Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold

Objectives/Research Problem: Poly(lactic-co-glycolic acid) (PLGA), an FDA-approved, synthetic copolymer has been widely applied in clinical settings as one of the suturing materials. It has been used as cartilage tissue engineering scaffolds because it is bioabsorbable and safe for human use. PLGA c...

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Main Authors:	Mohd. Azharuddin, Nur Syamimi, Shuib, Noor Faizatul Husna, Md Nazir, Noorhidayah, Mohamad, Mohd Yusof, Mohamed Amin, Muhammad Azri Ifwat, Ahmad Radzi, Muhammad Aa’zamuddin, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Allied Health Sciences, IIUM 2016
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/55025/ http://irep.iium.edu.my/55025/1/Syamimi%20KRW2016%20Abstract.pdf

id	iium-55025
recordtype	eprints
spelling	iium-550252017-02-12T13:25:09Z http://irep.iium.edu.my/55025/ Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold Mohd. Azharuddin, Nur Syamimi Shuib, Noor Faizatul Husna Md Nazir, Noorhidayah Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Ahmad Radzi, Muhammad Aa’zamuddin Sha'ban, Munirah TA164 Bioengineering Objectives/Research Problem: Poly(lactic-co-glycolic acid) (PLGA), an FDA-approved, synthetic copolymer has been widely applied in clinical settings as one of the suturing materials. It has been used as cartilage tissue engineering scaffolds because it is bioabsorbable and safe for human use. PLGA can be prepared alone or in combination with other biomaterials such as fibrin to enhance the surface-adhesion properties. Fibrin helps to hold the cells inside the scaffolds and thus minimizes cells lost. This condition facilitates homogeneous cells distribution and extracellular matrix (ECM) production. This study aimed to evaluate in vitro constructs engineered from PLGA seeded with intervertebral disc (IVD) cells namely annulus fibrosus (AF) cell, nucleus pulposus (NP) cell, and a combination of AF:NP (1:1) with and without fibrin using histology staining. Materials and Method: All cell groups were cultured until passage 1 (P1) prior to seeding step onto the pre-fabricated PLGA scaffolds. Approx. 1.0x106 cells were used per scaffold. The resulted “cells-scaffolds” constructs were cultured for 3-weeks. The microscopic evaluation using H&E, Alcian Blue and Safranin O staining was performed on all constructs at weeks 1, 2 and 3. Results and Discussion: The overall results suggested minimal formation of cartilaginous tissue at week 1 until week 3 in all groups. Formation of cartilaginous tissue is indicated by the presence of cartilage-isolated cells in lacunae spaces. PLGA+Fibrin seeded with AF:NP demonstrated better cellular and ECM distribution than the other PLGA based constructs. Glycosaminoglycan (GAG) accumulation is noted in most constructs using Alcian Blue staining. However, the presence of proteoglycan-rich matrix was not detected in most constructs using Safranin O staining. This may be due to the immature nature of the in vitro tissue constructs. They have yet to produce cartilage specific proteoglycan-rich matrix. Conclusion: This preliminary study showed that PLGA+Fibrin has the potential to promote early formation of in vitro cartilaginous constructs engineered from the IVD cells. It is hoped that the findings provide a useful information for future research in IVD tissue engineering. Kulliyyah of Allied Health Sciences, IIUM 2016-11-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55025/1/Syamimi%20KRW2016%20Abstract.pdf Mohd. Azharuddin, Nur Syamimi and Shuib, Noor Faizatul Husna and Md Nazir, Noorhidayah and Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Ahmad Radzi, Muhammad Aa’zamuddin and Sha'ban, Munirah (2016) Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold. In: KAHS Research Week (KRW) 2016 in conjunction with 1st Allied Health Scientific Colloquium (AHSC) 2016, 21-25 Nov 2016, Kuantan.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Mohd. Azharuddin, Nur Syamimi Shuib, Noor Faizatul Husna Md Nazir, Noorhidayah Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Ahmad Radzi, Muhammad Aa’zamuddin Sha'ban, Munirah Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
description	Objectives/Research Problem: Poly(lactic-co-glycolic acid) (PLGA), an FDA-approved, synthetic copolymer has been widely applied in clinical settings as one of the suturing materials. It has been used as cartilage tissue engineering scaffolds because it is bioabsorbable and safe for human use. PLGA can be prepared alone or in combination with other biomaterials such as fibrin to enhance the surface-adhesion properties. Fibrin helps to hold the cells inside the scaffolds and thus minimizes cells lost. This condition facilitates homogeneous cells distribution and extracellular matrix (ECM) production. This study aimed to evaluate in vitro constructs engineered from PLGA seeded with intervertebral disc (IVD) cells namely annulus fibrosus (AF) cell, nucleus pulposus (NP) cell, and a combination of AF:NP (1:1) with and without fibrin using histology staining. Materials and Method: All cell groups were cultured until passage 1 (P1) prior to seeding step onto the pre-fabricated PLGA scaffolds. Approx. 1.0x106 cells were used per scaffold. The resulted “cells-scaffolds” constructs were cultured for 3-weeks. The microscopic evaluation using H&E, Alcian Blue and Safranin O staining was performed on all constructs at weeks 1, 2 and 3. Results and Discussion: The overall results suggested minimal formation of cartilaginous tissue at week 1 until week 3 in all groups. Formation of cartilaginous tissue is indicated by the presence of cartilage-isolated cells in lacunae spaces. PLGA+Fibrin seeded with AF:NP demonstrated better cellular and ECM distribution than the other PLGA based constructs. Glycosaminoglycan (GAG) accumulation is noted in most constructs using Alcian Blue staining. However, the presence of proteoglycan-rich matrix was not detected in most constructs using Safranin O staining. This may be due to the immature nature of the in vitro tissue constructs. They have yet to produce cartilage specific proteoglycan-rich matrix. Conclusion: This preliminary study showed that PLGA+Fibrin has the potential to promote early formation of in vitro cartilaginous constructs engineered from the IVD cells. It is hoped that the findings provide a useful information for future research in IVD tissue engineering.
format	Conference or Workshop Item
author	Mohd. Azharuddin, Nur Syamimi Shuib, Noor Faizatul Husna Md Nazir, Noorhidayah Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Ahmad Radzi, Muhammad Aa’zamuddin Sha'ban, Munirah
author_facet	Mohd. Azharuddin, Nur Syamimi Shuib, Noor Faizatul Husna Md Nazir, Noorhidayah Mohamad, Mohd Yusof Mohamed Amin, Muhammad Azri Ifwat Ahmad Radzi, Muhammad Aa’zamuddin Sha'ban, Munirah
author_sort	Mohd. Azharuddin, Nur Syamimi
title	Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
title_short	Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
title_full	Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
title_fullStr	Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
title_full_unstemmed	Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
title_sort	histology staining on in vitro 3d poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
publisher	Kulliyyah of Allied Health Sciences, IIUM
publishDate	2016
url	http://irep.iium.edu.my/55025/ http://irep.iium.edu.my/55025/1/Syamimi%20KRW2016%20Abstract.pdf
first_indexed	2023-09-18T21:17:47Z
last_indexed	2023-09-18T21:17:47Z
_version_	1777411678708695040

Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold

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