Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications

An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale p...

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Main Authors:	Mohamed Suffian, Izzat Fahimuddin, Garcia-Maya, Mitla, Brown, Paul, Bui, Tam, Nishimura, Yuya, Mohammad Johari Palermo, Amir Rafiq, Ogino, Chiaki, Kondo, Akihiko, Al-Jamal, Khuloud
Format:	Article
Language:	English English
Published:	Nature Publishing Group 2017
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/56547/ http://irep.iium.edu.my/56547/ http://irep.iium.edu.my/56547/1/index.html http://irep.iium.edu.my/56547/2/56547_yield%20optimization%20of%20hepatitis.pdf

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recordtype	eprints
spelling	iium-565472017-06-22T23:47:38Z http://irep.iium.edu.my/56547/ Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications Mohamed Suffian, Izzat Fahimuddin Garcia-Maya, Mitla Brown, Paul Bui, Tam Nishimura, Yuya Mohammad Johari Palermo, Amir Rafiq Ogino, Chiaki Kondo, Akihiko Al-Jamal, Khuloud RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively. The modified method produced ∼3-fold higher yield and greater purity of wild type HBc particles than the conventional method. Our results demonstrated that the modified method produce a better yield and purity of HBc particles in an E. coli-expression system, which are fully characterised and suitable to be used for drug delivery applications. Nature Publishing Group 2017-03-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56547/1/index.html application/pdf en http://irep.iium.edu.my/56547/2/56547_yield%20optimization%20of%20hepatitis.pdf Mohamed Suffian, Izzat Fahimuddin and Garcia-Maya, Mitla and Brown, Paul and Bui, Tam and Nishimura, Yuya and Mohammad Johari Palermo, Amir Rafiq and Ogino, Chiaki and Kondo, Akihiko and Al-Jamal, Khuloud (2017) Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications. Scientific Reports, 7. pp. 1-9. ISSN 2045-2322 http://dx.doi.org/10.1038/srep43160
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica Mohamed Suffian, Izzat Fahimuddin Garcia-Maya, Mitla Brown, Paul Bui, Tam Nishimura, Yuya Mohammad Johari Palermo, Amir Rafiq Ogino, Chiaki Kondo, Akihiko Al-Jamal, Khuloud Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
description	An E. coli expression system offers a mean for rapid, high yield and economical production of Hepatitis B Virus core (HBc) particles. However, high-level production of HBc particles in bacteria is demanding and optimisation of HBc particle yield from E. coli is required to improve laboratory-scale productivity for further drug delivery applications. Production steps involve bacterial culture, protein isolation, denaturation, purification and finally protein assembly. In this study, we describe a modified E. coli based method for purifying HBc particles and compare the results with those obtained using a conventional purification method. HBc particle morphology was confirmed by Atomic Force Microscopy (AFM). Protein specificity and secondary structure were confirmed by Western Blot and Circular Dichroism (CD), respectively. The modified method produced ∼3-fold higher yield and greater purity of wild type HBc particles than the conventional method. Our results demonstrated that the modified method produce a better yield and purity of HBc particles in an E. coli-expression system, which are fully characterised and suitable to be used for drug delivery applications.
format	Article
author	Mohamed Suffian, Izzat Fahimuddin Garcia-Maya, Mitla Brown, Paul Bui, Tam Nishimura, Yuya Mohammad Johari Palermo, Amir Rafiq Ogino, Chiaki Kondo, Akihiko Al-Jamal, Khuloud
author_facet	Mohamed Suffian, Izzat Fahimuddin Garcia-Maya, Mitla Brown, Paul Bui, Tam Nishimura, Yuya Mohammad Johari Palermo, Amir Rafiq Ogino, Chiaki Kondo, Akihiko Al-Jamal, Khuloud
author_sort	Mohamed Suffian, Izzat Fahimuddin
title	Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
title_short	Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
title_full	Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
title_fullStr	Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
title_full_unstemmed	Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications
title_sort	yield optimisation of hepatitis b virus core particles in e. coli expression system for drug delivery applications
publisher	Nature Publishing Group
publishDate	2017
url	http://irep.iium.edu.my/56547/ http://irep.iium.edu.my/56547/ http://irep.iium.edu.my/56547/1/index.html http://irep.iium.edu.my/56547/2/56547_yield%20optimization%20of%20hepatitis.pdf
first_indexed	2023-09-18T21:19:47Z
last_indexed	2023-09-18T21:19:47Z
_version_	1777411804793667584

Yield optimisation of hepatitis B virus core particles in e. coli expression system for drug delivery applications

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