Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin

Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active natural quinolones of R. angustifolia and evaluating their potential as synergistic enhancers...

Full description

Bibliographic Details
Main Authors:	Mohd Kamal, Laina Zarisa, Mohd Hassan, Norazian, Md Taib, Nurhaya, Khin Soe, May
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/57344/ http://irep.iium.edu.my/57344/1/ICNP2017-NORAZIAN.pdf

id	iium-57344
recordtype	eprints
spelling	iium-573442017-11-08T01:47:18Z http://irep.iium.edu.my/57344/ Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya Khin Soe, May RS Pharmacy and materia medica Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active natural quinolones of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography-guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline], (III) as the major antimicrobial active alkaloid together with 2,3-dimethoxy-1-hydroxy-10-methylacridone [arborinine], (I) and 4,7,8-trimethoxyfuro [2,3-b]quinoline [skimmianine], (II). Alkaloid III and I showed Minimum Inhibitory Concentration (MIC) values ranging from 250 µg/ml to 1000 µg/ml against Staphylococcus aureus ATCC 25923, Enterococcus fecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between alkaloid III with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Index (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for III-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin indifference effect. Alkaloid III was a potential candidate for antimicrobial combination agent especially against S. aureus which supports the idea of using plant metabolites as antimicrobial synergistic agents. 2017-03-15 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/57344/1/ICNP2017-NORAZIAN.pdf Mohd Kamal, Laina Zarisa and Mohd Hassan, Norazian and Md Taib, Nurhaya and Khin Soe, May (2017) Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin. In: International Conference on Natural Products (ICNP 2017), 15th-16th March 2017, Lumut, Perak. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya Khin Soe, May Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
description	Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active natural quinolones of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography-guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline], (III) as the major antimicrobial active alkaloid together with 2,3-dimethoxy-1-hydroxy-10-methylacridone [arborinine], (I) and 4,7,8-trimethoxyfuro [2,3-b]quinoline [skimmianine], (II). Alkaloid III and I showed Minimum Inhibitory Concentration (MIC) values ranging from 250 µg/ml to 1000 µg/ml against Staphylococcus aureus ATCC 25923, Enterococcus fecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between alkaloid III with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Index (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for III-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin indifference effect. Alkaloid III was a potential candidate for antimicrobial combination agent especially against S. aureus which supports the idea of using plant metabolites as antimicrobial synergistic agents.
format	Conference or Workshop Item
author	Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya Khin Soe, May
author_facet	Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya Khin Soe, May
author_sort	Mohd Kamal, Laina Zarisa
title	Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
title_short	Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
title_full	Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
title_fullStr	Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
title_full_unstemmed	Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
title_sort	graveoline, an antimicrobial activ alkaloid from ruta angustifolia (l.) pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin
publishDate	2017
url	http://irep.iium.edu.my/57344/ http://irep.iium.edu.my/57344/1/ICNP2017-NORAZIAN.pdf
first_indexed	2023-09-18T21:21:03Z
last_indexed	2023-09-18T21:21:03Z
_version_	1777411883906629632

Graveoline, an antimicrobial activ alkaloid from Ruta angustifolia (L.) Pers. and its in vitro antimicrobial combination effects with either erythromycin or vancomycin

Similar Items