Comparative structural analysis of fruit and stem bromelain from Ananas comosus

Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide...

Full description

Bibliographic Details
Main Authors: Ramli, Aizi Nor Mazila, Abdul Manas, Nor Hasmaliana, Abdul Hamid, Azzmer Azzar, Abd. Hamid, Hazrulrizawati, Md. Illias, Rosli
Format: Article
Language:English
English
English
Published: Elsevier Ireland Ltd 2018
Subjects:
Online Access:http://irep.iium.edu.my/53935/
http://irep.iium.edu.my/53935/
http://irep.iium.edu.my/53935/
http://irep.iium.edu.my/53935/1/53935_Comparative%20structural%20analysis.pdf
http://irep.iium.edu.my/53935/2/53935_Comparative%20structural%20analysis_SCOPUS.pdf
http://irep.iium.edu.my/53935/13/53935_Comparative%20structural%20analysis%20of%20fruit%20and%20stem%20bromelain%20from%20Ananas%20comosus_WoS.pdf
Description
Summary:Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application.