Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol

The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two differe...

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Main Authors:	Tasnuva, S. T., Ferdosh, Sahena, Ahmed, Qamar Uddin, Kashif, G, Jafri, Juliana, Sarker, Md. Zaidul Islam
Format:	Article
Language:	English English English
Published:	National Institute of Science Communication and Information Resources (NISCAIR) 2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/55726/ http://irep.iium.edu.my/55726/ http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf

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recordtype	eprints
spelling	iium-557262017-07-03T03:35:48Z http://irep.iium.edu.my/55726/ Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol Tasnuva, S. T. Ferdosh, Sahena Ahmed, Qamar Uddin Kashif, G Jafri, Juliana Sarker, Md. Zaidul Islam Q Science (General) The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two different assays, antioxidant assay (determination of total phenolic content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl assay) and antidiabetic assay (inhibition of α-amylase and α-glucosidase). GC Q-TOF MS profiling for both extracts was done after derivetisation to confirm the presence of bioactive compounds. SFE was performed at 40 MPa pressure, 60 °C temperature and 5 mL/min CO2 flow rate using 30 % ethanol (co-solvent) for 2 h. EtOHconv prepared using 95 % ethanol through conventional method (maceration) showed a good in vitro antioxidant potential and digestive enzymes inhibitory effect compared to supercritical fluid extract. α-amylase and α-glucosidase inhibitory activities for EtOHconv at 1 mg/mL were 30.08 % (±5.23) and 38.29 % (±2.52), whereas for standard acarbose it was 28.24 % (±13.66) and 36.93 % (±2.70), respectively. Supercritical fluid extract showed less potent in vitro antioxidant and digestive enzymes inhibitory effects (15.67±4.03- α-amylase, 28.36±2.01- α-glucosidase). GC Q-TOF MS analysis was done to confirm the presence of bioactive compounds in both the extracts. Although EtOHconv showed better results, SFE was found to contain more bioactive compounds associated with various pharmacological effects especially antioxidative as per GC Q-TOF MS results. SFE being a clean and green technology could be employed in future with more focus on method development and optimization to reproduce better and safe bioactive products from the neglected weed M. pudica. National Institute of Science Communication and Information Resources (NISCAIR) 2017-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf application/pdf en http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf Tasnuva, S. T. and Ferdosh, Sahena and Ahmed, Qamar Uddin and Kashif, G and Jafri, Juliana and Sarker, Md. Zaidul Islam (2017) Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol. Indian Journal of Natural Products and Resources (IJNPR), 8 (1). pp. 54-62. ISSN 0976-0504 E-ISSN 0976-0512 http://nopr.niscair.res.in/bitstream/123456789/41452/1/IJNPR%208%281%29%2054-62.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	Q Science (General)
spellingShingle	Q Science (General) Tasnuva, S. T. Ferdosh, Sahena Ahmed, Qamar Uddin Kashif, G Jafri, Juliana Sarker, Md. Zaidul Islam Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
description	The present study compared conventional (maceration) extraction (EtOHconv) and supercritical fluid extraction (SFE) methods as a mean of comparing conventional and green process for a weed namely Mimosa pudica L. to obtain a safe antidiabetic natural agent. In vitro analysis comprised of two different assays, antioxidant assay (determination of total phenolic content, total flavonoid content, and 2,2-diphenyl-1-picrylhydrazyl assay) and antidiabetic assay (inhibition of α-amylase and α-glucosidase). GC Q-TOF MS profiling for both extracts was done after derivetisation to confirm the presence of bioactive compounds. SFE was performed at 40 MPa pressure, 60 °C temperature and 5 mL/min CO2 flow rate using 30 % ethanol (co-solvent) for 2 h. EtOHconv prepared using 95 % ethanol through conventional method (maceration) showed a good in vitro antioxidant potential and digestive enzymes inhibitory effect compared to supercritical fluid extract. α-amylase and α-glucosidase inhibitory activities for EtOHconv at 1 mg/mL were 30.08 % (±5.23) and 38.29 % (±2.52), whereas for standard acarbose it was 28.24 % (±13.66) and 36.93 % (±2.70), respectively. Supercritical fluid extract showed less potent in vitro antioxidant and digestive enzymes inhibitory effects (15.67±4.03- α-amylase, 28.36±2.01- α-glucosidase). GC Q-TOF MS analysis was done to confirm the presence of bioactive compounds in both the extracts. Although EtOHconv showed better results, SFE was found to contain more bioactive compounds associated with various pharmacological effects especially antioxidative as per GC Q-TOF MS results. SFE being a clean and green technology could be employed in future with more focus on method development and optimization to reproduce better and safe bioactive products from the neglected weed M. pudica.
format	Article
author	Tasnuva, S. T. Ferdosh, Sahena Ahmed, Qamar Uddin Kashif, G Jafri, Juliana Sarker, Md. Zaidul Islam
author_facet	Tasnuva, S. T. Ferdosh, Sahena Ahmed, Qamar Uddin Kashif, G Jafri, Juliana Sarker, Md. Zaidul Islam
author_sort	Tasnuva, S. T.
title	Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_short	Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_full	Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_fullStr	Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_full_unstemmed	Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol
title_sort	mimosa pudica l.: a comparative study via in vitro analysis and gc q-tof ms profiling on conventional and supercritical fluid extraction using food grade ethanol
publisher	National Institute of Science Communication and Information Resources (NISCAIR)
publishDate	2017
url	http://irep.iium.edu.my/55726/ http://irep.iium.edu.my/55726/ http://irep.iium.edu.my/55726/1/55726-Mimosa%20pudica.pdf http://irep.iium.edu.my/55726/2/55726-Mimosa%20pudica_SCOPUS.pdf http://irep.iium.edu.my/55726/3/55726-Mimosa%20pudica_WOS.pdf
first_indexed	2023-09-18T21:18:42Z
last_indexed	2023-09-18T21:18:42Z
_version_	1777411735889641472

Mimosa pudica L.: A comparative study via in vitro analysis and GC Q-TOF MS profiling on conventional and supercritical fluid extraction using food grade ethanol

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