Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits

tPurpose:Pseudomonas aeruginosa is the most common causative organism for contact lens-associated corneal ulcer and is commonly treated with fluoroquinolones. With the emergence of resistant strains, it is important to investigate alternative therapies. Despite well-established efficacy of tazocin a...

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Main Authors:	Sujaya, Singh, Abdul Razak, Zulhilmi, Agarwal, Renu, Ngu, Robin, Nyein, Lwin L., Vasudevan, Sushil, Kapitonova, Marina, Kadir, Azida Juana, Visvaraja, Subrayan
Format:	Article
Language:	English English English
Published:	Mary Ann Liebert Inc. 2018
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/56342/ http://irep.iium.edu.my/56342/ http://irep.iium.edu.my/56342/2/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_SCOPUS.pdf http://irep.iium.edu.my/56342/3/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_WOS.pdf http://irep.iium.edu.my/56342/19/56342_Ocular%20permeation%20of%20topical%20tazocin.pdf

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spelling	iium-563422018-07-23T03:58:50Z http://irep.iium.edu.my/56342/ Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits Sujaya, Singh Abdul Razak, Zulhilmi Agarwal, Renu Ngu, Robin Nyein, Lwin L. Vasudevan, Sushil Kapitonova, Marina Kadir, Azida Juana Visvaraja, Subrayan RB Pathology tPurpose:Pseudomonas aeruginosa is the most common causative organism for contact lens-associated corneal ulcer and is commonly treated with fluoroquinolones. With the emergence of resistant strains, it is important to investigate alternative therapies. Despite well-established efficacy of tazocin against systemic Pseudomonas infections, its topical use for the treatment of Pseudomonas keratitis has not been described, hence this study was aimed to find the ocular permeation of Tazocin and its efficacy in treating keratitis in rabbit eyes.Methods: We investigated the ocular permeation of topical tazocin after single drop application in normal rabbit eyes by estimating piperacillin and tazobactam concentrations in cornea, aqueous, and vitreous using a validated LC-MS/MS method. Furthermore, we determined the efficacy of repeated dose administration of tazocin against experimentally induced P. aeruginosa keratitis in rabbits in comparison to moxifloxacin. To determine the efficacy, clinical examination, histopathological examination, and estimation of bacterial load and inflammatory cytokines in cornea were done.Results: Significant corneal concentration of piperacillin and tazobactam was detected in normal rabbit corneas after single dose treatment with tazocin. In rabbits with Pseudomonas-induced keratitis, topical tazocin caused significant clinical and histopathological improvement. This improvement was associated with reduction in corneal bacterial load and inflammatory cytokines. Compared to moxifloxacin 0.5%, tazocin treated group showed greater clinical response which was associated with higher interleukin (IL)-1β, lower tumor necrosis factor (TNF)-α, a comparable level of IL-8, greater reduction in corneal bacterial load, and lesser inflammatory cell infiltration.Conclusion: Tazocin showed good ocular penetration and was effective in treatment of Pseudomonas induced keratitis in rabbits. Mary Ann Liebert Inc. 2018-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56342/2/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/56342/3/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_WOS.pdf application/pdf en http://irep.iium.edu.my/56342/19/56342_Ocular%20permeation%20of%20topical%20tazocin.pdf Sujaya, Singh and Abdul Razak, Zulhilmi and Agarwal, Renu and Ngu, Robin and Nyein, Lwin L. and Vasudevan, Sushil and Kapitonova, Marina and Kadir, Azida Juana and Visvaraja, Subrayan (2018) Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits. Journal of Ocular Pharmacology and Therapeutics, 34 (1-2). pp. 214-223. ISSN 1080-7683 https://www.liebertpub.com/doi/abs/10.1089/jop.2017.0098?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RB Pathology
spellingShingle	RB Pathology Sujaya, Singh Abdul Razak, Zulhilmi Agarwal, Renu Ngu, Robin Nyein, Lwin L. Vasudevan, Sushil Kapitonova, Marina Kadir, Azida Juana Visvaraja, Subrayan Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
description	tPurpose:Pseudomonas aeruginosa is the most common causative organism for contact lens-associated corneal ulcer and is commonly treated with fluoroquinolones. With the emergence of resistant strains, it is important to investigate alternative therapies. Despite well-established efficacy of tazocin against systemic Pseudomonas infections, its topical use for the treatment of Pseudomonas keratitis has not been described, hence this study was aimed to find the ocular permeation of Tazocin and its efficacy in treating keratitis in rabbit eyes.Methods: We investigated the ocular permeation of topical tazocin after single drop application in normal rabbit eyes by estimating piperacillin and tazobactam concentrations in cornea, aqueous, and vitreous using a validated LC-MS/MS method. Furthermore, we determined the efficacy of repeated dose administration of tazocin against experimentally induced P. aeruginosa keratitis in rabbits in comparison to moxifloxacin. To determine the efficacy, clinical examination, histopathological examination, and estimation of bacterial load and inflammatory cytokines in cornea were done.Results: Significant corneal concentration of piperacillin and tazobactam was detected in normal rabbit corneas after single dose treatment with tazocin. In rabbits with Pseudomonas-induced keratitis, topical tazocin caused significant clinical and histopathological improvement. This improvement was associated with reduction in corneal bacterial load and inflammatory cytokines. Compared to moxifloxacin 0.5%, tazocin treated group showed greater clinical response which was associated with higher interleukin (IL)-1β, lower tumor necrosis factor (TNF)-α, a comparable level of IL-8, greater reduction in corneal bacterial load, and lesser inflammatory cell infiltration.Conclusion: Tazocin showed good ocular penetration and was effective in treatment of Pseudomonas induced keratitis in rabbits.
format	Article
author	Sujaya, Singh Abdul Razak, Zulhilmi Agarwal, Renu Ngu, Robin Nyein, Lwin L. Vasudevan, Sushil Kapitonova, Marina Kadir, Azida Juana Visvaraja, Subrayan
author_facet	Sujaya, Singh Abdul Razak, Zulhilmi Agarwal, Renu Ngu, Robin Nyein, Lwin L. Vasudevan, Sushil Kapitonova, Marina Kadir, Azida Juana Visvaraja, Subrayan
author_sort	Sujaya, Singh
title	Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
title_short	Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
title_full	Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
title_fullStr	Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
title_full_unstemmed	Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
title_sort	ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits
publisher	Mary Ann Liebert Inc.
publishDate	2018
url	http://irep.iium.edu.my/56342/ http://irep.iium.edu.my/56342/ http://irep.iium.edu.my/56342/2/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_SCOPUS.pdf http://irep.iium.edu.my/56342/3/56342_Ocular%20Permeation%20of%20Topical%20Tazocin_WOS.pdf http://irep.iium.edu.my/56342/19/56342_Ocular%20permeation%20of%20topical%20tazocin.pdf
first_indexed	2023-09-18T21:19:28Z
last_indexed	2023-09-18T21:19:28Z
_version_	1777411784407252992

Ocular permeation of topical tazocin and its effectiveness in the treatment of pseudomonas aeruginosa induced keratitis in rabbits

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