Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study

Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high thr...

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Main Authors:	Sengupta, Pinaki, Chatterjee, Bappaditya, Mandal, Uttam Kumar, Gorain, Bapi, Pal, Tapan Kumar
Format:	Article
Language:	English English
Published:	Xi'an Jiaotong University 2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf

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spelling	iium-571042018-04-18T01:43:18Z http://irep.iium.edu.my/57104/ Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar RS Pharmacy and materia medica Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high throughput LC–MS/MS method for simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for bioanalytical method validation. A linear response of the analytes was observed over the range of 0.005–10 µg/mL with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%–106.10%. The intra- and inter-day precision ranged from 2.32% to 10.14% and 5.02% to 8.12%, respectively. The method was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical pharmacokinetic study. Due to the potential of PIO-TLM combination to be therapeutically explored, this method is expected to have significant usefulness in future. Xi'an Jiaotong University 2017-05-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf application/pdf en http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf Sengupta, Pinaki and Chatterjee, Bappaditya and Mandal, Uttam Kumar and Gorain, Bapi and Pal, Tapan Kumar (2017) Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study. Journal of Pharmaceutical Analysis, 7 (6). pp. 381-387. ISSN 2095-1779 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790748/pdf/main.pdf 10.1016/j.jpha.2017.05.004
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
description	Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high throughput LC–MS/MS method for simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for bioanalytical method validation. A linear response of the analytes was observed over the range of 0.005–10 µg/mL with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%–106.10%. The intra- and inter-day precision ranged from 2.32% to 10.14% and 5.02% to 8.12%, respectively. The method was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical pharmacokinetic study. Due to the potential of PIO-TLM combination to be therapeutically explored, this method is expected to have significant usefulness in future.
format	Article
author	Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar
author_facet	Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar
author_sort	Sengupta, Pinaki
title	Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
title_short	Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
title_full	Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
title_fullStr	Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
title_full_unstemmed	Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
title_sort	development and validation of a high throughput lc–ms/ms method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
publisher	Xi'an Jiaotong University
publishDate	2017
url	http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf
first_indexed	2023-09-18T21:20:40Z
last_indexed	2023-09-18T21:20:40Z
_version_	1777411859605880832

Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study

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