Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis

Introduction: Protein profiling of harmful algae is an ongoing study where the latest analysis was conducted on A. minutum in 2015. It is a fundamental study where the protein expression of targeted species can be used to understand the biochemical pathway of selected proteins. Alexandrium spp. in...

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Main Authors:	Hamdan, Nurul Ashima, Mohammad Noor, Normawaty, Abd Hamid, Shafida, Md Zin, Noor Hasniza, Muhamad Bunnori, Noraslinda
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	ND1288 Special subjects. Human figure. Portraits. Lanscape painting. Marine painting. Animal. Birds. Sports.Flowers. Fruits etc.
Online Access:	http://irep.iium.edu.my/52510/ http://irep.iium.edu.my/52510/40/52510.pdf

id	iium-52510
recordtype	eprints
spelling	iium-525102018-09-25T07:27:47Z http://irep.iium.edu.my/52510/ Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis Hamdan, Nurul Ashima Mohammad Noor, Normawaty Abd Hamid, Shafida Md Zin, Noor Hasniza Muhamad Bunnori, Noraslinda ND1288 Special subjects. Human figure. Portraits. Lanscape painting. Marine painting. Animal. Birds. Sports.Flowers. Fruits etc. Introduction: Protein profiling of harmful algae is an ongoing study where the latest analysis was conducted on A. minutum in 2015. It is a fundamental study where the protein expression of targeted species can be used to understand the biochemical pathway of selected proteins. Alexandrium spp. in Malaysia has the potential to cause massive blooming that brings harm to the aquatic ecosystem. Methods: In this experiment three methods of cell lysis were tested against A. leei isolated from Malaysian waters. Axenic culture of the sample was established in enriched seawater media (ESDK) with 12 hours of light and 12 hours of dark conditions. The sample was extracted during exponential phase (day 18) where the same amount of cells was collected via centrifugation. Same buffer was used for each technique of cell lysis in order to get the best protein profiles in terms of band or spot intensity and number. The cells of A. leei were lysed using sonication in ice-cold water bath, sonication with probe and freeze-thawing followed by sonication in iced bath. Conclusion: Nevertheless it can be concluded from the qualitative analysis using SDS-PAGE and 2D gel electrophoresis that freeze thawing mode of cell lysis produced excellent result among others as the protein spots produced were precise with less streaking observed. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52510/40/52510.pdf Hamdan, Nurul Ashima and Mohammad Noor, Normawaty and Abd Hamid, Shafida and Md Zin, Noor Hasniza and Muhamad Bunnori, Noraslinda (2016) Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis. In: Medical Research Symposium and Pacific Partnership in Conjunction with Kuantan Research Day 2016, 4th August 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	ND1288 Special subjects. Human figure. Portraits. Lanscape painting. Marine painting. Animal. Birds. Sports.Flowers. Fruits etc.
spellingShingle	ND1288 Special subjects. Human figure. Portraits. Lanscape painting. Marine painting. Animal. Birds. Sports.Flowers. Fruits etc. Hamdan, Nurul Ashima Mohammad Noor, Normawaty Abd Hamid, Shafida Md Zin, Noor Hasniza Muhamad Bunnori, Noraslinda Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
description	Introduction: Protein profiling of harmful algae is an ongoing study where the latest analysis was conducted on A. minutum in 2015. It is a fundamental study where the protein expression of targeted species can be used to understand the biochemical pathway of selected proteins. Alexandrium spp. in Malaysia has the potential to cause massive blooming that brings harm to the aquatic ecosystem. Methods: In this experiment three methods of cell lysis were tested against A. leei isolated from Malaysian waters. Axenic culture of the sample was established in enriched seawater media (ESDK) with 12 hours of light and 12 hours of dark conditions. The sample was extracted during exponential phase (day 18) where the same amount of cells was collected via centrifugation. Same buffer was used for each technique of cell lysis in order to get the best protein profiles in terms of band or spot intensity and number. The cells of A. leei were lysed using sonication in ice-cold water bath, sonication with probe and freeze-thawing followed by sonication in iced bath. Conclusion: Nevertheless it can be concluded from the qualitative analysis using SDS-PAGE and 2D gel electrophoresis that freeze thawing mode of cell lysis produced excellent result among others as the protein spots produced were precise with less streaking observed.
format	Conference or Workshop Item
author	Hamdan, Nurul Ashima Mohammad Noor, Normawaty Abd Hamid, Shafida Md Zin, Noor Hasniza Muhamad Bunnori, Noraslinda
author_facet	Hamdan, Nurul Ashima Mohammad Noor, Normawaty Abd Hamid, Shafida Md Zin, Noor Hasniza Muhamad Bunnori, Noraslinda
author_sort	Hamdan, Nurul Ashima
title	Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
title_short	Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
title_full	Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
title_fullStr	Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
title_full_unstemmed	Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis
title_sort	differential methods of cell lysis for protein profiling of alexandrium sp. using 2d gel electrophoresis
publishDate	2016
url	http://irep.iium.edu.my/52510/ http://irep.iium.edu.my/52510/40/52510.pdf
first_indexed	2023-09-18T21:14:23Z
last_indexed	2023-09-18T21:14:23Z
_version_	1777411464942845952

Differential methods of cell lysis for protein profiling of Alexandrium sp. using 2D gel electrophoresis

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