Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia

Pahang, Malaysia are very famous of its Pangasius spp (Patin) aquaculture activity. Pangasius spp. has excellent potential for inland aquaculture system with its fast growing ability and good market value in European markets (Singh and Lakra, 2012).Pangasius spp. is one of the largest and most impor...

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Main Authors:	Mansor, Nur Nazifah, Abdullah, Siti Zahrah, Mohamad, Syamil, Sulaiman, Sofiyah Amiera
Format:	Conference or Workshop Item
Language:	English English
Published:	2016
Subjects:	QR Microbiology SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/55346/ http://irep.iium.edu.my/55346/11/APAC2016%20Surabaya%20Cover.pdf http://irep.iium.edu.my/55346/12/APAC2016%20Surabaya%20Molecular%20Paper.pdf

id	iium-55346
recordtype	eprints
spelling	iium-553462017-02-13T19:48:16Z http://irep.iium.edu.my/55346/ Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia Mansor, Nur Nazifah Abdullah, Siti Zahrah Mohamad, Syamil Sulaiman, Sofiyah Amiera QR Microbiology SH Aquaculture. Fisheries. Angling Pahang, Malaysia are very famous of its Pangasius spp (Patin) aquaculture activity. Pangasius spp. has excellent potential for inland aquaculture system with its fast growing ability and good market value in European markets (Singh and Lakra, 2012).Pangasius spp. is one of the largest and most important inland fisheries in the world which mainly contribute as a food source. The production of Pangasius spp. in Malaysia showed tenfold increased from 1,625.21 tonnes in 2000 to 10,891.51 tonnes in 2011. However, Pangasius culture has been reported to face disease problem causing almost 30% mortalities especially in Sungai Pahang and Sungai Kuantan due to multiple infections of bacteria and virus. The reported bacterial disease was Motile Aeromonas Septicemia (MAS) while virus is channel catfish virus (CCV). Thus, the aim of this study is to understand Aeromonas hydrophila that had cause MAS infection in Pangasius hypophthalmus at Pahang River. Bacteria were collected from liver, kidney and spleen of Pangasius hypophthalmus cultured in Pahang River Malaysia. Isolated bacteria were cultured onto Brain Heart Infusion (BHI) Agar and identified using Gram staining, biochemical tests and API systems. Aeromonas spp. isolates were then subjected to conventional Polymerase Chain Reaction (PCR) using 16s rRNA gene and ITS gene. The amplified genes were sent for DNA sequencing and the results were tested with BLASTn to search for any sequence similarity with other bacteria in the Genebank. Construction of phylogenetic tree was carried out using MEGA 6 software. From the DNA sequencing method, isolates obtained were confirmed as Aeromonas hydrophila. Neighbor-joining method of phylogenetic tree constructed also reveals that the genetic variation of the isolates is similar with Aeromonas hydrophila from China and India. Thus, this information will facilitate to trigger a better prevention and treatment against MAS. 2016-04-26 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55346/11/APAC2016%20Surabaya%20Cover.pdf application/pdf en http://irep.iium.edu.my/55346/12/APAC2016%20Surabaya%20Molecular%20Paper.pdf Mansor, Nur Nazifah and Abdullah, Siti Zahrah and Mohamad, Syamil and Sulaiman, Sofiyah Amiera (2016) Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia. In: Asian Pacific Aquaculture 2016, 26-29 April 2016, Surabaya, Indonesia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology SH Aquaculture. Fisheries. Angling
spellingShingle	QR Microbiology SH Aquaculture. Fisheries. Angling Mansor, Nur Nazifah Abdullah, Siti Zahrah Mohamad, Syamil Sulaiman, Sofiyah Amiera Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
description	Pahang, Malaysia are very famous of its Pangasius spp (Patin) aquaculture activity. Pangasius spp. has excellent potential for inland aquaculture system with its fast growing ability and good market value in European markets (Singh and Lakra, 2012).Pangasius spp. is one of the largest and most important inland fisheries in the world which mainly contribute as a food source. The production of Pangasius spp. in Malaysia showed tenfold increased from 1,625.21 tonnes in 2000 to 10,891.51 tonnes in 2011. However, Pangasius culture has been reported to face disease problem causing almost 30% mortalities especially in Sungai Pahang and Sungai Kuantan due to multiple infections of bacteria and virus. The reported bacterial disease was Motile Aeromonas Septicemia (MAS) while virus is channel catfish virus (CCV). Thus, the aim of this study is to understand Aeromonas hydrophila that had cause MAS infection in Pangasius hypophthalmus at Pahang River. Bacteria were collected from liver, kidney and spleen of Pangasius hypophthalmus cultured in Pahang River Malaysia. Isolated bacteria were cultured onto Brain Heart Infusion (BHI) Agar and identified using Gram staining, biochemical tests and API systems. Aeromonas spp. isolates were then subjected to conventional Polymerase Chain Reaction (PCR) using 16s rRNA gene and ITS gene. The amplified genes were sent for DNA sequencing and the results were tested with BLASTn to search for any sequence similarity with other bacteria in the Genebank. Construction of phylogenetic tree was carried out using MEGA 6 software. From the DNA sequencing method, isolates obtained were confirmed as Aeromonas hydrophila. Neighbor-joining method of phylogenetic tree constructed also reveals that the genetic variation of the isolates is similar with Aeromonas hydrophila from China and India. Thus, this information will facilitate to trigger a better prevention and treatment against MAS.
format	Conference or Workshop Item
author	Mansor, Nur Nazifah Abdullah, Siti Zahrah Mohamad, Syamil Sulaiman, Sofiyah Amiera
author_facet	Mansor, Nur Nazifah Abdullah, Siti Zahrah Mohamad, Syamil Sulaiman, Sofiyah Amiera
author_sort	Mansor, Nur Nazifah
title	Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
title_short	Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
title_full	Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
title_fullStr	Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
title_full_unstemmed	Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia
title_sort	molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in pahang river, malaysia
publishDate	2016
url	http://irep.iium.edu.my/55346/ http://irep.iium.edu.my/55346/11/APAC2016%20Surabaya%20Cover.pdf http://irep.iium.edu.my/55346/12/APAC2016%20Surabaya%20Molecular%20Paper.pdf
first_indexed	2023-09-18T21:18:13Z
last_indexed	2023-09-18T21:18:13Z
_version_	1777411706244300800

Molecular characterization of aeromonas hydrophila isolated from pangasius hypophthalmus (sauvage, 1878) in Pahang river, Malaysia

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