Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana

In the Erwinia amylovora genome, the hrp gene cluster containing the dspA/E/EB/F operon plays a crucial role in mediating the pathogenicity and the hypersensitive response (HR) in the host plant. The role of the dspA/E gene derived from E. amylovora was investigated by monitoring the expression o...

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Main Authors:	Aksoy, Hasan Murad, Kaya, Yilmaz, Tengku Abdul Hamid, Tengku Haziyamin
Format:	Article
Language:	English English
Published:	Taylor and Francis Ltd. 2017
Subjects:	QH Natural history QH301 Biology
Online Access:	http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/1/Expression%20of%20the%20dspA%20E%20gene%20of%20Erwinia%20amylovora%20in%20non%20host%20plant%20Arabidopsis%20thaliana.pdf http://irep.iium.edu.my/53950/7/53950-Expression%20of%20the%20gene%20of%20Erwinia%20amylovora%20in%20non-host%20plant%20Arabidopsis%20thaliana%20_SCOPUS.pdf

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spelling	iium-539502018-04-25T05:53:24Z http://irep.iium.edu.my/53950/ Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin QH Natural history QH301 Biology In the Erwinia amylovora genome, the hrp gene cluster containing the dspA/E/EB/F operon plays a crucial role in mediating the pathogenicity and the hypersensitive response (HR) in the host plant. The role of the dspA/E gene derived from E. amylovora was investigated by monitoring the expression of the b-glucuronidase (GUS) reporter system in transgenic Arabidopsis thaliana cv. Pri-Gus seedlings. A mutant DdspA/E strain of E. amylovora was generated to contain a deletion of the dspA/E gene for the purpose of this study. Two-week-old seedlings of GUS transgenic Arabidopsis were vacuum-infiltrated with the wild-type and the mutant (DdspA/E) E. amylovora strains. The Arabidopsis seedlings were fixed and stained for GUS activity after 3–5 days following infiltration. The appearance of dense spots with blue staining on the Arabidopsis leaves indicated the typical characteristic of GUS activity. This observation indicated that the wild-type E. amylovora strain had induced a successful and efficient infection on the A. thaliana Pri-Gus leaves. In contrast, there was no visible GUS expression on leaf tissues which were inoculated with the DdspA/E mutant E. amylovora strain. These results indicate that the dspA/E gene is required by the bacterial cells to induce HR in non-host plants. Taylor and Francis Ltd. 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53950/1/Expression%20of%20the%20dspA%20E%20gene%20of%20Erwinia%20amylovora%20in%20non%20host%20plant%20Arabidopsis%20thaliana.pdf application/pdf en http://irep.iium.edu.my/53950/7/53950-Expression%20of%20the%20gene%20of%20Erwinia%20amylovora%20in%20non-host%20plant%20Arabidopsis%20thaliana%20_SCOPUS.pdf Aksoy, Hasan Murad and Kaya, Yilmaz and Tengku Abdul Hamid, Tengku Haziyamin (2017) Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana. Biotechnology and Biotechnological Equipment, 31 (1). pp. 85-90. ISSN 1310-2818 E-ISSN 1314-3530 http://www.tandfonline.com/doi/full/10.1080/13102818.2016.1246202 10.1080/13102818.2016.1246202
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QH Natural history QH301 Biology
spellingShingle	QH Natural history QH301 Biology Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
description	In the Erwinia amylovora genome, the hrp gene cluster containing the dspA/E/EB/F operon plays a crucial role in mediating the pathogenicity and the hypersensitive response (HR) in the host plant. The role of the dspA/E gene derived from E. amylovora was investigated by monitoring the expression of the b-glucuronidase (GUS) reporter system in transgenic Arabidopsis thaliana cv. Pri-Gus seedlings. A mutant DdspA/E strain of E. amylovora was generated to contain a deletion of the dspA/E gene for the purpose of this study. Two-week-old seedlings of GUS transgenic Arabidopsis were vacuum-infiltrated with the wild-type and the mutant (DdspA/E) E. amylovora strains. The Arabidopsis seedlings were fixed and stained for GUS activity after 3–5 days following infiltration. The appearance of dense spots with blue staining on the Arabidopsis leaves indicated the typical characteristic of GUS activity. This observation indicated that the wild-type E. amylovora strain had induced a successful and efficient infection on the A. thaliana Pri-Gus leaves. In contrast, there was no visible GUS expression on leaf tissues which were inoculated with the DdspA/E mutant E. amylovora strain. These results indicate that the dspA/E gene is required by the bacterial cells to induce HR in non-host plants.
format	Article
author	Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin
author_facet	Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin
author_sort	Aksoy, Hasan Murad
title	Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
title_short	Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
title_full	Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
title_fullStr	Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
title_full_unstemmed	Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana
title_sort	expression of the dspa/e gene of erwinia amylovora in non-host plant arabidopsis thaliana
publisher	Taylor and Francis Ltd.
publishDate	2017
url	http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/ http://irep.iium.edu.my/53950/1/Expression%20of%20the%20dspA%20E%20gene%20of%20Erwinia%20amylovora%20in%20non%20host%20plant%20Arabidopsis%20thaliana.pdf http://irep.iium.edu.my/53950/7/53950-Expression%20of%20the%20gene%20of%20Erwinia%20amylovora%20in%20non-host%20plant%20Arabidopsis%20thaliana%20_SCOPUS.pdf
first_indexed	2023-09-18T21:16:20Z
last_indexed	2023-09-18T21:16:20Z
_version_	1777411586831417344

Expression of the dspA/E gene of Erwinia amylovora in non-host plant Arabidopsis thaliana

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