Effects of ionizing radiation on chondrocytes growth: work in progress

Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three...

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Main Authors:	Mat Nawi, Nur Farhana, Sha'ban, Munirah, Zainuddin, Zainul Ibrahim
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/53947/ http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf

id	iium-53947
recordtype	eprints
spelling	iium-539472016-12-26T04:38:28Z http://irep.iium.edu.my/53947/ Effects of ionizing radiation on chondrocytes growth: work in progress Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim Q Science (General) Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage. Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control. Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes. 2016-11-17 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf application/pdf en http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf Mat Nawi, Nur Farhana and Sha'ban, Munirah and Zainuddin, Zainul Ibrahim (2016) Effects of ionizing radiation on chondrocytes growth: work in progress. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17 - 18 Nov 2016, Penang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim Effects of ionizing radiation on chondrocytes growth: work in progress
description	Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage. Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control. Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes.
format	Conference or Workshop Item
author	Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim
author_facet	Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim
author_sort	Mat Nawi, Nur Farhana
title	Effects of ionizing radiation on chondrocytes growth: work in progress
title_short	Effects of ionizing radiation on chondrocytes growth: work in progress
title_full	Effects of ionizing radiation on chondrocytes growth: work in progress
title_fullStr	Effects of ionizing radiation on chondrocytes growth: work in progress
title_full_unstemmed	Effects of ionizing radiation on chondrocytes growth: work in progress
title_sort	effects of ionizing radiation on chondrocytes growth: work in progress
publishDate	2016
url	http://irep.iium.edu.my/53947/ http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf
first_indexed	2023-09-18T21:16:19Z
last_indexed	2023-09-18T21:16:19Z
_version_	1777411586538864640

Effects of ionizing radiation on chondrocytes growth: work in progress

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