Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts

Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol the extracts examined for their antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test IC50 was ranged from 55...

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Main Authors:	Abdul Wahab, Ridhwan, Jalal, Tara Kamal, Draman, Samsul, Md Isa, Muhammad Lokman, Abdull Rasad, Mohammad Syaiful Bahari, Omar, Muhammad Nor, Ibrahim, Muhammad
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/1/55736_evaluation.pdf

id	iium-55736
recordtype	eprints
spelling	iium-557362017-04-19T03:21:14Z http://irep.iium.edu.my/55736/ Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts Abdul Wahab, Ridhwan Jalal, Tara Kamal Draman, Samsul Md Isa, Muhammad Lokman Abdull Rasad, Mohammad Syaiful Bahari Omar, Muhammad Nor Ibrahim, Muhammad Q Science (General) Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol the extracts examined for their antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test IC50 was ranged from 55±0.89 to4851±0011μg/ml. In the β-carotene bleaching assay the antioxidant activity was 90.02±1.51% for the positive control (Trolox) and 88.34±1.31% for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteuprocedure, methanol pulp part demonstrated the highest phenol content value of 6570.74±1.14 mg GAE/ g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay highest value of 2545.01±0.91 mg QE/ g indicated by pulp part of the fruit methanol extract. Theantimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: S. aureus, S. epidermidis, B. cereus, S. typhimurium, E. coli, K. pneumonia and C. albican. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The MIC and MBC/MFC for the extracts were also determined using the microdilution method ranged from 4000-63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have a potential bioactivities due to high content of phenolic compounds. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/55736/1/55736_evaluation.pdf Abdul Wahab, Ridhwan and Jalal, Tara Kamal and Draman, Samsul and Md Isa, Muhammad Lokman and Abdull Rasad, Mohammad Syaiful Bahari and Omar, Muhammad Nor and Ibrahim, Muhammad (2014) Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. In: International Health Conference IIUM 2014, 3rd-4th Dec. 2014, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/ihci/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdul Wahab, Ridhwan Jalal, Tara Kamal Draman, Samsul Md Isa, Muhammad Lokman Abdull Rasad, Mohammad Syaiful Bahari Omar, Muhammad Nor Ibrahim, Muhammad Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
description	Artocarpusaltilis(breadfruit) pulp, peeland whole fruit were extracted with various solvents such as hexane, dichloromethane (DCM) and methanol the extracts examined for their antioxidant activity usingthe stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test IC50 was ranged from 55±0.89 to4851±0011μg/ml. In the β-carotene bleaching assay the antioxidant activity was 90.02±1.51% for the positive control (Trolox) and 88.34±1.31% for the pulp part of the fruit methanol extract. The total phenolic content of the crude extracts was determined using the Folin-Ciocalteuprocedure, methanol pulp part demonstrated the highest phenol content value of 6570.74±1.14 mg GAE/ g of dry sample. While the total flavonoid content was determined using the aluminium chloride colorimetric assay highest value of 2545.01±0.91 mg QE/ g indicated by pulp part of the fruit methanol extract. Theantimicrobial activity of the crude extracts was tested using disc diffusion method against pathogenic microorganisms: S. aureus, S. epidermidis, B. cereus, S. typhimurium, E. coli, K. pneumonia and C. albican. Methanol extract of pulp part was recorded to have the highest zone of inhibition against Gram-positive and Gram-negative bacteria. The MIC and MBC/MFC for the extracts were also determined using the microdilution method ranged from 4000-63 μg/ml against pathogenic microbes. The MBC/MFC values varied from 250 to 4000 μg/ml. A correlation between antioxidant activity assays, antimicrobial activity and phenolic content was established. The results shows that the various parts of A. altilis fruit extracts promising antioxidant activities have a potential bioactivities due to high content of phenolic compounds.
format	Conference or Workshop Item
author	Abdul Wahab, Ridhwan Jalal, Tara Kamal Draman, Samsul Md Isa, Muhammad Lokman Abdull Rasad, Mohammad Syaiful Bahari Omar, Muhammad Nor Ibrahim, Muhammad
author_facet	Abdul Wahab, Ridhwan Jalal, Tara Kamal Draman, Samsul Md Isa, Muhammad Lokman Abdull Rasad, Mohammad Syaiful Bahari Omar, Muhammad Nor Ibrahim, Muhammad
author_sort	Abdul Wahab, Ridhwan
title	Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
title_short	Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
title_full	Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
title_fullStr	Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
title_full_unstemmed	Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
title_sort	evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts
publishDate	2014
url	http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/ http://irep.iium.edu.my/55736/1/55736_evaluation.pdf
first_indexed	2023-09-18T21:18:43Z
last_indexed	2023-09-18T21:18:43Z
_version_	1777411736916197376

Evaluation of antioxidant, total phenolsand flavonoids content and antimicrobial activities of Artocarpus altilis (breadfruit) of underutilized tropical fruit extracts

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