Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics

Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C.purpureomaculatus. They were classified into 13 venom protein families consi...

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Main Authors:	Zainal Abidin, Syafiq Asnawi, Rajadurai, Pathmanathan, Chowdhury, Md Ezharul Hoque, Ahmad Rusmili, Muhamad Rusdi, Othman, Iekhsan, Naidu, Rakesh
Format:	Article
Language:	English English English
Published:	Multidisciplinary Digital Publishing Institute (MDPI) 2016
Subjects:	QL Zoology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/1/2016_Syafiq_Toxins_Proteomic%20of%20Viper%20Venom.pdf http://irep.iium.edu.my/52355/7/52355_Proteomic%20characterization_WOS_SCOPUS.pdf http://irep.iium.edu.my/52355/12/52355_Proteomic%20characterization%20and%20comparison_scopus.pdf

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recordtype	eprints
spelling	iium-523552017-10-21T11:02:42Z http://irep.iium.edu.my/52355/ Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics Zainal Abidin, Syafiq Asnawi Rajadurai, Pathmanathan Chowdhury, Md Ezharul Hoque Ahmad Rusmili, Muhamad Rusdi Othman, Iekhsan Naidu, Rakesh QL Zoology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C.purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in T. wagleri and C. purpureomaculatus venom were snake venom metalloproteinase, phospholipase A2, L-amino acid oxidase, serine proteases, 50-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in C. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to T. wagleri—it was not in C. purpureomaculatus venom. In contrast, cysteine-rich secretory protein,bradykinin-potentiating peptide, and C-type natriuretic peptide were present in C. purpureomaculatus venom. Composition of the venom proteome of T. wagleri and C. purpureomaculatus provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications. Multidisciplinary Digital Publishing Institute (MDPI) 2016-10-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/52355/1/2016_Syafiq_Toxins_Proteomic%20of%20Viper%20Venom.pdf application/pdf en http://irep.iium.edu.my/52355/7/52355_Proteomic%20characterization_WOS_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/52355/12/52355_Proteomic%20characterization%20and%20comparison_scopus.pdf Zainal Abidin, Syafiq Asnawi and Rajadurai, Pathmanathan and Chowdhury, Md Ezharul Hoque and Ahmad Rusmili, Muhamad Rusdi and Othman, Iekhsan and Naidu, Rakesh (2016) Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics. Toxins, 8 (10). 299-1-299-14. ISSN 2072-6651 http://www.mdpi.com/2072-6651/8/10/299/htm 10.3390/toxins8100299
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QL Zoology RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	QL Zoology RM Therapeutics. Pharmacology RS Pharmacy and materia medica Zainal Abidin, Syafiq Asnawi Rajadurai, Pathmanathan Chowdhury, Md Ezharul Hoque Ahmad Rusmili, Muhamad Rusdi Othman, Iekhsan Naidu, Rakesh Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
description	Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C.purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in T. wagleri and C. purpureomaculatus venom were snake venom metalloproteinase, phospholipase A2, L-amino acid oxidase, serine proteases, 50-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in C. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to T. wagleri—it was not in C. purpureomaculatus venom. In contrast, cysteine-rich secretory protein,bradykinin-potentiating peptide, and C-type natriuretic peptide were present in C. purpureomaculatus venom. Composition of the venom proteome of T. wagleri and C. purpureomaculatus provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.
format	Article
author	Zainal Abidin, Syafiq Asnawi Rajadurai, Pathmanathan Chowdhury, Md Ezharul Hoque Ahmad Rusmili, Muhamad Rusdi Othman, Iekhsan Naidu, Rakesh
author_facet	Zainal Abidin, Syafiq Asnawi Rajadurai, Pathmanathan Chowdhury, Md Ezharul Hoque Ahmad Rusmili, Muhamad Rusdi Othman, Iekhsan Naidu, Rakesh
author_sort	Zainal Abidin, Syafiq Asnawi
title	Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
title_short	Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
title_full	Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
title_fullStr	Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
title_full_unstemmed	Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics
title_sort	proteomic characterization and comparison of malaysian tropidolaemus wagleri and cryptelytrops purpureomaculatus venom using shotgun-proteomics
publisher	Multidisciplinary Digital Publishing Institute (MDPI)
publishDate	2016
url	http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/ http://irep.iium.edu.my/52355/1/2016_Syafiq_Toxins_Proteomic%20of%20Viper%20Venom.pdf http://irep.iium.edu.my/52355/7/52355_Proteomic%20characterization_WOS_SCOPUS.pdf http://irep.iium.edu.my/52355/12/52355_Proteomic%20characterization%20and%20comparison_scopus.pdf
first_indexed	2023-09-18T21:14:11Z
last_indexed	2023-09-18T21:14:11Z
_version_	1777411451734982656

Proteomic characterization and comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus venom using shotgun-proteomics

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