Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility

The expression product of VASA gene is widely conserved germ line marker and participates in regulating the development of reproductive system and spermatogenesis in healthy man. Azoospermic is a condition which cannot produce sperm cells for reproduction activity. In Islam, seeking knowledge is an...

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Main Authors:	Md Isa, Muhammad Lokman, Mat Yusof, Afzan, Abdul Wahab, Azantee Yazmie, Ramli, Roszaman
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/56831/ http://irep.iium.edu.my/56831/ http://irep.iium.edu.my/56831/1/Conference%202WCII2016%205.pdf

id	iium-56831
recordtype	eprints
spelling	iium-568312017-10-15T17:01:41Z http://irep.iium.edu.my/56831/ Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility Md Isa, Muhammad Lokman Mat Yusof, Afzan Abdul Wahab, Azantee Yazmie Ramli, Roszaman Q Science (General) The expression product of VASA gene is widely conserved germ line marker and participates in regulating the development of reproductive system and spermatogenesis in healthy man. Azoospermic is a condition which cannot produce sperm cells for reproduction activity. In Islam, seeking knowledge is an obligatory to each Muslim in order to solve problems and for a better live. Thus to unveiled the problem of azoospermic in infertile man we attempt to determine the gene expression of VASA level in testes cells of non-obstructive azoospermic (NOA) sample. Samples were taken from three NOA patients by testicular sperm extraction (TESE) to obtain testicular biopsyies. Testicular cells were isolated and cultured in supplemented knockout DMEM media. VASA gene expression was determined by reverse transcriptase polymerase chain reaction (RT-PCR) for spermatogonial marker. No VASA expression was detected in spermatogonial-like stem cells culture on day 1, 7, 14 and 21. Our finding shown VASA gene was not expressed in vitro culture spermatogenesis might be associated with the abnormal differentiation of primordial germ cells that lead to male infertility. 2016-10-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56831/1/Conference%202WCII2016%205.pdf Md Isa, Muhammad Lokman and Mat Yusof, Afzan and Abdul Wahab, Azantee Yazmie and Ramli, Roszaman (2016) Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility. In: 2nd World Congress on Integration and Islamicisation: Focus on Medical and Health Care Sciences (2WCII 2016), 21st-23rd Oct. 2016, Kuantan, Pahang Darul Makmur. http://www.iium.edu.my/wcii/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Md Isa, Muhammad Lokman Mat Yusof, Afzan Abdul Wahab, Azantee Yazmie Ramli, Roszaman Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
description	The expression product of VASA gene is widely conserved germ line marker and participates in regulating the development of reproductive system and spermatogenesis in healthy man. Azoospermic is a condition which cannot produce sperm cells for reproduction activity. In Islam, seeking knowledge is an obligatory to each Muslim in order to solve problems and for a better live. Thus to unveiled the problem of azoospermic in infertile man we attempt to determine the gene expression of VASA level in testes cells of non-obstructive azoospermic (NOA) sample. Samples were taken from three NOA patients by testicular sperm extraction (TESE) to obtain testicular biopsyies. Testicular cells were isolated and cultured in supplemented knockout DMEM media. VASA gene expression was determined by reverse transcriptase polymerase chain reaction (RT-PCR) for spermatogonial marker. No VASA expression was detected in spermatogonial-like stem cells culture on day 1, 7, 14 and 21. Our finding shown VASA gene was not expressed in vitro culture spermatogenesis might be associated with the abnormal differentiation of primordial germ cells that lead to male infertility.
format	Conference or Workshop Item
author	Md Isa, Muhammad Lokman Mat Yusof, Afzan Abdul Wahab, Azantee Yazmie Ramli, Roszaman
author_facet	Md Isa, Muhammad Lokman Mat Yusof, Afzan Abdul Wahab, Azantee Yazmie Ramli, Roszaman
author_sort	Md Isa, Muhammad Lokman
title	Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
title_short	Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
title_full	Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
title_fullStr	Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
title_full_unstemmed	Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility
title_sort	identification of vasa gene expression in in-vitro culture from non-obstructive azoospermia (noa) testicular biopsy cells: a study to unlock knowledge of male infertility
publishDate	2016
url	http://irep.iium.edu.my/56831/ http://irep.iium.edu.my/56831/ http://irep.iium.edu.my/56831/1/Conference%202WCII2016%205.pdf
first_indexed	2023-09-18T21:20:14Z
last_indexed	2023-09-18T21:20:14Z
_version_	1777411832375410688

Identification of VASA gene expression in in-vitro culture from Non-Obstructive Azoospermia (NOA) testicular biopsy cells: a study to unlock knowledge of male infertility

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