Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12

Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acid...

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Main Authors:	Alias, Norsyuhada, Raja Abd. Rahman, Raja Noor Zaliha
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/53236/ http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf

id	iium-53236
recordtype	eprints
spelling	iium-532362016-12-01T02:10:49Z http://irep.iium.edu.my/53236/ Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12 Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha QR Microbiology Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acids. Homology search by BLAST web server has showed the PI12 protease sequence shared a significant homology to the subtilisin protease family from fungus but no similarity with other psychrophilic protease. The protease sequence was deposited into the GenBank Database with the accession no. CAQ76821. The gene encoding mature PI12 protease was cloned into Pichia pastoris expression vector, pPIC9, and positioned under the induction of methanol-alcohol oxidase (AOX) promoter. The recombinant PI12 protease was efficiently secreted into the culture medium driven by the Saccharomyces cerevisiae α-factor signal sequence. The highest protease production was obtained from P. pastoris GS115 host (GpPro2) with 20.3 U/mL activity at 15ºC after 3 days of induction time. The expressed protein was detected by SDS-PAGE and activity staining with a molecular weight of 99.3 kDa. 2014-02-10 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf Alias, Norsyuhada and Raja Abd. Rahman, Raja Noor Zaliha (2014) Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12. In: 3rd Biotechnology World Congress, 10-12 February 2014, Dubai, UAE. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
description	Leucosporidium antarcticum strain PI12 was isolated from Casey Station, Antarctica. It was shown to be protease-producer. Full-length cDNA of PI12 protease gene was amplified by rapid amplification of cDNA ends (RACE) strategy with an open reading frame (ORF) of 2892 bp that coded for 963 amino acids. Homology search by BLAST web server has showed the PI12 protease sequence shared a significant homology to the subtilisin protease family from fungus but no similarity with other psychrophilic protease. The protease sequence was deposited into the GenBank Database with the accession no. CAQ76821. The gene encoding mature PI12 protease was cloned into Pichia pastoris expression vector, pPIC9, and positioned under the induction of methanol-alcohol oxidase (AOX) promoter. The recombinant PI12 protease was efficiently secreted into the culture medium driven by the Saccharomyces cerevisiae α-factor signal sequence. The highest protease production was obtained from P. pastoris GS115 host (GpPro2) with 20.3 U/mL activity at 15ºC after 3 days of induction time. The expressed protein was detected by SDS-PAGE and activity staining with a molecular weight of 99.3 kDa.
format	Conference or Workshop Item
author	Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha
author_facet	Alias, Norsyuhada Raja Abd. Rahman, Raja Noor Zaliha
author_sort	Alias, Norsyuhada
title	Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
title_short	Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
title_full	Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
title_fullStr	Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
title_full_unstemmed	Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12
title_sort	cold-adapted serine protease from antarctic yeast leucosporidium antarcticum strain pi12
publishDate	2014
url	http://irep.iium.edu.my/53236/ http://irep.iium.edu.my/53236/1/3rd-BWC-Abstract-Book_53236_11216.pdf
first_indexed	2023-09-18T21:15:22Z
last_indexed	2023-09-18T21:15:22Z
_version_	1777411526594920448

Cold-adapted serine protease from Antarctic yeast Leucosporidium antarcticum strain PI12

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