Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell

Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has show...

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Main Authors:	Mel, Maizirwan, Mat Saad, Maizura, Hashim, Yumi Zuhanis Has-Yun, Mohamed Salleh, Moharnad Ramlan
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2008
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5476/ http://irep.iium.edu.my/5476/ http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf

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spelling	iium-54762017-08-02T08:59:49Z http://irep.iium.edu.my/5476/ Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan TP248.13 Biotechnology Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaC03 concentration, respectively. Among the 16 nillS tested, Rllll 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaC03, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO, were 0.000069, 0.003968 and 0.342151 (R' ~ 0.95476 and R Adj. ~ 0.88691), respectively. Asian Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf Mel, Maizirwan and Mat Saad, Maizura and Hashim, Yumi Zuhanis Has-Yun and Mohamed Salleh, Moharnad Ramlan (2008) Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell. Asian Journal of Scientific Research, 1 (5). pp. 525-531. ISSN 1992-1454 http://docsdrive.com/pdfs/ansinet/ajsr/2008/525-531.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
description	Media optimization of RCI hybridoma cell culture for monoclonal antibody production was carried out in T -Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology(STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaC03 concentration, respectively. Among the 16 nillS tested, Rllll 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaC03, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO, were 0.000069, 0.003968 and 0.342151 (R' ~ 0.95476 and R Adj. ~ 0.88691), respectively.
format	Article
author	Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan
author_facet	Mel, Maizirwan Mat Saad, Maizura Hashim, Yumi Zuhanis Has-Yun Mohamed Salleh, Moharnad Ramlan
author_sort	Mel, Maizirwan
title	Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
title_short	Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
title_full	Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
title_fullStr	Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
title_full_unstemmed	Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
title_sort	monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
publisher	Asian Network for Scientific Information
publishDate	2008
url	http://irep.iium.edu.my/5476/ http://irep.iium.edu.my/5476/ http://irep.iium.edu.my/5476/1/1957-AJSR_%28Published_On-Line%29.pdf
first_indexed	2023-09-18T20:14:05Z
last_indexed	2023-09-18T20:14:05Z
_version_	1777407671356358656

Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell

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