In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud

In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud

Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration...

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Main Authors: Zainuddin, Zarina, Mohd Kamil, Asmaa'
Format: Article
Language:English
Published: Zibeline International Publishing 2019
Subjects:
QK Botany
S Agriculture (General)
SB Plant culture
Online Access:http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/1/54586_In-vitro%20regeneration%20of%20orthosiphon.pdf
id iium-54586
recordtype eprints
spelling iium-545862019-07-12T08:03:38Z http://irep.iium.edu.my/54586/ In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud Zainuddin, Zarina Mohd Kamil, Asmaa' QK Botany S Agriculture (General) SB Plant culture Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique. Zibeline International Publishing 2019-02-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54586/1/54586_In-vitro%20regeneration%20of%20orthosiphon.pdf Zainuddin, Zarina and Mohd Kamil, Asmaa' (2019) In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud. Science Heritage Journal (GWS), 3 (1). pp. 8-10. ISSN 2521-0858 E-ISSN 2521-0866 https://zibelinepub.com/archives/1gws2019/1gws2019-08-10.pdf 10.26480/gws.01.2019.08.10
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QK Botany
S Agriculture (General)
SB Plant culture
spellingShingle QK Botany
S Agriculture (General)
SB Plant culture
Zainuddin, Zarina
Mohd Kamil, Asmaa'
In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
description Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique.
format Article
author Zainuddin, Zarina
Mohd Kamil, Asmaa'
author_facet Zainuddin, Zarina
Mohd Kamil, Asmaa'
author_sort Zainuddin, Zarina
title In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
title_short In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
title_full In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
title_fullStr In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
title_full_unstemmed In-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
title_sort in-vitro regeneration of orthosiphon stamineus (misai kucing) using axillary bud
publisher Zibeline International Publishing
publishDate 2019
url http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/
http://irep.iium.edu.my/54586/1/54586_In-vitro%20regeneration%20of%20orthosiphon.pdf
first_indexed 2023-09-18T21:17:13Z
last_indexed 2023-09-18T21:17:13Z
_version_ 1777411642978467840

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