In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress

Objectives/Research Problem: Degenerative joint disease normally affects older population which eventually disturb patient’s mobility. Current available prescriptions and surgical procedures are incapable to cure the disease completely due to the nature of avascular cartilage. Gene transfer and ti...

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Main Authors:	Md Nazir, Noorhidayah, Zulkifly, Ahmad Hafiz, Khalid, Kamarul Ariffin, Zainol, Ismail, Zamli, Zaitunnatakhin, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Allied Health Sciences, IIUM 2016
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/55024/ http://irep.iium.edu.my/55024/1/Noorhidayah%20KRW2016%20Abstract.pdf

id	iium-55024
recordtype	eprints
spelling	iium-550242018-05-18T07:29:30Z http://irep.iium.edu.my/55024/ In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Zainol, Ismail Zamli, Zaitunnatakhin Sha'ban, Munirah TA164 Bioengineering Objectives/Research Problem: Degenerative joint disease normally affects older population which eventually disturb patient’s mobility. Current available prescriptions and surgical procedures are incapable to cure the disease completely due to the nature of avascular cartilage. Gene transfer and tissue engineering have emerged as promising approaches in treating the disease. The idea behind the incorporation of the two approaches is to regenerate a stable tissue engineered cartilage constructs by enhancing the cells source. Hence, the aim of this study is to assess the effect of non-viral SOX9 and TERT genes transfected chondrocytes in vitro and in vivo. Materials and Method: With the approval of Institutional Animal Care and Use Committee (IACUCIIUM) (IIUM/IACUC/Approval 2015/(5)/(24)), SOX9 and TERT genes are transferred in monolayer cultured chondrocytes via lipofection technique. The post-transfected chondrocytes are then seeded in a three-dimensional (3D) poly(lactic-co-glycolic) (PLGA) with and without fibrin scaffolds for tissue engineered cartilage constructs formation in vitro. The resulting constructs are implanted subcutaneously in the athymic nude mice for constructs’ maturation purpose. The incorporation of cells in the 3D scaffold in vitro and the in vivo implantation are important to mimic the internal microenvironment of the human body. The monolayer post-transfected chondrocytes, in vitro and in vivo 3D constructs are evaluated with several different assessments namely glycosaminoglycan (GAG) assay, cell proliferation assay, histological and immunohistochemistry, gene expression assessment. This is done to confirm the chondrogenic properties of the constructs based on selected time points. Results and Discussion: Several preliminary findings are obtained. The results show certain trend towards synergistic effect of both SOX9 and TERT genes on chondrocytes. However, no conclusion can be made at this juncture. The experiment is still ongoing. Conclusion: It is hoped that the combination of SOX9 and TERT genes can help regenerate a good quality cartilage for tissue engineering purpose. Kulliyyah of Allied Health Sciences, IIUM 2016-11-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55024/1/Noorhidayah%20KRW2016%20Abstract.pdf Md Nazir, Noorhidayah and Zulkifly, Ahmad Hafiz and Khalid, Kamarul Ariffin and Zainol, Ismail and Zamli, Zaitunnatakhin and Sha'ban, Munirah (2016) In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21 - 25 November 2016, Kuantan.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Zainol, Ismail Zamli, Zaitunnatakhin Sha'ban, Munirah In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
description	Objectives/Research Problem: Degenerative joint disease normally affects older population which eventually disturb patient’s mobility. Current available prescriptions and surgical procedures are incapable to cure the disease completely due to the nature of avascular cartilage. Gene transfer and tissue engineering have emerged as promising approaches in treating the disease. The idea behind the incorporation of the two approaches is to regenerate a stable tissue engineered cartilage constructs by enhancing the cells source. Hence, the aim of this study is to assess the effect of non-viral SOX9 and TERT genes transfected chondrocytes in vitro and in vivo. Materials and Method: With the approval of Institutional Animal Care and Use Committee (IACUCIIUM) (IIUM/IACUC/Approval 2015/(5)/(24)), SOX9 and TERT genes are transferred in monolayer cultured chondrocytes via lipofection technique. The post-transfected chondrocytes are then seeded in a three-dimensional (3D) poly(lactic-co-glycolic) (PLGA) with and without fibrin scaffolds for tissue engineered cartilage constructs formation in vitro. The resulting constructs are implanted subcutaneously in the athymic nude mice for constructs’ maturation purpose. The incorporation of cells in the 3D scaffold in vitro and the in vivo implantation are important to mimic the internal microenvironment of the human body. The monolayer post-transfected chondrocytes, in vitro and in vivo 3D constructs are evaluated with several different assessments namely glycosaminoglycan (GAG) assay, cell proliferation assay, histological and immunohistochemistry, gene expression assessment. This is done to confirm the chondrogenic properties of the constructs based on selected time points. Results and Discussion: Several preliminary findings are obtained. The results show certain trend towards synergistic effect of both SOX9 and TERT genes on chondrocytes. However, no conclusion can be made at this juncture. The experiment is still ongoing. Conclusion: It is hoped that the combination of SOX9 and TERT genes can help regenerate a good quality cartilage for tissue engineering purpose.
format	Conference or Workshop Item
author	Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Zainol, Ismail Zamli, Zaitunnatakhin Sha'ban, Munirah
author_facet	Md Nazir, Noorhidayah Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Zainol, Ismail Zamli, Zaitunnatakhin Sha'ban, Munirah
author_sort	Md Nazir, Noorhidayah
title	In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
title_short	In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
title_full	In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
title_fullStr	In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
title_full_unstemmed	In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
title_sort	in vitro and in vivo non-viral sry (sex determining region y)-box 9 (sox9) and telomerase reverse transcriptase (tert) genes transfer in chondrocytes: work in progress
publisher	Kulliyyah of Allied Health Sciences, IIUM
publishDate	2016
url	http://irep.iium.edu.my/55024/ http://irep.iium.edu.my/55024/1/Noorhidayah%20KRW2016%20Abstract.pdf
first_indexed	2023-09-18T21:17:47Z
last_indexed	2023-09-18T21:17:47Z
_version_	1777411678563991552

In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress

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