Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction
The presence of pharmaceuticals in the environment had become a cause for snowballing concern in recent years. A wide range of pharmaceuticals had been discovered in fresh and marine waters and it had recently been shown that even in small quantities these compounds have the...
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2016
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| Online Access: | http://irep.iium.edu.my/55470/ http://irep.iium.edu.my/55470/1/PRC-2016.pdf |
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iium-554702017-03-02T02:15:24Z http://irep.iium.edu.my/55470/ Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction Helaluddin, Abul Bashar Mohammed Alaama, Mohammed Kamarbashrudin, Siti Khadijah Rose Izat, I'zma QD Chemistry R Medicine (General) RS Pharmacy and materia medica The presence of pharmaceuticals in the environment had become a cause for snowballing concern in recent years. A wide range of pharmaceuticals had been discovered in fresh and marine waters and it had recently been shown that even in small quantities these compounds have the potential to cause detriment to aquatic life. Being ranked as number one in the Top 50 Drugs by Expenditures in the Malaysian Statistics on Medicine, amlodipine might also contribute to the emerging issue of pharmaceutical wastewater pollution. This research project is designed to develop a methodology in detecting amlodipine analysis in pharmaceutical wastewater. Cetrizine was used as internal standard and samples were analysed using Agilent 1200 Series RP- HPLC equipped with quaternary pump and auto injector. Samples were injected to C18 column and the mobile phase consisted of ammonium acetate buffer (pH=4 adjusted using glacial acetic acid) and acetonitrile in the ratio 60- 40. The flow rate was 1 ml/min and UV detector was used for the detection and the wavelength was 248nm. Solid phase extraction (SPE) is used in this study for the extraction of amlodipine and IS. The recovery of amlodipine was evaluated over three QC concentrations and the recovery was in the range of 65%-72%. The method was linear over the range of 30-5000 ng/ml of amlodipine in wastewater. In general, the method can be used for the analysis of amlodipine in wastewater. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/55470/1/PRC-2016.pdf Helaluddin, Abul Bashar Mohammed and Alaama, Mohammed and Kamarbashrudin, Siti Khadijah Rose and Izat, I'zma (2016) Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction. In: 3rd Pharmaceutical Research Conference (3rd PRC 2016) for Pharmacy Students & Young Graduates, 28th-29th May 2016, Cyberjaya. (Unpublished) |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry R Medicine (General) RS Pharmacy and materia medica |
| spellingShingle |
QD Chemistry R Medicine (General) RS Pharmacy and materia medica Helaluddin, Abul Bashar Mohammed Alaama, Mohammed Kamarbashrudin, Siti Khadijah Rose Izat, I'zma Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| description |
The presence of pharmaceuticals in the environment had become a cause for
snowballing concern in recent years. A wide range of pharmaceuticals had
been discovered in fresh and marine waters and it had recently been shown
that even in small quantities these compounds have the potential to cause
detriment to aquatic life. Being ranked as number one in the Top 50 Drugs by
Expenditures in the Malaysian Statistics on Medicine, amlodipine might also
contribute to the emerging issue of pharmaceutical wastewater pollution. This
research project is designed to develop a methodology in detecting
amlodipine analysis in pharmaceutical wastewater. Cetrizine was used as
internal standard and samples were analysed using Agilent 1200 Series RP-
HPLC equipped with quaternary pump and auto injector. Samples were
injected to C18 column and the mobile phase consisted of ammonium acetate
buffer (pH=4 adjusted using glacial acetic acid) and acetonitrile in the ratio 60-
40. The flow rate was 1 ml/min and UV detector was used for the detection
and the wavelength was 248nm. Solid phase extraction (SPE) is used in this
study for the extraction of amlodipine and IS. The recovery of amlodipine was
evaluated over three QC concentrations and the recovery was in the range of
65%-72%. The method was linear over the range of 30-5000 ng/ml of
amlodipine in wastewater. In general, the method can be used for the analysis
of amlodipine in wastewater. |
| format |
Conference or Workshop Item |
| author |
Helaluddin, Abul Bashar Mohammed Alaama, Mohammed Kamarbashrudin, Siti Khadijah Rose Izat, I'zma |
| author_facet |
Helaluddin, Abul Bashar Mohammed Alaama, Mohammed Kamarbashrudin, Siti Khadijah Rose Izat, I'zma |
| author_sort |
Helaluddin, Abul Bashar Mohammed |
| title |
Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| title_short |
Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| title_full |
Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| title_fullStr |
Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| title_full_unstemmed |
Development and validation of new RP-HPLC method for the determination of amlodipine in pharmaceutical wastewater using SPE for extraction |
| title_sort |
development and validation of new rp-hplc method for the determination of amlodipine in pharmaceutical wastewater using spe for extraction |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/55470/ http://irep.iium.edu.my/55470/1/PRC-2016.pdf |
| first_indexed |
2023-09-18T21:18:24Z |
| last_indexed |
2023-09-18T21:18:24Z |
| _version_ |
1777411716850647040 |