52198/1/spermatogonial%20Stem%20cell-Sr%20Azantee%202016.pdf application/pdf en http://irep.iium.edu.my/52198/7/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/52198/8/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_WOS.pdf Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Ramli, Roszaman (2016) Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient. Malaysian Journal of Medical Sciences, 23 (3). pp. 40-48. ISSN 1394-195X E-ISSN 2180-4303 http://journal.usm.my/journal/05mjms233_OA31.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RG Gynecology and obstetrics
spellingShingle	RG Gynecology and obstetrics Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
description	BACKGROUND: Spermatogonial stem cells (SSCs) are classifiedas a unique adult stem cells that have capability to propagate, differentiate, and transmit genetic information to the next generation. Studies on human SSCs may help resolve male infertility problems, especially in azoospermia patients. Therefore, this study aims to propagate SSCs in-vitro with a presence of growth factor and detect SSC-specific protein cell surface markers. METHODS: The sample was derived from non-obstructive azoospermic (NOA) patient. The disassociation of SSCs was done using trypsin. Specific cultures in serum-free media with added basic fibroblast growth factor (bFGF) were developed to support self-renewal division. This undifferentiated protocol was performed for 49 days. Cells were analysed on days 1, 7, 14, 21, and 49. RESULTS: Human SSCs began to aggregate and form colonies after 14 to 21 days in specific culture. Then, the cells were successful expanded and remained stable for a duration of 49 days. Four specifics markers were identified using immunofluorescence in SSCs on day 49: ITGα6, ITGβ CD9, and GFRα1. CONCLUSION: This approach of using in vitro culture with additional growth factor is able to propagate SSCs from non-obstructive azoospermia patient via detection of protein cell surface markers.
format	Article
author	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman
author_facet	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman
author_sort	Abdul Wahab, Azantee Yazmie
title	Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
title_short	Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
title_full	Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
title_fullStr	Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
title_full_unstemmed	Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
title_sort	spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient
publisher	Universiti Sains Malaysia
publishDate	2016
url	http://irep.iium.edu.my/52198/ http://irep.iium.edu.my/52198/ http://irep.iium.edu.my/52198/1/spermatogonial%20Stem%20cell-Sr%20Azantee%202016.pdf http://irep.iium.edu.my/52198/7/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_SCOPUS.pdf http://irep.iium.edu.my/52198/8/52198-Spermatogonial%20stem%20cells%20protein%20identification%20in_WOS.pdf
first_indexed	2023-09-18T21:13:59Z
last_indexed	2023-09-18T21:13:59Z
_version_	1777411439942696960

Spermatogonial stem cells protein identification in in vitro culture from non-obstructive azoospermia patient

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