Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms

Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of ch...

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Main Authors:	Shahdan, Intan Azura, Mohd Sobri, Fatimah Zahra, Hanapi, Mohammad Faiz Hizzuan, Ahmad Yusof @ Hanafi, Hanani, How, Fiona Ni Foong
Format:	Conference or Workshop Item
Language:	English
Published:	International Islamic University Malaysia 2016
Subjects:	QD Chemistry QR Microbiology
Online Access:	http://irep.iium.edu.my/54958/ http://irep.iium.edu.my/54958/ http://irep.iium.edu.my/54958/1/54958_International%20medical_complete.pdf

id	iium-54958
recordtype	eprints
spelling	iium-549582017-04-14T06:45:47Z http://irep.iium.edu.my/54958/ Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms Shahdan, Intan Azura Mohd Sobri, Fatimah Zahra Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong QD Chemistry QR Microbiology Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed. International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54958/1/54958_International%20medical_complete.pdf Shahdan, Intan Azura and Mohd Sobri, Fatimah Zahra and Hanapi, Mohammad Faiz Hizzuan and Ahmad Yusof @ Hanafi, Hanani and How, Fiona Ni Foong (2016) Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/vol-15-supplement-issue/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry QR Microbiology
spellingShingle	QD Chemistry QR Microbiology Shahdan, Intan Azura Mohd Sobri, Fatimah Zahra Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
description	Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed.
format	Conference or Workshop Item
author	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahra Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_facet	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahra Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_sort	Shahdan, Intan Azura
title	Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
title_short	Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
title_full	Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
title_fullStr	Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
title_full_unstemmed	Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
title_sort	antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms
publisher	International Islamic University Malaysia
publishDate	2016
url	http://irep.iium.edu.my/54958/ http://irep.iium.edu.my/54958/ http://irep.iium.edu.my/54958/1/54958_International%20medical_complete.pdf
first_indexed	2023-09-18T21:17:42Z
last_indexed	2023-09-18T21:17:42Z
_version_	1777411673741590528

Antibiotic susceptibility study of metal-gentamycin complexes against pseudomonas aeruginosa biofilms

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