Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent

Oral biofilms comprise of extracellular polysaccharides and polymicrobial microorgan- isms. The objective of this study was to determine the effect of polymicrobial interac- tions of Candida albicans, Actinomyces naeslundii, and Streptococcus mutans on biofilm formation with the hypotheses that biof...

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Main Authors:	Arzmi, Mohd Hafiz, Alnuaimi, Ali D., Dashper, Stuart, Cirillo, Nicola, Reynolds, Eric, McCullough, Michael
Format:	Article
Language:	English English English
Published:	Oxford University Press 2016
Subjects:	QR Microbiology RK Dentistry
Online Access:	http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/2/Polymicrobial%20biofilm%20formation%20by%20Candida%20albicans%2C%20Actinomyces%20naeslundii%2C%20and%20Streptococcus%20mutans%20is%20Candida%20albicans%20strain%20and%20medium%20dependent.pdf http://irep.iium.edu.my/53623/3/53623_Polymicrobial%20biofilm%20formation_SCOPUS.pdf http://irep.iium.edu.my/53623/4/53623_Polymicrobial%20biofilm%20formation_WOS.pdf

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recordtype	eprints
spelling	iium-536232017-10-12T10:22:27Z http://irep.iium.edu.my/53623/ Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent Arzmi, Mohd Hafiz Alnuaimi, Ali D. Dashper, Stuart Cirillo, Nicola Reynolds, Eric McCullough, Michael QR Microbiology RK Dentistry Oral biofilms comprise of extracellular polysaccharides and polymicrobial microorgan- isms. The objective of this study was to determine the effect of polymicrobial interac- tions of Candida albicans, Actinomyces naeslundii, and Streptococcus mutans on biofilm formation with the hypotheses that biofilm biomass and metabolic activity are both C. albicans strain and growth medium dependent. To study monospecific biofilms, C. al- bicans, A. naeslundii, and S. mutans were inoculated into artificial saliva medium (ASM) and RPMI-1640 in separate vials, whereas to study polymicrobial biofilm formation, the inoculum containing microorganisms was prepared in the same vial prior inoculation into a 96-well plate followed by 72 hours incubation. Finally, biofilm biomass and metabolic activity were measured using crystal violet and XTT assays, respectively. Our results showed variability of monospecies and polymicrobial biofilm biomass between C. al- bicans strains and growth medium. Based on cut-offs, out of 32, seven RPMI-grown biofilms had high biofilm biomass (HBB), whereas, in ASM-grown biofilms, 14 out of 32 were HBB. Of the 32 biofilms grown in RPMI-1640, 21 were high metabolic activity (HMA), whereas in ASM, there was no biofilm had HMA. Significant differences were observed between ASM and RPMI-grown biofilms with respect to metabolic activity (P < .01). In conclusion, biofilm biomass and metabolic activity were both C. albicans strain and growth medium dependent. Oxford University Press 2016-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53623/2/Polymicrobial%20biofilm%20formation%20by%20Candida%20albicans%2C%20Actinomyces%20naeslundii%2C%20and%20Streptococcus%20mutans%20is%20Candida%20albicans%20strain%20and%20medium%20dependent.pdf application/pdf en http://irep.iium.edu.my/53623/3/53623_Polymicrobial%20biofilm%20formation_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/53623/4/53623_Polymicrobial%20biofilm%20formation_WOS.pdf Arzmi, Mohd Hafiz and Alnuaimi, Ali D. and Dashper, Stuart and Cirillo, Nicola and Reynolds, Eric and McCullough, Michael (2016) Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent. Medical Mycology, 58 (8). pp. 856-864. ISSN 1369-3786 E-ISSN 1460-2709 http://mmy.oxfordjournals.org/content/54/8/856.long 10.1093/mmy/myw042
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QR Microbiology RK Dentistry
spellingShingle	QR Microbiology RK Dentistry Arzmi, Mohd Hafiz Alnuaimi, Ali D. Dashper, Stuart Cirillo, Nicola Reynolds, Eric McCullough, Michael Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
description	Oral biofilms comprise of extracellular polysaccharides and polymicrobial microorgan- isms. The objective of this study was to determine the effect of polymicrobial interac- tions of Candida albicans, Actinomyces naeslundii, and Streptococcus mutans on biofilm formation with the hypotheses that biofilm biomass and metabolic activity are both C. albicans strain and growth medium dependent. To study monospecific biofilms, C. al- bicans, A. naeslundii, and S. mutans were inoculated into artificial saliva medium (ASM) and RPMI-1640 in separate vials, whereas to study polymicrobial biofilm formation, the inoculum containing microorganisms was prepared in the same vial prior inoculation into a 96-well plate followed by 72 hours incubation. Finally, biofilm biomass and metabolic activity were measured using crystal violet and XTT assays, respectively. Our results showed variability of monospecies and polymicrobial biofilm biomass between C. al- bicans strains and growth medium. Based on cut-offs, out of 32, seven RPMI-grown biofilms had high biofilm biomass (HBB), whereas, in ASM-grown biofilms, 14 out of 32 were HBB. Of the 32 biofilms grown in RPMI-1640, 21 were high metabolic activity (HMA), whereas in ASM, there was no biofilm had HMA. Significant differences were observed between ASM and RPMI-grown biofilms with respect to metabolic activity (P < .01). In conclusion, biofilm biomass and metabolic activity were both C. albicans strain and growth medium dependent.
format	Article
author	Arzmi, Mohd Hafiz Alnuaimi, Ali D. Dashper, Stuart Cirillo, Nicola Reynolds, Eric McCullough, Michael
author_facet	Arzmi, Mohd Hafiz Alnuaimi, Ali D. Dashper, Stuart Cirillo, Nicola Reynolds, Eric McCullough, Michael
author_sort	Arzmi, Mohd Hafiz
title	Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
title_short	Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
title_full	Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
title_fullStr	Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
title_full_unstemmed	Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent
title_sort	polymicrobial biofilm formation by candida albicans, actinomyces naeslundii, and streptococcus mutans is candida albicans strain and medium dependent
publisher	Oxford University Press
publishDate	2016
url	http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/ http://irep.iium.edu.my/53623/2/Polymicrobial%20biofilm%20formation%20by%20Candida%20albicans%2C%20Actinomyces%20naeslundii%2C%20and%20Streptococcus%20mutans%20is%20Candida%20albicans%20strain%20and%20medium%20dependent.pdf http://irep.iium.edu.my/53623/3/53623_Polymicrobial%20biofilm%20formation_SCOPUS.pdf http://irep.iium.edu.my/53623/4/53623_Polymicrobial%20biofilm%20formation_WOS.pdf
first_indexed	2023-09-18T21:15:51Z
last_indexed	2023-09-18T21:15:51Z
_version_	1777411556863115264

Polymicrobial biofilm formation by Candida albicans, Actinomyces naeslundii, and Streptococcus mutans is Candida albicans strain and medium dependent

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