Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol the extracts examined for their antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test IC50 was ranged from 55...
| Main Authors: | , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/1/55736_evaluation.pdf |
| Summary: | Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents
such as hexane, dichloromethane (DCM) and methanol the extracts examined for their
antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging
test IC50 was ranged from 55±0.89 to4851±0011μg/ml. In the β-carotene bleaching assay the
antioxidant activity was 90.02±1.51% for the positive control (Trolox) and 88.34±1.31% for
the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts
was determined using the Folin-Ciocalteuprocedure, methanol pulp part demonstrated the
highest phenol content value of 6570.74±1.14 mg GAE/ g of dry sample. While the total
flavonoid content was determined using the aluminium chloride colorimetric assay highest
value of 2545.01±0.91 mg QE/ g indicated by pulp part of the fruit methanol extract.
Theantimicrobial activity of the crude extracts was tested using disc diffusion method against
pathogenic microorganisms: S. aureus, S. epidermidis, B. cereus, S. typhimurium, E. coli, K.
pneumonia and C. albican. Methanol extract of pulp part was recorded to have the highest
zone of inhibition against Gram-positive and Gram-negative bacteria. The MIC and
MBC/MFC for the extracts were also determined using the microdilution method ranged
from 4000-63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to
4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and
phenolic content was established. The results shows that the various parts of A. altilis fruit
extracts promising antioxidant activities have a potential bioactivities due to high content of
phenolic compounds. |
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