Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity

Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics in abroad had been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is...

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Main Authors:	Abdul Wahab, Azantee Yazmie, Md Isa, Muhammad Lokman, Ramli, Roszaman, Mat Yusof, Afzan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/56832/ http://irep.iium.edu.my/56832/ http://irep.iium.edu.my/56832/1/Conference%202WCII2016%206.pdf

id	iium-56832
recordtype	eprints
spelling	iium-568322017-05-18T02:12:06Z http://irep.iium.edu.my/56832/ Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Mat Yusof, Afzan Q Science (General) Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics in abroad had been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is forbidden to use sample from male other than their spouse. It is according to maqasid syari’ah to ensure the heridity of the human being. Based on the latest technology, one approach of stem cell differentiation process had been established to produce mature cells from primitive or immature cells (stem cells). This technology is in line with the concept of maqasid syari’ah since we are using the cells from one person. Therefore, we try to adopt this technology to study a potential of testicular cells from non-obstructive azoospermic (NOA) patient to undergo in vitro spermatogenesis. Samples were cultured in modified human embryonic stem cells (HESC) media with specific growth factors; basic fibroblast growth factor (bFGF)and leukemia inhibitory factor (LIF). Protein expressions were analyzed by immunofluorescent staining on day 49 and 90 of culture. Results shown spermatogonial stem cells (SSCs) colonies formed after 14 to 21 days then the cells were successful expanding and stable in duration of 49 days. Then SSCs were differentiated into later stage of spermatogenesis on day 90. Four specifics SSCs protein markers were identified on day 49; ITGA1, ITGB1, CD9 and GFRA1 whereas SCP3 and TP1 proteins were expressed on day 90. This in vitro spermatogenesis suggested a closer approach for future clinical purposes for Muslim NOA patients in order to have their own children. 2016-10-21 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/56832/1/Conference%202WCII2016%206.pdf Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Ramli, Roszaman and Mat Yusof, Afzan (2016) Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity. In: 2nd World Congress on Integration and Islamicisation: Focus on Medical and Health Care Sciences (2WCII 2016), 21st-23rd Oct. 2016, Kuantan, Pahang Darul Makmur. http://www.iium.edu.my/wcii/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Mat Yusof, Afzan Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
description	Azoospermia is present in 15% of infertile cases and it is a major concern due to inability to produce sperm. Most of IVF (in-vitro fertilization) clinics in abroad had been using sperm donation via sperm bank facilities as a solution for infertile couple to have their own offspring. In Islam, it is forbidden to use sample from male other than their spouse. It is according to maqasid syari’ah to ensure the heridity of the human being. Based on the latest technology, one approach of stem cell differentiation process had been established to produce mature cells from primitive or immature cells (stem cells). This technology is in line with the concept of maqasid syari’ah since we are using the cells from one person. Therefore, we try to adopt this technology to study a potential of testicular cells from non-obstructive azoospermic (NOA) patient to undergo in vitro spermatogenesis. Samples were cultured in modified human embryonic stem cells (HESC) media with specific growth factors; basic fibroblast growth factor (bFGF)and leukemia inhibitory factor (LIF). Protein expressions were analyzed by immunofluorescent staining on day 49 and 90 of culture. Results shown spermatogonial stem cells (SSCs) colonies formed after 14 to 21 days then the cells were successful expanding and stable in duration of 49 days. Then SSCs were differentiated into later stage of spermatogenesis on day 90. Four specifics SSCs protein markers were identified on day 49; ITGA1, ITGB1, CD9 and GFRA1 whereas SCP3 and TP1 proteins were expressed on day 90. This in vitro spermatogenesis suggested a closer approach for future clinical purposes for Muslim NOA patients in order to have their own children.
format	Conference or Workshop Item
author	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Mat Yusof, Afzan
author_facet	Abdul Wahab, Azantee Yazmie Md Isa, Muhammad Lokman Ramli, Roszaman Mat Yusof, Afzan
author_sort	Abdul Wahab, Azantee Yazmie
title	Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
title_short	Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
title_full	Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
title_fullStr	Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
title_full_unstemmed	Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity
title_sort	spermatogonial stem cells protein marker identification from in-vitro differentiation of non-obstructive azoospermia (noa) testes biopsies cells: an approach in line with maqasid al shariah to maintain the heredity
publishDate	2016
url	http://irep.iium.edu.my/56832/ http://irep.iium.edu.my/56832/ http://irep.iium.edu.my/56832/1/Conference%202WCII2016%206.pdf
first_indexed	2023-09-18T21:20:14Z
last_indexed	2023-09-18T21:20:14Z
_version_	1777411832524308480

Spermatogonial stem cells protein marker identification from in-vitro differentiation of Non-Obstructive Azoospermia (NOA) testes biopsies cells: an approach in line with Maqasid al Shariah to maintain the heredity

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