CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet

The primary human hypertrophic scar and normal skin fibroblasts were successfully established and identified by heat shock protein 47 and fibroblast surface protein markers. However, different patterns of protein expressions were found in cultured fibroblasts from hypertrophic scar and in normal fib...

Full description

Bibliographic Details
Main Authors:	Abdull Rasad, Mohammad Syaiful Bahari, Halim, Ahmad Sukari, Hashim, Kamaruddin, Abdul Rashid, Ahmad Hazri, Lim, Chin Keong, Shamsuddin, Shaharum
Format:	Article
Language:	English
Published:	Elsevier 2008
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/1/18%20C%20Journal%20of%20Biotech.pdf

id	iium-55645
recordtype	eprints
spelling	iium-556452017-04-13T01:30:22Z http://irep.iium.edu.my/55645/ CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid, Ahmad Hazri Lim, Chin Keong Shamsuddin, Shaharum Q Science (General) The primary human hypertrophic scar and normal skin fibroblasts were successfully established and identified by heat shock protein 47 and fibroblast surface protein markers. However, different patterns of protein expressions were found in cultured fibroblasts from hypertrophic scar and in normal fibroblast skin cells after treatment with the chitosan derivatives sheet. The CTCF protein was up-regulated in fibroblast hypertrophic scar on treatment with chitosan derivatives. In contrast, the amount of CTCF protein was found unchanged in normal skin fibroblasts both treated and untreated. The YB-1 protein was expressed almost similarly in normal and hypertrophic scar when treated with chitosan but the expression differed when untreated. The c-myc and p53 proteins were expressed in fibroblast hypertrophic scar followed by up-regulation after treatment with chitosan derivatives. The c-myc and p53 expressions were not detected in normal fibroblasts neither untreated nor treated. The CTCF, YB-1, c-myc, and p53 proteins acted in different manners in human hypertrophic scar and normal fibroblast skin cells. The novel chitosan derivatives sheet in this study may play roles in the control of cell growth and proliferation of human hypertrophic scar and normal fibroblast skin cells. The mechanisms underlying expression of these protein factors remain unclear, and further studies are still undergoing in our laboratory. Elsevier 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55645/1/18%20C%20Journal%20of%20Biotech.pdf Abdull Rasad, Mohammad Syaiful Bahari and Halim, Ahmad Sukari and Hashim, Kamaruddin and Abdul Rashid, Ahmad Hazri and Lim, Chin Keong and Shamsuddin, Shaharum (2008) CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet. Chinese Journal of Biotechnology, 24 (2). pp. 2137-2139. ISSN 1000-3061 http://www.sciencedirect.com/science/article/pii/S1872207509600091 10.1016/S1872-2075(09)60009-1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid, Ahmad Hazri Lim, Chin Keong Shamsuddin, Shaharum CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
description	The primary human hypertrophic scar and normal skin fibroblasts were successfully established and identified by heat shock protein 47 and fibroblast surface protein markers. However, different patterns of protein expressions were found in cultured fibroblasts from hypertrophic scar and in normal fibroblast skin cells after treatment with the chitosan derivatives sheet. The CTCF protein was up-regulated in fibroblast hypertrophic scar on treatment with chitosan derivatives. In contrast, the amount of CTCF protein was found unchanged in normal skin fibroblasts both treated and untreated. The YB-1 protein was expressed almost similarly in normal and hypertrophic scar when treated with chitosan but the expression differed when untreated. The c-myc and p53 proteins were expressed in fibroblast hypertrophic scar followed by up-regulation after treatment with chitosan derivatives. The c-myc and p53 expressions were not detected in normal fibroblasts neither untreated nor treated. The CTCF, YB-1, c-myc, and p53 proteins acted in different manners in human hypertrophic scar and normal fibroblast skin cells. The novel chitosan derivatives sheet in this study may play roles in the control of cell growth and proliferation of human hypertrophic scar and normal fibroblast skin cells. The mechanisms underlying expression of these protein factors remain unclear, and further studies are still undergoing in our laboratory.
format	Article
author	Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid, Ahmad Hazri Lim, Chin Keong Shamsuddin, Shaharum
author_facet	Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid, Ahmad Hazri Lim, Chin Keong Shamsuddin, Shaharum
author_sort	Abdull Rasad, Mohammad Syaiful Bahari
title	CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
title_short	CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
title_full	CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
title_fullStr	CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
title_full_unstemmed	CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
title_sort	ctcf, yb-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet
publisher	Elsevier
publishDate	2008
url	http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/ http://irep.iium.edu.my/55645/1/18%20C%20Journal%20of%20Biotech.pdf
first_indexed	2023-09-18T21:18:37Z
last_indexed	2023-09-18T21:18:37Z
_version_	1777411730785173504

CTCF, YB-1, c-myc, and p53 expressions of primary human hypertrophic scar and normal fibroblast skin cells in response to novel chitosan derivatives sheet

Similar Items