Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia

It is important to assess IAQ characteristics and to identify possible microbial contaminants in schools indoor environment because children are more vulnerable to air pollutants as they inhale more air pollutants per kilograms of body weight. Objectives: This study is done to assess and to compare...

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Main Authors:	Arifin, Mohd Hizrri, Mohd Ghazi, Zati Nabilah, Zaidi, Nurul Amni, Mohd Nazri, Nur Shahidah, Zahaba, Maryam, Abdul Hadi, Hazrin, Sharif, Mohd Faez, Mohd Aris, Mohd Shukri
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/55235/ http://irep.iium.edu.my/55235/1/ICEOH2016.pdf

id	iium-55235
recordtype	eprints
spelling	iium-552352017-03-08T08:13:10Z http://irep.iium.edu.my/55235/ Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia Arifin, Mohd Hizrri Mohd Ghazi, Zati Nabilah Zaidi, Nurul Amni Mohd Nazri, Nur Shahidah Zahaba, Maryam Abdul Hadi, Hazrin Sharif, Mohd Faez Mohd Aris, Mohd Shukri QR Microbiology It is important to assess IAQ characteristics and to identify possible microbial contaminants in schools indoor environment because children are more vulnerable to air pollutants as they inhale more air pollutants per kilograms of body weight. Objectives: This study is done to assess and to compare the level of selected IAQ parameters and microbiological contaminants inside the classroom of schools in urban area and rural area during occupied and non-occupied period. This study also aims to identify airborne bacteria species and fungi genera within classroom of schools in those area. Methods: School X (SX) is located in Kuantan, Pahang, while school Y (SY) is located in Pekan, Pahang. The physical IAQ parameters (Temperature, Relative Humidity (RH), Carbon Dioxide (CO2)) and airborne particulate matter (PM) were measured using VelociCalc® Multi-Function Ventilation Meter 9565 (TSI®, Minnesota, USA), and DustMate (Turnkey Instruments, UK) respectively. Surface Air System Indoor Air Quality (SAS IAQ), (PBI International, Italy) was used to collect the microbial contaminants and subsequently CFU were counted. The data were recorded for 30 minutes for each time-slot for 8 hours during occupied and non-occupied period within selected classrooms. Bacteria identification was done using 16S rRNA gene sequence analysis and fungi were identified macroscopically through direct identification technique up to genus level. The results were compared to standard reference limit based on Industrial Code of Practice on Indoor Air Quality (ICOP, 2010) regulated by the Department of Occupational Safety and Health (DOSH, 2005). Results: This study found that temperature (SX, Occupied; 34.9±3.9, Non-Occupied; 32.8±0.7), (SY, Occupied; 30.7±0.2, Non-Occupied; 30.6±0.5), RH (SY, Occupied; 74.4±2.9, Non-Ocupied; 70.05±1.0) and bacterial CFU counts (SX, Occupied; 558±308), (SY; Occupied; 903±415, Non-Occupied; 1176±303) exceeded the standard limit regulated by DOSH. Number of gram-negative bacteria dominated over gram-positive bacteria in both settings. Bacillus sp. (B. atrophaeus, B. subtilis, B. pumilus, B. altitudinis,. B. tequilensis and B. aerophilus). were the most dominant species, followed by Staphylococcus sp. (S. warneri, S. sciuri, S. haemolyticus and S. gallinarum). The common fungal species isolated in both schools during occupied and non-occupied period were Aspergillus, Alternaria, Cladosporium, Penicillium and Mucor. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/55235/1/ICEOH2016.pdf Arifin, Mohd Hizrri and Mohd Ghazi, Zati Nabilah and Zaidi, Nurul Amni and Mohd Nazri, Nur Shahidah and Zahaba, Maryam and Abdul Hadi, Hazrin and Sharif, Mohd Faez and Mohd Aris, Mohd Shukri (2016) Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia. In: International Conference on Environmental & Occupational Health (ICEOH 2016), 11th-13th April 2016, Putrajaya. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Arifin, Mohd Hizrri Mohd Ghazi, Zati Nabilah Zaidi, Nurul Amni Mohd Nazri, Nur Shahidah Zahaba, Maryam Abdul Hadi, Hazrin Sharif, Mohd Faez Mohd Aris, Mohd Shukri Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
description	It is important to assess IAQ characteristics and to identify possible microbial contaminants in schools indoor environment because children are more vulnerable to air pollutants as they inhale more air pollutants per kilograms of body weight. Objectives: This study is done to assess and to compare the level of selected IAQ parameters and microbiological contaminants inside the classroom of schools in urban area and rural area during occupied and non-occupied period. This study also aims to identify airborne bacteria species and fungi genera within classroom of schools in those area. Methods: School X (SX) is located in Kuantan, Pahang, while school Y (SY) is located in Pekan, Pahang. The physical IAQ parameters (Temperature, Relative Humidity (RH), Carbon Dioxide (CO2)) and airborne particulate matter (PM) were measured using VelociCalc® Multi-Function Ventilation Meter 9565 (TSI®, Minnesota, USA), and DustMate (Turnkey Instruments, UK) respectively. Surface Air System Indoor Air Quality (SAS IAQ), (PBI International, Italy) was used to collect the microbial contaminants and subsequently CFU were counted. The data were recorded for 30 minutes for each time-slot for 8 hours during occupied and non-occupied period within selected classrooms. Bacteria identification was done using 16S rRNA gene sequence analysis and fungi were identified macroscopically through direct identification technique up to genus level. The results were compared to standard reference limit based on Industrial Code of Practice on Indoor Air Quality (ICOP, 2010) regulated by the Department of Occupational Safety and Health (DOSH, 2005). Results: This study found that temperature (SX, Occupied; 34.9±3.9, Non-Occupied; 32.8±0.7), (SY, Occupied; 30.7±0.2, Non-Occupied; 30.6±0.5), RH (SY, Occupied; 74.4±2.9, Non-Ocupied; 70.05±1.0) and bacterial CFU counts (SX, Occupied; 558±308), (SY; Occupied; 903±415, Non-Occupied; 1176±303) exceeded the standard limit regulated by DOSH. Number of gram-negative bacteria dominated over gram-positive bacteria in both settings. Bacillus sp. (B. atrophaeus, B. subtilis, B. pumilus, B. altitudinis,. B. tequilensis and B. aerophilus). were the most dominant species, followed by Staphylococcus sp. (S. warneri, S. sciuri, S. haemolyticus and S. gallinarum). The common fungal species isolated in both schools during occupied and non-occupied period were Aspergillus, Alternaria, Cladosporium, Penicillium and Mucor.
format	Conference or Workshop Item
author	Arifin, Mohd Hizrri Mohd Ghazi, Zati Nabilah Zaidi, Nurul Amni Mohd Nazri, Nur Shahidah Zahaba, Maryam Abdul Hadi, Hazrin Sharif, Mohd Faez Mohd Aris, Mohd Shukri
author_facet	Arifin, Mohd Hizrri Mohd Ghazi, Zati Nabilah Zaidi, Nurul Amni Mohd Nazri, Nur Shahidah Zahaba, Maryam Abdul Hadi, Hazrin Sharif, Mohd Faez Mohd Aris, Mohd Shukri
author_sort	Arifin, Mohd Hizrri
title	Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
title_short	Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
title_full	Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
title_fullStr	Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
title_full_unstemmed	Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia
title_sort	indoor air auality (iaq) characteristics and microbial identific ations in two selected schools in pahang, malaysia
publishDate	2016
url	http://irep.iium.edu.my/55235/ http://irep.iium.edu.my/55235/1/ICEOH2016.pdf
first_indexed	2023-09-18T21:18:06Z
last_indexed	2023-09-18T21:18:06Z
_version_	1777411698146148352

Indoor Air Auality (IAQ) characteristics and microbial identific ations in two selected schools in Pahang, Malaysia

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