Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product

Asiaticoside, is one of the bioactive constituents in Centella asiatica, has been reported to have wound healing properties. Biotransformation was extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmac...

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Main Authors:	Omar, Muhammad Nor, Alfarra, Helmi Yousif, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/54232/ http://irep.iium.edu.my/54232/1/Prof%20NO%20presentation%20ICNP2016%20update.pdf http://irep.iium.edu.my/54232/31/54232%20tentative.pdf

id	iium-54232
recordtype	eprints
spelling	iium-542322017-02-02T07:28:33Z http://irep.iium.edu.my/54232/ Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product Omar, Muhammad Nor Alfarra, Helmi Yousif Arief Ichwan, Solachuddin Jauhari QD Chemistry Asiaticoside, is one of the bioactive constituents in Centella asiatica, has been reported to have wound healing properties. Biotransformation was extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmacologically active compounds. The purpose of this study is to investigate the effects of asiaticoside and its transformed product on wound healing activities. Microbial transformation of asiaticoside using Aspergillus niger was carried out to produce an asiaticoside transformed product which has pharmaceutical properties. The wound healing activities of asiaticoside and its transformed product were investigated and their effects on TGFβ1 and TIMP1 gene expression were examined in order to understand their mode of action on healing. Asiaticoside and its transformed product were also used in the cell proliferation and wound healing experiments using human keratinocytes. Results of in vitro study showed that asiaticoside concentrations between 7.5 and 120 μg/ml gave higher value of cell proliferation than the negative control, prompting cell proliferation improvement. On the other hand, it was found that the transformed product concentrations between 0.058 and 3.75 μg/ml exhibited the higher cell viability values. In wound healing assays analysis, it was shown that there were significant differences on wound closure in comparison with the negative control (P<0.05). Analysis showed that both asiaticoside and the transformed product increased the expression of TGFβ1 and TIMP1 respectively. However, the transformed product was more effective than asiaticoside on the expression of TGFβ1 and TIMP1 respectively. It was observed that the asiaticoside-transformed product showed a faster migration and healing speed under macroscopic observation. 2016-03 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/54232/1/Prof%20NO%20presentation%20ICNP2016%20update.pdf application/pdf en http://irep.iium.edu.my/54232/31/54232%20tentative.pdf Omar, Muhammad Nor and Alfarra, Helmi Yousif and Arief Ichwan, Solachuddin Jauhari (2016) Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product. In: International Conference on Natural Product (ICNP 2016), 15th-17th March 2016, Kuala Trengganu, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Omar, Muhammad Nor Alfarra, Helmi Yousif Arief Ichwan, Solachuddin Jauhari Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
description	Asiaticoside, is one of the bioactive constituents in Centella asiatica, has been reported to have wound healing properties. Biotransformation was extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmacologically active compounds. The purpose of this study is to investigate the effects of asiaticoside and its transformed product on wound healing activities. Microbial transformation of asiaticoside using Aspergillus niger was carried out to produce an asiaticoside transformed product which has pharmaceutical properties. The wound healing activities of asiaticoside and its transformed product were investigated and their effects on TGFβ1 and TIMP1 gene expression were examined in order to understand their mode of action on healing. Asiaticoside and its transformed product were also used in the cell proliferation and wound healing experiments using human keratinocytes. Results of in vitro study showed that asiaticoside concentrations between 7.5 and 120 μg/ml gave higher value of cell proliferation than the negative control, prompting cell proliferation improvement. On the other hand, it was found that the transformed product concentrations between 0.058 and 3.75 μg/ml exhibited the higher cell viability values. In wound healing assays analysis, it was shown that there were significant differences on wound closure in comparison with the negative control (P<0.05). Analysis showed that both asiaticoside and the transformed product increased the expression of TGFβ1 and TIMP1 respectively. However, the transformed product was more effective than asiaticoside on the expression of TGFβ1 and TIMP1 respectively. It was observed that the asiaticoside-transformed product showed a faster migration and healing speed under macroscopic observation.
format	Conference or Workshop Item
author	Omar, Muhammad Nor Alfarra, Helmi Yousif Arief Ichwan, Solachuddin Jauhari
author_facet	Omar, Muhammad Nor Alfarra, Helmi Yousif Arief Ichwan, Solachuddin Jauhari
author_sort	Omar, Muhammad Nor
title	Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
title_short	Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
title_full	Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
title_fullStr	Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
title_full_unstemmed	Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product
title_sort	biotransformation of asiaticoside using aspergillus niger and pharmaceutical properties of its transformed product
publishDate	2016
url	http://irep.iium.edu.my/54232/ http://irep.iium.edu.my/54232/1/Prof%20NO%20presentation%20ICNP2016%20update.pdf http://irep.iium.edu.my/54232/31/54232%20tentative.pdf
first_indexed	2023-09-18T21:16:44Z
last_indexed	2023-09-18T21:16:44Z
_version_	1777411612285599744

Biotransformation of asiaticoside using Aspergillus niger and pharmaceutical properties of its transformed product

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