Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro

Matrix metalloproteinases (MMPs) comprise of a family of secreted and membrane-bound endopeptidases that hydrolyze extracellular matrix proteins. MMPs are one of many proteins which are undergoing scientific analysis in order to discover their therapeutic potentials. Collagenase 3 (MMP-13), a typ...

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Main Authors:	Mat Alewi, Nur Aizura, Abdull Rasad, Mohammad Syaiful Bahari
Format:	Conference or Workshop Item
Language:	English English
Published:	International Association of Computer Science & Information Technology Press 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/55641/ http://irep.iium.edu.my/55641/ http://irep.iium.edu.my/55641/1/12%20IPCBEE%20Characterization%20of%20Collagenase%203_.pdf http://irep.iium.edu.my/55641/2/55641.pdf

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recordtype	eprints
spelling	iium-556412017-04-13T02:13:15Z http://irep.iium.edu.my/55641/ Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro Mat Alewi, Nur Aizura Abdull Rasad, Mohammad Syaiful Bahari Q Science (General) Matrix metalloproteinases (MMPs) comprise of a family of secreted and membrane-bound endopeptidases that hydrolyze extracellular matrix proteins. MMPs are one of many proteins which are undergoing scientific analysis in order to discover their therapeutic potentials. Collagenase 3 (MMP-13), a type of collagenase from the MMP family, has been previously reported to be involved in the development and metastasis of cancers. However, the depth of its involvement in the underlying molecular mechanisms of cancer remains to be poorly known. This study aims to determine the degree of MMP-13 protein expressions in several human cancer cell lines and to ascertain its probable involvement in cancer development. From the study, it was hypothesized that the expression of MMP-13 are varied in several human cancer cell lines (MCF-7, HeLa and A375) and that its expression plays an important role in cancer progression and thus, by understanding such interactions, targeted therapeutic treatments could be developed over time. The quantitative expressions of MMP-13 in skin melanoma (A375), breast cancer (MCF-7) and cervix cancer (HeLa) cells were evaluated through ELISA test. The results showed a marked expression of MMP-13 proteins in MCF-7 (12900 pg/ml), followed by HeLa (8109 pg/ml) and A375 (7515 pg/ml). A375 showed the least amount of expression, which is supported by the fact that only certain forms of skin cancer have been found to express a significant amount of MMP-13. Overall, although variations were found in the expression of the collagenase in various human cancer cell lines, the results depict a prominent presence and probable involvement of MMP-13 in cancer. International Association of Computer Science & Information Technology Press 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55641/1/12%20IPCBEE%20Characterization%20of%20Collagenase%203_.pdf application/pdf en http://irep.iium.edu.my/55641/2/55641.pdf Mat Alewi, Nur Aizura and Abdull Rasad, Mohammad Syaiful Bahari (2013) Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro. In: 2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013),, 8th-9th June 2013, Kuala Lumpur, Malaysia. http://www.ipcbee.com/vol51/021-ICEEB2013-B10012.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mat Alewi, Nur Aizura Abdull Rasad, Mohammad Syaiful Bahari Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
description	Matrix metalloproteinases (MMPs) comprise of a family of secreted and membrane-bound endopeptidases that hydrolyze extracellular matrix proteins. MMPs are one of many proteins which are undergoing scientific analysis in order to discover their therapeutic potentials. Collagenase 3 (MMP-13), a type of collagenase from the MMP family, has been previously reported to be involved in the development and metastasis of cancers. However, the depth of its involvement in the underlying molecular mechanisms of cancer remains to be poorly known. This study aims to determine the degree of MMP-13 protein expressions in several human cancer cell lines and to ascertain its probable involvement in cancer development. From the study, it was hypothesized that the expression of MMP-13 are varied in several human cancer cell lines (MCF-7, HeLa and A375) and that its expression plays an important role in cancer progression and thus, by understanding such interactions, targeted therapeutic treatments could be developed over time. The quantitative expressions of MMP-13 in skin melanoma (A375), breast cancer (MCF-7) and cervix cancer (HeLa) cells were evaluated through ELISA test. The results showed a marked expression of MMP-13 proteins in MCF-7 (12900 pg/ml), followed by HeLa (8109 pg/ml) and A375 (7515 pg/ml). A375 showed the least amount of expression, which is supported by the fact that only certain forms of skin cancer have been found to express a significant amount of MMP-13. Overall, although variations were found in the expression of the collagenase in various human cancer cell lines, the results depict a prominent presence and probable involvement of MMP-13 in cancer.
format	Conference or Workshop Item
author	Mat Alewi, Nur Aizura Abdull Rasad, Mohammad Syaiful Bahari
author_facet	Mat Alewi, Nur Aizura Abdull Rasad, Mohammad Syaiful Bahari
author_sort	Mat Alewi, Nur Aizura
title	Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
title_short	Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
title_full	Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
title_fullStr	Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
title_full_unstemmed	Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
title_sort	characterization of collagenase 3 (mmp-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro
publisher	International Association of Computer Science & Information Technology Press
publishDate	2013
url	http://irep.iium.edu.my/55641/ http://irep.iium.edu.my/55641/ http://irep.iium.edu.my/55641/1/12%20IPCBEE%20Characterization%20of%20Collagenase%203_.pdf http://irep.iium.edu.my/55641/2/55641.pdf
first_indexed	2023-09-18T21:18:36Z
last_indexed	2023-09-18T21:18:36Z
_version_	1777411730212651008

Characterization of collagenase 3 (MMP-13) expressions in skin melanoma, breast cancer and cervical cancer in vitro

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