Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics

Neptunia oleracea is a plant consumed as vegetable and used as a traditional herb to treat several ailments. This study evaluated metabolite variations among N. oleracea leaf and stem subjected to air drying (AD), freeze drying (FD) and oven drying (OD) using proton nuclear magnetic resonance (1H NM...

Full description

Bibliographic Details
Main Authors:	Lee, Soo Yee, Abas, Faridah, Khatib, Alfi, Ismail, Intan Safinar, Shaari, Khozirah, Zawawi, Norhasnida
Format:	Article
Language:	English English English
Published:	2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/53533/ http://irep.iium.edu.my/53533/ http://irep.iium.edu.my/53533/1/lee2016.pdf http://irep.iium.edu.my/53533/7/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf http://irep.iium.edu.my/53533/12/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf

id	iium-53533
recordtype	eprints
spelling	iium-535332017-10-12T12:03:13Z http://irep.iium.edu.my/53533/ Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics Lee, Soo Yee Abas, Faridah Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Zawawi, Norhasnida QD Chemistry Neptunia oleracea is a plant consumed as vegetable and used as a traditional herb to treat several ailments. This study evaluated metabolite variations among N. oleracea leaf and stem subjected to air drying (AD), freeze drying (FD) and oven drying (OD) using proton nuclear magnetic resonance (1H NMR) based metabolomics. The correlation was also studied for the metabolite content with total phenolic content (TPC), DPPH free radical scavenging and α-glucosidase inhibitory activities. A total of 18 metabolites were identified from N. oleracea extracts, including 10 primary metabolites, 5 flavonoids and 3 phenolic acids using NMR. Ultra-high performance liquid chromatography tandem mass spectrometry analysis (UHPLC-MS/MS) confirmed the presence of the secondary metabolites and revealed the flavonoid derivatives present. All the identified phenolics are first reported from this plant. Multivariate data analysis (MVDA) showed strong correlation between the metabolites with the antioxidant and α-glucosidase inhibitory activities of FD N. oleracea leaves. The compounds suggested to be responsible for the high activity of FD leaves include vitexin-2-O-rhamnoside, catechin, caffeic acid, gallic acid and derivatives of quercetin, kaempferol and myricetin. This study demonstrates that FD N. oleracea leaves are a potential natural source for antioxidant and α-glucosidase inhibitors. 2016-03-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53533/1/lee2016.pdf application/pdf en http://irep.iium.edu.my/53533/7/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf application/pdf en http://irep.iium.edu.my/53533/12/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf Lee, Soo Yee and Abas, Faridah and Khatib, Alfi and Ismail, Intan Safinar and Shaari, Khozirah and Zawawi, Norhasnida (2016) Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics. Phytocehmistry Letters, 16. pp. 23-33. ISSN 1874-3900 10.1016/j.phytol.2016.02.014
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QD Chemistry
spellingShingle	QD Chemistry Lee, Soo Yee Abas, Faridah Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Zawawi, Norhasnida Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
description	Neptunia oleracea is a plant consumed as vegetable and used as a traditional herb to treat several ailments. This study evaluated metabolite variations among N. oleracea leaf and stem subjected to air drying (AD), freeze drying (FD) and oven drying (OD) using proton nuclear magnetic resonance (1H NMR) based metabolomics. The correlation was also studied for the metabolite content with total phenolic content (TPC), DPPH free radical scavenging and α-glucosidase inhibitory activities. A total of 18 metabolites were identified from N. oleracea extracts, including 10 primary metabolites, 5 flavonoids and 3 phenolic acids using NMR. Ultra-high performance liquid chromatography tandem mass spectrometry analysis (UHPLC-MS/MS) confirmed the presence of the secondary metabolites and revealed the flavonoid derivatives present. All the identified phenolics are first reported from this plant. Multivariate data analysis (MVDA) showed strong correlation between the metabolites with the antioxidant and α-glucosidase inhibitory activities of FD N. oleracea leaves. The compounds suggested to be responsible for the high activity of FD leaves include vitexin-2-O-rhamnoside, catechin, caffeic acid, gallic acid and derivatives of quercetin, kaempferol and myricetin. This study demonstrates that FD N. oleracea leaves are a potential natural source for antioxidant and α-glucosidase inhibitors.
format	Article
author	Lee, Soo Yee Abas, Faridah Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Zawawi, Norhasnida
author_facet	Lee, Soo Yee Abas, Faridah Khatib, Alfi Ismail, Intan Safinar Shaari, Khozirah Zawawi, Norhasnida
author_sort	Lee, Soo Yee
title	Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
title_short	Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
title_full	Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
title_fullStr	Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
title_full_unstemmed	Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics
title_sort	metabolite profiling of neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1h nmr-based metabolomics
publishDate	2016
url	http://irep.iium.edu.my/53533/ http://irep.iium.edu.my/53533/ http://irep.iium.edu.my/53533/1/lee2016.pdf http://irep.iium.edu.my/53533/7/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf http://irep.iium.edu.my/53533/12/53533_Metabolite%20profiling%20of%20Neptunia%20oleracea%20and%20correlation%20with%20antioxidant_wos.pdf
first_indexed	2023-09-18T21:15:43Z
last_indexed	2023-09-18T21:15:43Z
_version_	1777411548260597760

Metabolite profiling of Neptunia oleracea and correlation with antioxidant and α-glucosidase inhibitory activities using 1H NMR-based metabolomics

Similar Items