YB-1 gene expression in A375 malignant melanoma cells

Background: YB-1 is a DNA and RNA binding protein that is involved in almost all DNA and mRNA related processes in cellular proliferation and differentiation. An increased expression of YB-1 is frequently being detected in different types of human cancers including breast, ovarian, thyroid and color...

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Main Authors:	Ibrahim, Wisam Nabeel, Abdul Wahab, Ridhwan, Ibrahim, Muhammad, Abdull Rasad, Mohammad Syaiful Bahari
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/55598/ http://irep.iium.edu.my/55598/1/55598_YB-1%20gene%20expression%20in%20A375.pdf

id	iium-55598
recordtype	eprints
spelling	iium-555982017-03-21T07:38:57Z http://irep.iium.edu.my/55598/ YB-1 gene expression in A375 malignant melanoma cells Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Ibrahim, Muhammad Abdull Rasad, Mohammad Syaiful Bahari Q Science (General) Background: YB-1 is a DNA and RNA binding protein that is involved in almost all DNA and mRNA related processes in cellular proliferation and differentiation. An increased expression of YB-1 is frequently being detected in different types of human cancers including breast, ovarian, thyroid and colorectal cancers. In addition, the elevated levels of YB-1 were found to be correlated with tumour progression and resistance to chemotherapy. Objectives/Research Problem: This study was conducted to measure YB-1 gene expression in A375 Malignant melanoma cell line by comparing its level with normal human fibroblasts cells. Materials and Method: These cell lines were derived from the American Type Cell Collection (ATCC), USA. The cells were then grown in Dulbecco’s Modified Eagle Medium supplied with 10% Foetal bovine serum, 1% of penicillin-streptomycin, 1% HEPES solution and 1% sodium pyruvate. After determination of the cell growth curve, the samples were collected for the exponential growth phase and the plateau growth phase. The samples RNA was then extracted and normalized for cDNA formation followed by reverse transcription PCR. The Cq values were determined for YB-1 protein, alpha tubulin and GAPDH. Results and Discussion: There was a 12.64-fold increase of YB-1 gene expression in the A375 cell line in comparison with normal fibroblasts cells; also, there was significant reduction in YB-1 gene expression in the plateau phase of growth in comparison with the exponential growth phase in the A375 cells (10.8-fold reduction in expression). 2016-11-21 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/55598/1/55598_YB-1%20gene%20expression%20in%20A375.pdf Ibrahim, Wisam Nabeel and Abdul Wahab, Ridhwan and Ibrahim, Muhammad and Abdull Rasad, Mohammad Syaiful Bahari (2016) YB-1 gene expression in A375 malignant melanoma cells. In: 1st Allied Health Scientific Colloqium (AHSC 2016), 21st-25th Nov. 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Ibrahim, Muhammad Abdull Rasad, Mohammad Syaiful Bahari YB-1 gene expression in A375 malignant melanoma cells
description	Background: YB-1 is a DNA and RNA binding protein that is involved in almost all DNA and mRNA related processes in cellular proliferation and differentiation. An increased expression of YB-1 is frequently being detected in different types of human cancers including breast, ovarian, thyroid and colorectal cancers. In addition, the elevated levels of YB-1 were found to be correlated with tumour progression and resistance to chemotherapy. Objectives/Research Problem: This study was conducted to measure YB-1 gene expression in A375 Malignant melanoma cell line by comparing its level with normal human fibroblasts cells. Materials and Method: These cell lines were derived from the American Type Cell Collection (ATCC), USA. The cells were then grown in Dulbecco’s Modified Eagle Medium supplied with 10% Foetal bovine serum, 1% of penicillin-streptomycin, 1% HEPES solution and 1% sodium pyruvate. After determination of the cell growth curve, the samples were collected for the exponential growth phase and the plateau growth phase. The samples RNA was then extracted and normalized for cDNA formation followed by reverse transcription PCR. The Cq values were determined for YB-1 protein, alpha tubulin and GAPDH. Results and Discussion: There was a 12.64-fold increase of YB-1 gene expression in the A375 cell line in comparison with normal fibroblasts cells; also, there was significant reduction in YB-1 gene expression in the plateau phase of growth in comparison with the exponential growth phase in the A375 cells (10.8-fold reduction in expression).
format	Conference or Workshop Item
author	Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Ibrahim, Muhammad Abdull Rasad, Mohammad Syaiful Bahari
author_facet	Ibrahim, Wisam Nabeel Abdul Wahab, Ridhwan Ibrahim, Muhammad Abdull Rasad, Mohammad Syaiful Bahari
author_sort	Ibrahim, Wisam Nabeel
title	YB-1 gene expression in A375 malignant melanoma cells
title_short	YB-1 gene expression in A375 malignant melanoma cells
title_full	YB-1 gene expression in A375 malignant melanoma cells
title_fullStr	YB-1 gene expression in A375 malignant melanoma cells
title_full_unstemmed	YB-1 gene expression in A375 malignant melanoma cells
title_sort	yb-1 gene expression in a375 malignant melanoma cells
publishDate	2016
url	http://irep.iium.edu.my/55598/ http://irep.iium.edu.my/55598/1/55598_YB-1%20gene%20expression%20in%20A375.pdf
first_indexed	2023-09-18T21:18:34Z
last_indexed	2023-09-18T21:18:34Z
_version_	1777411727684534272

YB-1 gene expression in A375 malignant melanoma cells

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