Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia

Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging f...

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Main Authors:	Mohd. Ridah, Lailatul Jalilah, Ismail, Aziah, A. Talib, Norlelawati, Muhammad, Siti Aesah @ Naznin, Hussain, Faezahtul Arbaiyah, Zainuddin, Norafiza
Format:	Article
Language:	English
Published:	Universiti Sains Malaysia 2016
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf

id	iium-54196
recordtype	eprints
spelling	iium-541962017-01-20T01:13:46Z http://irep.iium.edu.my/54196/ Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza RC0254 Neoplasms. Tumors. Oncology (including Cancer) Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging from 16 - 54%. However, quantitative analysis of p16 promoter methylation in DLBCL has not been extensively studied in Malaysia. Objective: This study aims to quantitatively analyse p16 methylation in DLBCL samples using pyrosequencing technique. Methods: Genomic DNA was extracted from 16 formalin-fixed paraffin-embedded lymphoma tissue blocks from patients diagnosed with DLBCL. Samples were retrieved from Hospital Tengku Ampuan Afzan, Pahang and Hospital Universiti Sains Malaysia, Kelantan. Primers were designed to amplify bisulfite-treated DNA targeting p16 promoter region. Methylation status of 7 CpG sites was determined by pyrosequencing. Results: All the 16 samples studied showed promoter methylation of p16. The range of mean methylation percentage was between 18 to 81%. Conclusion: The present study has successfully measured the level of methylation of p16 in all 7 CpG sites despite the limitation in sample size. Since p16 methylation is a common event in our series of DLBCL cases, it is worth including a larger sample size in future studies to increase the chance of finding a significant correlation with clinical parameters. Universiti Sains Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf Mohd. Ridah, Lailatul Jalilah and Ismail, Aziah and A. Talib, Norlelawati and Muhammad, Siti Aesah @ Naznin and Hussain, Faezahtul Arbaiyah and Zainuddin, Norafiza (2016) Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia. Journal of Biomedical and Clinical Sciences, 1 (1). pp. 12-17. E-ISSN 2550-147X http://apps.amdi.usm.my/journal/index.php/jbcs/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
description	Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging from 16 - 54%. However, quantitative analysis of p16 promoter methylation in DLBCL has not been extensively studied in Malaysia. Objective: This study aims to quantitatively analyse p16 methylation in DLBCL samples using pyrosequencing technique. Methods: Genomic DNA was extracted from 16 formalin-fixed paraffin-embedded lymphoma tissue blocks from patients diagnosed with DLBCL. Samples were retrieved from Hospital Tengku Ampuan Afzan, Pahang and Hospital Universiti Sains Malaysia, Kelantan. Primers were designed to amplify bisulfite-treated DNA targeting p16 promoter region. Methylation status of 7 CpG sites was determined by pyrosequencing. Results: All the 16 samples studied showed promoter methylation of p16. The range of mean methylation percentage was between 18 to 81%. Conclusion: The present study has successfully measured the level of methylation of p16 in all 7 CpG sites despite the limitation in sample size. Since p16 methylation is a common event in our series of DLBCL cases, it is worth including a larger sample size in future studies to increase the chance of finding a significant correlation with clinical parameters.
format	Article
author	Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza
author_facet	Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aesah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza
author_sort	Mohd. Ridah, Lailatul Jalilah
title	Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
title_short	Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
title_full	Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
title_fullStr	Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
title_full_unstemmed	Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
title_sort	pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia
publisher	Universiti Sains Malaysia
publishDate	2016
url	http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf
first_indexed	2023-09-18T21:16:40Z
last_indexed	2023-09-18T21:16:40Z
_version_	1777411608805376000

Pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia

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