Separation and purification of cellulase from fermentation broth of sewage sludge

In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce su...

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Main Authors:	Mukhtaruddin, Muna Tasnim, Alam, Md. Zahangir, Mohd. Salleh, Hamzah, Rashid , Shah Samiur
Format:	Conference or Workshop Item
Language:	English English
Published:	2010
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5585/ http://irep.iium.edu.my/5585/ http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf

id	iium-5585
recordtype	eprints
spelling	iium-55852013-06-25T01:37:17Z http://irep.iium.edu.my/5585/ Separation and purification of cellulase from fermentation broth of sewage sludge Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur TP Chemical technology TP248.13 Biotechnology In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the purification of cellulases produced from fermentation broth of sewage sludge. In this study, crossflow filteration was used for the purification method using microfiltration (0.45 μm) and utrafiltration (10kD, 5 kD and 1 kD). The results revealed that ultrafiltration with membrane size of 1kD gave the best result for purification of cellulase from fermentation broth of sewage sludge which increases the enzyme activity from 22 U/ml after microfiltration to 50 U/ml after 1 kD of ultrafiltration. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf application/pdf en http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf Mukhtaruddin, Muna Tasnim and Alam, Md. Zahangir and Mohd. Salleh, Hamzah and Rashid , Shah Samiur (2010) Separation and purification of cellulase from fermentation broth of sewage sludge. In: 2010 International Conference on Process Engineering and Advanced Materials (icpeam2010), 15th-17th June 2010, Kuala Lumpur Convention Centre, Kuala Lumpur. http://www.utp.edu.my/icpeam2010/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur Separation and purification of cellulase from fermentation broth of sewage sludge
description	In recent years, growing attention has been paid to the conversion of biomass into bioethanol which is considered the cleanest liquid fuel alternative to fossil fuels. One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the purification of cellulases produced from fermentation broth of sewage sludge. In this study, crossflow filteration was used for the purification method using microfiltration (0.45 μm) and utrafiltration (10kD, 5 kD and 1 kD). The results revealed that ultrafiltration with membrane size of 1kD gave the best result for purification of cellulase from fermentation broth of sewage sludge which increases the enzyme activity from 22 U/ml after microfiltration to 50 U/ml after 1 kD of ultrafiltration.
format	Conference or Workshop Item
author	Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur
author_facet	Mukhtaruddin, Muna Tasnim Alam, Md. Zahangir Mohd. Salleh, Hamzah Rashid , Shah Samiur
author_sort	Mukhtaruddin, Muna Tasnim
title	Separation and purification of cellulase from fermentation broth of sewage sludge
title_short	Separation and purification of cellulase from fermentation broth of sewage sludge
title_full	Separation and purification of cellulase from fermentation broth of sewage sludge
title_fullStr	Separation and purification of cellulase from fermentation broth of sewage sludge
title_full_unstemmed	Separation and purification of cellulase from fermentation broth of sewage sludge
title_sort	separation and purification of cellulase from fermentation broth of sewage sludge
publishDate	2010
url	http://irep.iium.edu.my/5585/ http://irep.iium.edu.my/5585/ http://irep.iium.edu.my/5585/4/Separation_and_Purification_of_Cellulase.pdf http://irep.iium.edu.my/5585/1/purification_of_cellulase-conference.pdf
first_indexed	2023-09-18T20:14:15Z
last_indexed	2023-09-18T20:14:15Z
_version_	1777407681860993024

Separation and purification of cellulase from fermentation broth of sewage sludge

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