Association of MMP-9 gene polymorphisms with nephrolithiasis patients
Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease (CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular matrix which correlate with the pathogenesis of atherosclerosis. The current study was designed to analyze the association of (R...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Wiley-Liss Inc.
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/8/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with_scopus.pdf http://irep.iium.edu.my/55862/15/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients_WoS.pdf http://irep.iium.edu.my/55862/20/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients.pdf |
| Summary: | Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease
(CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular
matrix which correlate with the pathogenesis of atherosclerosis. The current study
was designed to analyze the association of (R279Q, C1562T) polymorphism of MMP-9
with nephrolithiasis patients.
Methods: Genotyping of MMP-9/R279Q and of MMP-9/C1562T polymorphism
were carried out by PCR-based restriction digestion method. Serum level of MMP-9,
oxidative stress marker, MDA, and uric acid were measured in patients and control.
Results: Allele frequencies of the MMP-9/C1562T polymorphism for C and T allele
were 71.25% and 28.75% in patients, 87.08% and 12.92% in control respectively. The
homozygote TT was more frequent in the nephrolithiasis patients group, while T allele
frequency was significantly higher in the nephrolithiasis patients group than in the
control group. The patients with CT and TT genotype showed a significant increase in
serum MMP-9, Total Oxidant Status (TOS), Oxidative Stress Index (OSI),
Malondialdehyde (MDA), and uric acid when compared to CC genotype in patients
with nephrolithiasis. The R279Q polymorphism site with regard to the relationship
with nephrolithiasis was not significant.
Conclusion: The result indicates that patients with TT genotype had an increased risk
of stones. Also, the results demonstrate that TT allele of the C1562T polymorphism in
the MMP-9gene is related with an increase of oxidative stress in nephrolithiasis
patients and may possibly impose a risk for cardiovascular diseases in patients with TT
genotype of MMP-9. |
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