Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients

Background and Purpose: Klebsiella pneumoniae and Klebsiella oxytoca are the two most common pathogens causing nosocomial infections in humans and are of great concern for developing multidrug resistance. In the present study, K. pneumoniae and K. oxytoca from clinical samples were evaluated for the...

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Main Authors:	Sourav, Chakrabarty, Mohsina, Kaniz, Kumar Sarker, Palash, Alam, Md Zahangir, Abdul Karim, Mohamed Ismail, Abu Sayem, S.M
Format:	Article
Language:	English English English
Published:	Croatian Society of Natural Sciences 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/1/Paper%20Antiboitic%20Sayem.pdf http://irep.iium.edu.my/55043/7/55043_Prevalence%2Cantibiotic_SCOPUS.pdf http://irep.iium.edu.my/55043/8/55043_Prevalence%2Cantibiotic_SCOPUS_WOS.pdf

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spelling	iium-550432017-08-10T01:15:19Z http://irep.iium.edu.my/55043/ Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients Sourav, Chakrabarty Mohsina, Kaniz Kumar Sarker, Palash Alam, Md Zahangir Abdul Karim, Mohamed Ismail Abu Sayem, S.M TP248.13 Biotechnology Background and Purpose: Klebsiella pneumoniae and Klebsiella oxytoca are the two most common pathogens causing nosocomial infections in humans and are of great concern for developing multidrug resistance. In the present study, K. pneumoniae and K. oxytoca from clinical samples were evaluated for their antibiotic sensitivity patterns against commonly used antibiotics and production of extended-spectrum beta-lactamase (ESBL). Materials and Methods: The isolates were obtained from tracheal swabs, sputum, wound swabs, pus, blood and urine samples of hospitalized patients. Klebsiella pneumoniae and Klebsiella oxytoca were identified by cultural and biochemical methods. Antibiotic sensitivity test was performed by modified Kirby-Bauer disc diffusion technique. ESBL production in Klebsiella spp. was confirmed by double disc synergy test. Results and Conclusion: Out of 500 clinical isolates, 120 were found positive for Klebsiella among which 108 were K. pneumoniae and 12 were K. oxytoca based on indole test. Prevalence rate of Klebsiella was found more prominent in males aged over 50 years, mostly in urine samples. Overall resistance pattern of Klebsiella isolates to ampicillin,amoxicillin, ceftriaxone, ciprofloxacin, co-trimoxazole, gentamicin, nalidixic acid,ttracycline was 100%, 90%, 45%, 40%, 45%, 25%, 50%, 35% respectively. Multidrug resistance was found more common in K. pneumoniae (56%) than in K. oxytoca (50%). Prevalence rate of ESBL producing Klebsiella was found 45% among which K. pneumoniae (50%) were found more prominent than K. oxytoca (25%). All the ESBL producing Klebsiella isolates were found to be multidrug resistant, showing 100% resistance to Ampicillin,Amoxicillin,Ceftriaxone and Ciprofloxacin. Croatian Society of Natural Sciences 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55043/1/Paper%20Antiboitic%20Sayem.pdf application/pdf en http://irep.iium.edu.my/55043/7/55043_Prevalence%2Cantibiotic_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/55043/8/55043_Prevalence%2Cantibiotic_SCOPUS_WOS.pdf Sourav, Chakrabarty and Mohsina, Kaniz and Kumar Sarker, Palash and Alam, Md Zahangir and Abdul Karim, Mohamed Ismail and Abu Sayem, S.M (2016) Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients. Periodicum Biologorum, 118 (1). pp. 53-58. ISSN 00315362 http://doi.org/10.18054/pb.2016.118.1.3160 doi:10.18054/pb.2016.118.1.3160
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Sourav, Chakrabarty Mohsina, Kaniz Kumar Sarker, Palash Alam, Md Zahangir Abdul Karim, Mohamed Ismail Abu Sayem, S.M Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
description	Background and Purpose: Klebsiella pneumoniae and Klebsiella oxytoca are the two most common pathogens causing nosocomial infections in humans and are of great concern for developing multidrug resistance. In the present study, K. pneumoniae and K. oxytoca from clinical samples were evaluated for their antibiotic sensitivity patterns against commonly used antibiotics and production of extended-spectrum beta-lactamase (ESBL). Materials and Methods: The isolates were obtained from tracheal swabs, sputum, wound swabs, pus, blood and urine samples of hospitalized patients. Klebsiella pneumoniae and Klebsiella oxytoca were identified by cultural and biochemical methods. Antibiotic sensitivity test was performed by modified Kirby-Bauer disc diffusion technique. ESBL production in Klebsiella spp. was confirmed by double disc synergy test. Results and Conclusion: Out of 500 clinical isolates, 120 were found positive for Klebsiella among which 108 were K. pneumoniae and 12 were K. oxytoca based on indole test. Prevalence rate of Klebsiella was found more prominent in males aged over 50 years, mostly in urine samples. Overall resistance pattern of Klebsiella isolates to ampicillin,amoxicillin, ceftriaxone, ciprofloxacin, co-trimoxazole, gentamicin, nalidixic acid,ttracycline was 100%, 90%, 45%, 40%, 45%, 25%, 50%, 35% respectively. Multidrug resistance was found more common in K. pneumoniae (56%) than in K. oxytoca (50%). Prevalence rate of ESBL producing Klebsiella was found 45% among which K. pneumoniae (50%) were found more prominent than K. oxytoca (25%). All the ESBL producing Klebsiella isolates were found to be multidrug resistant, showing 100% resistance to Ampicillin,Amoxicillin,Ceftriaxone and Ciprofloxacin.
format	Article
author	Sourav, Chakrabarty Mohsina, Kaniz Kumar Sarker, Palash Alam, Md Zahangir Abdul Karim, Mohamed Ismail Abu Sayem, S.M
author_facet	Sourav, Chakrabarty Mohsina, Kaniz Kumar Sarker, Palash Alam, Md Zahangir Abdul Karim, Mohamed Ismail Abu Sayem, S.M
author_sort	Sourav, Chakrabarty
title	Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
title_short	Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
title_full	Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
title_fullStr	Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
title_full_unstemmed	Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
title_sort	prevalence,antibiotic susceptibility profiles and esbl production in klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients
publisher	Croatian Society of Natural Sciences
publishDate	2016
url	http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/ http://irep.iium.edu.my/55043/1/Paper%20Antiboitic%20Sayem.pdf http://irep.iium.edu.my/55043/7/55043_Prevalence%2Cantibiotic_SCOPUS.pdf http://irep.iium.edu.my/55043/8/55043_Prevalence%2Cantibiotic_SCOPUS_WOS.pdf
first_indexed	2023-09-18T21:17:49Z
last_indexed	2023-09-18T21:17:49Z
_version_	1777411680466108416

Prevalence,antibiotic susceptibility profiles and ESBL production in Klebsiella pneumoniae and klebsiella oxytoca among hospitalized patients

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