Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy

Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nucl...

Full description

Bibliographic Details
Main Authors:	Ab. Hadi, Hazrina, Azmar, Hawa Mas
Format:	Conference or Workshop Item
Language:	English English English
Published:	2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/52614/ http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf http://irep.iium.edu.my/52614/3/Cover%20page.pdf

id	iium-52614
recordtype	eprints
spelling	iium-526142017-03-10T17:37:24Z http://irep.iium.edu.my/52614/ Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy Ab. Hadi, Hazrina Azmar, Hawa Mas RS Pharmacy and materia medica Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nuclei that are magnetically separated as well as the nature of the environment of the nuclei. Aims: In this study, NMR is used to identify the isolated hydroxychavicol. The isolated hydroxychavicol was dissolved in deuterated methanol. Methods: The sample is analyzed for 1D NMR (1H and 13C) spectra using 500 MHz NMR (Bruker; Avance III Ultrashield Plus; Switzerland) located in Universiti Malaysia Pahang. The target directed isolation of hydroxychavicol was performed by comparing bioautographic profile of hydroxychavicol-rich fraction to that of hydroxychavicol standard. Hydroxychavicol is a known compound with molecular formula of C9H10O2 and molecular weight of 150.174 g/mol. Results and discussion: 1H and 13C NMR elucidated the structure of the isolated hydroxychavicol. The 1H NMR spectrum furnished 10 protons at chemical shifts of δH 3.21, 4.90, 4.98, 5.9, 6.49, 6.61, and 6.67 ppm. Based on the spectrum, a broad spectrum was displayed meaning there is a hydroxyl group in the structure. The 13C NMR spectrum displayed the nine carbons at chemical shifts δC 40.64, 115.33, 116.31, 116.76, 120.87, 132.99, 139.57, 144.46, and 146.17 ppm. Conclusion: The isolated hydroxychavicol was successfully being authenticated by NMR. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf application/pdf en http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf application/pdf en http://irep.iium.edu.my/52614/3/Cover%20page.pdf Ab. Hadi, Hazrina and Azmar, Hawa Mas (2016) Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy. In: Pharma BioAsia Conference 2016, 28-29 Sep 2016, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Ab. Hadi, Hazrina Azmar, Hawa Mas Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
description	Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nuclei that are magnetically separated as well as the nature of the environment of the nuclei. Aims: In this study, NMR is used to identify the isolated hydroxychavicol. The isolated hydroxychavicol was dissolved in deuterated methanol. Methods: The sample is analyzed for 1D NMR (1H and 13C) spectra using 500 MHz NMR (Bruker; Avance III Ultrashield Plus; Switzerland) located in Universiti Malaysia Pahang. The target directed isolation of hydroxychavicol was performed by comparing bioautographic profile of hydroxychavicol-rich fraction to that of hydroxychavicol standard. Hydroxychavicol is a known compound with molecular formula of C9H10O2 and molecular weight of 150.174 g/mol. Results and discussion: 1H and 13C NMR elucidated the structure of the isolated hydroxychavicol. The 1H NMR spectrum furnished 10 protons at chemical shifts of δH 3.21, 4.90, 4.98, 5.9, 6.49, 6.61, and 6.67 ppm. Based on the spectrum, a broad spectrum was displayed meaning there is a hydroxyl group in the structure. The 13C NMR spectrum displayed the nine carbons at chemical shifts δC 40.64, 115.33, 116.31, 116.76, 120.87, 132.99, 139.57, 144.46, and 146.17 ppm. Conclusion: The isolated hydroxychavicol was successfully being authenticated by NMR.
format	Conference or Workshop Item
author	Ab. Hadi, Hazrina Azmar, Hawa Mas
author_facet	Ab. Hadi, Hazrina Azmar, Hawa Mas
author_sort	Ab. Hadi, Hazrina
title	Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
title_short	Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
title_full	Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
title_fullStr	Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
title_full_unstemmed	Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
title_sort	authentication of isolated hydroxychavicol from p. betle linn. leaves using nuclear magnetic resonance spectroscopy
publishDate	2016
url	http://irep.iium.edu.my/52614/ http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf http://irep.iium.edu.my/52614/3/Cover%20page.pdf
first_indexed	2023-09-18T21:14:30Z
last_indexed	2023-09-18T21:14:30Z
_version_	1777411472390881280

Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy

Similar Items