Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters

Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters

Introduction: Preanalytical variation as a result of sample storage conditions are common issues in a large scale study since the sample has to be kept for an extended duration at ultra low temperature (-70ºC) before analysis. However, ultra low freezers may not be available in some laboratories. So...

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Main Authors: Abdullah, Nor Zamzila, A.Talib, Norlelawati, Ku Zaifah, Norsidah, Muhammad, Naznin, Mohamed, Abdul Hadi, Haderi, Fairuzzaidah
Format: Conference or Workshop Item
Language:English
Published: International Islamic University Malaysia 2016
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/55188/
http://irep.iium.edu.my/55188/
http://irep.iium.edu.my/55188/1/Effect%20of%20serum%20stored%20at%20various%20temperature%20on%20selected%20biochemical%20parameters%20MRS%202016.pdf
id iium-55188
recordtype eprints
spelling iium-551882017-02-27T02:31:41Z http://irep.iium.edu.my/55188/ Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters Abdullah, Nor Zamzila A.Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Naznin Mohamed, Abdul Hadi Haderi, Fairuzzaidah RB Pathology Introduction: Preanalytical variation as a result of sample storage conditions are common issues in a large scale study since the sample has to be kept for an extended duration at ultra low temperature (-70ºC) before analysis. However, ultra low freezers may not be available in some laboratories. Sometimes, the laboratory test may need to be repeated on the previously frozen and thawed samples. The aim of this research was to study the effect of storage temperature and freeze-thaw cycles on some biochemical parameters in our laboratory. Methods: An experimental study was carried out on 50 volunteers. Serum were aliquoted and stored at 4ºC, -30ºC and -70ºC. The serum was analyzed for total cholesterol (TC), triglycerides (TG), high density-lipoprotein (HDL-C) and glucose after 3 months of storage. The effects of freeze-thaw cycles were also recorded. Results: No significant differences (p>0.05) were seen in the level of the biochemical parameters between the samples stored at -70ºC and at -30ºC. The levels of selected parameters were higher after second freeze-thaw cycles as compared to the first thaw. All parameters showed significant positive correlations between the samples stored at -70ºC and the samples stored at -30ºC and 4ºC as well as between the first and second freeze-thaw cycles in samples kept at both -70ºC and -30ºC. Conclusions: Our finding suggested that the level of TC, TG and glucose in sample stored for 3 months at -70ºC are comparable with the samples stored at -30ºC. Only one freeze-thaw cycle is acceptable. International Islamic University Malaysia 2016 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55188/1/Effect%20of%20serum%20stored%20at%20various%20temperature%20on%20selected%20biochemical%20parameters%20MRS%202016.pdf Abdullah, Nor Zamzila and A.Talib, Norlelawati and Ku Zaifah, Norsidah and Muhammad, Naznin and Mohamed, Abdul Hadi and Haderi, Fairuzzaidah (2016) Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-76.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RB Pathology
spellingShingle RB Pathology
Abdullah, Nor Zamzila
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Naznin
Mohamed, Abdul Hadi
Haderi, Fairuzzaidah
Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
description Introduction: Preanalytical variation as a result of sample storage conditions are common issues in a large scale study since the sample has to be kept for an extended duration at ultra low temperature (-70ºC) before analysis. However, ultra low freezers may not be available in some laboratories. Sometimes, the laboratory test may need to be repeated on the previously frozen and thawed samples. The aim of this research was to study the effect of storage temperature and freeze-thaw cycles on some biochemical parameters in our laboratory. Methods: An experimental study was carried out on 50 volunteers. Serum were aliquoted and stored at 4ºC, -30ºC and -70ºC. The serum was analyzed for total cholesterol (TC), triglycerides (TG), high density-lipoprotein (HDL-C) and glucose after 3 months of storage. The effects of freeze-thaw cycles were also recorded. Results: No significant differences (p>0.05) were seen in the level of the biochemical parameters between the samples stored at -70ºC and at -30ºC. The levels of selected parameters were higher after second freeze-thaw cycles as compared to the first thaw. All parameters showed significant positive correlations between the samples stored at -70ºC and the samples stored at -30ºC and 4ºC as well as between the first and second freeze-thaw cycles in samples kept at both -70ºC and -30ºC. Conclusions: Our finding suggested that the level of TC, TG and glucose in sample stored for 3 months at -70ºC are comparable with the samples stored at -30ºC. Only one freeze-thaw cycle is acceptable.
format Conference or Workshop Item
author Abdullah, Nor Zamzila
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Naznin
Mohamed, Abdul Hadi
Haderi, Fairuzzaidah
author_facet Abdullah, Nor Zamzila
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Naznin
Mohamed, Abdul Hadi
Haderi, Fairuzzaidah
author_sort Abdullah, Nor Zamzila
title Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
title_short Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
title_full Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
title_fullStr Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
title_full_unstemmed Effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
title_sort effects of serum stored at various temperature and freeze-thaw cycles on selected biochemical parameters
publisher International Islamic University Malaysia
publishDate 2016
url http://irep.iium.edu.my/55188/
http://irep.iium.edu.my/55188/
http://irep.iium.edu.my/55188/1/Effect%20of%20serum%20stored%20at%20various%20temperature%20on%20selected%20biochemical%20parameters%20MRS%202016.pdf
first_indexed 2023-09-18T21:18:01Z
last_indexed 2023-09-18T21:18:01Z
_version_ 1777411693359398912

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