Molecular studies of cold active lipase and protease

Psychrophilic organisms produce enzymes adapted to function at low temperature. These enzymes are characterized by a catalytic efficiency at low and moderate temperatures but are rather thermolabile. Thermophilic enzymes usually feature in discussion of industrial uses because their heat-stability m...

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Main Authors:	Mohamad Ali, Mohd. Shukuri, Raja Abd. Rahman, Raja Noor Zaliha, Alias, Norsyuhada, Salleh, Abu Bakar, Basri, Mahiran
Format:	Book Chapter
Language:	English
Published:	Nova Science Publishers, Inc. 2013
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/54011/ http://irep.iium.edu.my/54011/ http://irep.iium.edu.my/54011/1/Book%20Chapter-Molecular%20and%20structural%20biology%20of%20new%20lipases%20an.pdf

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recordtype	eprints
spelling	iium-540112017-02-01T09:53:14Z http://irep.iium.edu.my/54011/ Molecular studies of cold active lipase and protease Mohamad Ali, Mohd. Shukuri Raja Abd. Rahman, Raja Noor Zaliha Alias, Norsyuhada Salleh, Abu Bakar Basri, Mahiran QR Microbiology Psychrophilic organisms produce enzymes adapted to function at low temperature. These enzymes are characterized by a catalytic efficiency at low and moderate temperatures but are rather thermolabile. Thermophilic enzymes usually feature in discussion of industrial uses because their heat-stability makes them ideal biocatalyst for many reactions. However, cold-active/heat-labile enzymes also have great potential in industry. Cold-active enzymes might offer novel opportunities for biotechnological exploitation based on their high catalytic activity at low temperatures, low thermostability and unusual specificities. These properties are of interest in diverse fields such as detergents, fabric and food industry, bioremediation and biocatalysts under low water conditions. Furthermore, fundamental issues concerning the molecular basis of cold activity and the interplay between flexibility and catalytic efficiency are of important in the study of structure-function relationships in protein. By means of X-ray crystallography, these properties are beginning to be understood, and the rules governing their adaptation to cold appear to be relatively diverse. To date, extensive studies on psychrophilic enzymes were conducted ranging from purification and characterization, molecular cloning and expression, homology modeling and structural studies and x-ray crystallography studies. Lipases and protease which represents the most important groups of extracellular hydrolytic enzymes were examined due to the attractive properties of these enzymes that constitute a tremendous potential for fundamental research and biotechnological applications. Nova Science Publishers, Inc. Raja Abd. Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran 2013-09-30 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/54011/1/Book%20Chapter-Molecular%20and%20structural%20biology%20of%20new%20lipases%20an.pdf Mohamad Ali, Mohd. Shukuri and Raja Abd. Rahman, Raja Noor Zaliha and Alias, Norsyuhada and Salleh, Abu Bakar and Basri, Mahiran (2013) Molecular studies of cold active lipase and protease. In: Molecular and Structural Biology of New Lipases and Proteases (Microbiology Research Advances: Biochemistry Research Trends). Nova Science Publishers, Inc., New York, pp. 89-106. ISBN 978-1626186453 https://www.novapublishers.com/catalog/product_info.php?products_id=41848
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohamad Ali, Mohd. Shukuri Raja Abd. Rahman, Raja Noor Zaliha Alias, Norsyuhada Salleh, Abu Bakar Basri, Mahiran Molecular studies of cold active lipase and protease
description	Psychrophilic organisms produce enzymes adapted to function at low temperature. These enzymes are characterized by a catalytic efficiency at low and moderate temperatures but are rather thermolabile. Thermophilic enzymes usually feature in discussion of industrial uses because their heat-stability makes them ideal biocatalyst for many reactions. However, cold-active/heat-labile enzymes also have great potential in industry. Cold-active enzymes might offer novel opportunities for biotechnological exploitation based on their high catalytic activity at low temperatures, low thermostability and unusual specificities. These properties are of interest in diverse fields such as detergents, fabric and food industry, bioremediation and biocatalysts under low water conditions. Furthermore, fundamental issues concerning the molecular basis of cold activity and the interplay between flexibility and catalytic efficiency are of important in the study of structure-function relationships in protein. By means of X-ray crystallography, these properties are beginning to be understood, and the rules governing their adaptation to cold appear to be relatively diverse. To date, extensive studies on psychrophilic enzymes were conducted ranging from purification and characterization, molecular cloning and expression, homology modeling and structural studies and x-ray crystallography studies. Lipases and protease which represents the most important groups of extracellular hydrolytic enzymes were examined due to the attractive properties of these enzymes that constitute a tremendous potential for fundamental research and biotechnological applications.
author2	Raja Abd. Rahman, Raja Noor Zaliha
author_facet	Raja Abd. Rahman, Raja Noor Zaliha Mohamad Ali, Mohd. Shukuri Raja Abd. Rahman, Raja Noor Zaliha Alias, Norsyuhada Salleh, Abu Bakar Basri, Mahiran
format	Book Chapter
author	Mohamad Ali, Mohd. Shukuri Raja Abd. Rahman, Raja Noor Zaliha Alias, Norsyuhada Salleh, Abu Bakar Basri, Mahiran
author_sort	Mohamad Ali, Mohd. Shukuri
title	Molecular studies of cold active lipase and protease
title_short	Molecular studies of cold active lipase and protease
title_full	Molecular studies of cold active lipase and protease
title_fullStr	Molecular studies of cold active lipase and protease
title_full_unstemmed	Molecular studies of cold active lipase and protease
title_sort	molecular studies of cold active lipase and protease
publisher	Nova Science Publishers, Inc.
publishDate	2013
url	http://irep.iium.edu.my/54011/ http://irep.iium.edu.my/54011/ http://irep.iium.edu.my/54011/1/Book%20Chapter-Molecular%20and%20structural%20biology%20of%20new%20lipases%20an.pdf
first_indexed	2023-09-18T21:16:25Z
last_indexed	2023-09-18T21:16:25Z
_version_	1777411592364752896

Molecular studies of cold active lipase and protease

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