Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides

Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQ...

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Main Authors:	Abdul Wahab, Widya, Šuligoj, Tanja, Ellis, Julia H., Côrtez-Real, Beatriz, Ciclitira, Paul Jonathan
Format:	Article
Language:	English English
Published:	Wiley-Blackwell 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf

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spelling	iium-547472018-05-02T05:55:45Z http://irep.iium.edu.my/54747/ Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides Abdul Wahab, Widya Šuligoj, Tanja Ellis, Julia H. Côrtez-Real, Beatriz Ciclitira, Paul Jonathan Q Science (General) Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic–tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic–tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared. © 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology Wiley-Blackwell 2016-08-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf application/pdf en http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf Abdul Wahab, Widya and Šuligoj, Tanja and Ellis, Julia H. and Côrtez-Real, Beatriz and Ciclitira, Paul Jonathan (2016) Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides. International Journal of Experimental Pathology, 97 (4). pp. 303-309. ISSN 0959-9673 E-ISSN 1365-2613 http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2613 10.1111/iep.12199
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdul Wahab, Widya Šuligoj, Tanja Ellis, Julia H. Côrtez-Real, Beatriz Ciclitira, Paul Jonathan Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
description	Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin peptide (QPFPQPQQPIPQ) were proposed to be toxic in CD, as they yielded positive responses when assessed with peripheral blood T-cell clones derived from individuals with CD. We sought to assess the immunogenicity of the candidate peptides using gluten-sensitive T-cell lines obtained from CD small intestinal biopsies. We also sought to investigate the potential cross-reactivity of wheat gluten-sensitive T-cell lines with peptic–tryptic digested barley hordein (PTH) and rye secalin (PTS). Synthesised candidate peptides were deamidated with tissue transglutaminase (tTG). Gluten-sensitive T-cell lines were generated by culturing small intestinal biopsies from CD patients with peptic–tryptic gluten (PTG), PTH or PTS, along with autologous PBMCs for antigen presentation. The stimulation indices were determined by measuring the relative cellular proliferation via incorporation of 3H-thymidine. The majority of T-cell lines reacted to the peptides studied. There was also cross-reactivity between wheat gluten-sensitive T-cell lines and the hordein, gliadin and secalin peptides. PTH, PTS, barley hordein and rye secalin-derived CD antigen-sensitive T-cell lines showed positive stimulation with PTG. ω-gliadin/C-hordein peptide and rye-derived peptide are immunogenic to gluten-sensitive T-cell lines and potentially present in wheat, rye and barley. Additional CD toxic peptides may be shared. © 2016 The Authors. International Journal of Experimental Pathology © 2016 International Journal of Experimental Pathology
format	Article
author	Abdul Wahab, Widya Šuligoj, Tanja Ellis, Julia H. Côrtez-Real, Beatriz Ciclitira, Paul Jonathan
author_facet	Abdul Wahab, Widya Šuligoj, Tanja Ellis, Julia H. Côrtez-Real, Beatriz Ciclitira, Paul Jonathan
author_sort	Abdul Wahab, Widya
title	Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_short	Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_full	Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_fullStr	Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_full_unstemmed	Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
title_sort	coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides
publisher	Wiley-Blackwell
publishDate	2016
url	http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/ http://irep.iium.edu.my/54747/9/54747_Coeliac%20disease_article.pdf http://irep.iium.edu.my/54747/2/54747_Coeliac%20disease_SCOPUS.pdf
first_indexed	2023-09-18T21:17:25Z
last_indexed	2023-09-18T21:17:25Z
_version_	1777411655953547264

Coeliac disease: immunogenicity studies of barley hordein and rye secalin-derived peptides

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