Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
Asiaticoside, is one of the bioactive constituents in Centella asiatica, has been reported to have wound healing properties. Biotransformation was extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmac...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/54232/ http://irep.iium.edu.my/54232/1/Prof%20NO%20presentation%20ICNP2016%20update.pdf http://irep.iium.edu.my/54232/31/54232%20tentative.pdf |
| Summary: | Asiaticoside, is one of the bioactive constituents in Centella asiatica, has been reported to have
wound healing properties. Biotransformation was extensively used to create useful metabolites
from various natural products and as an alternative to chemical synthesis for the preparation of
pharmacologically active compounds. The purpose of this study is to investigate the
effects of asiaticoside and its transformed product on wound healing activities.
Microbial transformation of asiaticoside using Aspergillus niger was carried out to
produce an asiaticoside transformed product which has pharmaceutical properties.
The wound healing activities of asiaticoside and its transformed product were
investigated and their effects on TGFβ1 and TIMP1 gene expression were examined
in order to understand their mode of action on healing. Asiaticoside and its
transformed product were also used in the cell proliferation and wound healing
experiments using human keratinocytes. Results of in vitro study showed that
asiaticoside concentrations between 7.5 and 120 μg/ml gave higher value of cell
proliferation than the negative control, prompting cell proliferation improvement. On
the other hand, it was found that the transformed product concentrations between
0.058 and 3.75 μg/ml exhibited the higher cell viability values. In wound healing
assays analysis, it was shown that there were significant differences on wound closure
in comparison with the negative control (P<0.05). Analysis showed that both
asiaticoside and the transformed product increased the expression of TGFβ1 and
TIMP1 respectively. However, the transformed product was more effective than
asiaticoside on the expression of TGFβ1 and TIMP1 respectively. It was observed
that the asiaticoside-transformed product showed a faster migration and healing
speed under macroscopic observation. |
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