Fluorescence multispectral imaging-based diagnostic system for atherosclerosis

Background Composition of atherosclerotic arterial walls is rich in lipids such as cholesterol, unlike normal arterial walls. In this study, we aimed to utilize this difference to diagnose atherosclerosis via multispectral fluorescence imaging, which allows for identification of fluorescence origin...

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Main Authors:	Ho, Cassandra Su Lyn, Horiuchi, Toshikatsu, Taniguchi, Hiroak, Umetsu, Araya, Hagisawa, Kohsuke, Iwaya, Keiichi, Nakai, Kanji, Azmi, Amalina, Zulaziz, Natasha, Azhim, Azran, Shinomiya, Nariyoshi, Morimoto, Yuji
Format:	Article
Language:	English English English
Published:	BioMed Central 2016
Subjects:	RB Pathology RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/1/art-3A10.1186-2Fs12938-016-0220-z.pdf http://irep.iium.edu.my/52526/7/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_SCOPUS.pdf http://irep.iium.edu.my/52526/8/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_WOS.pdf

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spelling	iium-525262016-12-21T07:43:31Z http://irep.iium.edu.my/52526/ Fluorescence multispectral imaging-based diagnostic system for atherosclerosis Ho, Cassandra Su Lyn Horiuchi, Toshikatsu Taniguchi, Hiroak Umetsu, Araya Hagisawa, Kohsuke Iwaya, Keiichi Nakai, Kanji Azmi, Amalina Zulaziz, Natasha Azhim, Azran Shinomiya, Nariyoshi Morimoto, Yuji RB Pathology RM Therapeutics. Pharmacology Background Composition of atherosclerotic arterial walls is rich in lipids such as cholesterol, unlike normal arterial walls. In this study, we aimed to utilize this difference to diagnose atherosclerosis via multispectral fluorescence imaging, which allows for identification of fluorescence originating from the substance in the arterial wall. Methods The inner surface of extracted arteries (rabbit abdominal aorta, human coronary artery) was illuminated by 405 nm excitation light and multispectral fluorescence images were obtained. Pathological examination of human coronary artery samples were carried out and thickness of arteries were calculated by measuring combined media and intima thickness. Results The fluorescence spectra in atherosclerotic sites were different from those in normal sites. Multiple regions of interest (ROI) were selected within each sample and a ratio between two fluorescence intensity differences (where each intensity difference is calculated between an identifier wavelength and a base wavelength) from each ROI was determined, allowing for discrimination of atherosclerotic sites. Fluorescence intensity and thickness of artery were found to be significantly correlated. Conclusions These results indicate that multispectral fluorescence imaging provides qualitative and quantitative evaluations of atherosclerosis and is therefore a viable method of diagnosing the disease. BioMed Central 2016-08-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/52526/1/art-3A10.1186-2Fs12938-016-0220-z.pdf application/pdf en http://irep.iium.edu.my/52526/7/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/52526/8/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_WOS.pdf Ho, Cassandra Su Lyn and Horiuchi, Toshikatsu and Taniguchi, Hiroak and Umetsu, Araya and Hagisawa, Kohsuke and Iwaya, Keiichi and Nakai, Kanji and Azmi, Amalina and Zulaziz, Natasha and Azhim, Azran and Shinomiya, Nariyoshi and Morimoto, Yuji (2016) Fluorescence multispectral imaging-based diagnostic system for atherosclerosis. BioMedical Engineering OnLine, 15 (98). pp. 1-10. ISSN 1475-925X https://biomedical-engineering-online.biomedcentral.com/articles/10.1186/s12938-016-0220-z 10.1186/s12938-016-0220-z
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RB Pathology RM Therapeutics. Pharmacology
spellingShingle	RB Pathology RM Therapeutics. Pharmacology Ho, Cassandra Su Lyn Horiuchi, Toshikatsu Taniguchi, Hiroak Umetsu, Araya Hagisawa, Kohsuke Iwaya, Keiichi Nakai, Kanji Azmi, Amalina Zulaziz, Natasha Azhim, Azran Shinomiya, Nariyoshi Morimoto, Yuji Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
description	Background Composition of atherosclerotic arterial walls is rich in lipids such as cholesterol, unlike normal arterial walls. In this study, we aimed to utilize this difference to diagnose atherosclerosis via multispectral fluorescence imaging, which allows for identification of fluorescence originating from the substance in the arterial wall. Methods The inner surface of extracted arteries (rabbit abdominal aorta, human coronary artery) was illuminated by 405 nm excitation light and multispectral fluorescence images were obtained. Pathological examination of human coronary artery samples were carried out and thickness of arteries were calculated by measuring combined media and intima thickness. Results The fluorescence spectra in atherosclerotic sites were different from those in normal sites. Multiple regions of interest (ROI) were selected within each sample and a ratio between two fluorescence intensity differences (where each intensity difference is calculated between an identifier wavelength and a base wavelength) from each ROI was determined, allowing for discrimination of atherosclerotic sites. Fluorescence intensity and thickness of artery were found to be significantly correlated. Conclusions These results indicate that multispectral fluorescence imaging provides qualitative and quantitative evaluations of atherosclerosis and is therefore a viable method of diagnosing the disease.
format	Article
author	Ho, Cassandra Su Lyn Horiuchi, Toshikatsu Taniguchi, Hiroak Umetsu, Araya Hagisawa, Kohsuke Iwaya, Keiichi Nakai, Kanji Azmi, Amalina Zulaziz, Natasha Azhim, Azran Shinomiya, Nariyoshi Morimoto, Yuji
author_facet	Ho, Cassandra Su Lyn Horiuchi, Toshikatsu Taniguchi, Hiroak Umetsu, Araya Hagisawa, Kohsuke Iwaya, Keiichi Nakai, Kanji Azmi, Amalina Zulaziz, Natasha Azhim, Azran Shinomiya, Nariyoshi Morimoto, Yuji
author_sort	Ho, Cassandra Su Lyn
title	Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
title_short	Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
title_full	Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
title_fullStr	Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
title_full_unstemmed	Fluorescence multispectral imaging-based diagnostic system for atherosclerosis
title_sort	fluorescence multispectral imaging-based diagnostic system for atherosclerosis
publisher	BioMed Central
publishDate	2016
url	http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/ http://irep.iium.edu.my/52526/1/art-3A10.1186-2Fs12938-016-0220-z.pdf http://irep.iium.edu.my/52526/7/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_SCOPUS.pdf http://irep.iium.edu.my/52526/8/52526-Fluorescence%20multispectral%20imaging-based%20diagnostic%20system%20for%20atherosclerosis_WOS.pdf
first_indexed	2023-09-18T21:14:24Z
last_indexed	2023-09-18T21:14:24Z
_version_	1777411466103619584

Fluorescence multispectral imaging-based diagnostic system for atherosclerosis

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