Effect of abiotic stress on carotenoids accumulation in pumpkin plants under light and dark conditions
In nature, environmental factors highly influence the carotenoid composition in pumpkin plants and these factors were difficult to control; thus, carotenoid content is varied quantitatively and qualitatively. However, certain parameters can be controlled and this can be conducted in the laborator...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English English |
| Published: |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
2017
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/57460/ http://irep.iium.edu.my/57460/ http://irep.iium.edu.my/57460/1/57460_Effect%20of%20abiotic%20stress%20on%20carotenoids.pdf http://irep.iium.edu.my/57460/7/57460_Effect%20of%20abiotic%20stress%20on%20carotenoids%20accumulation_scopus.pdf http://irep.iium.edu.my/57460/13/57460_Effect%20of%20abiotic%20stress%20on%20carotenoids_wos.pdf |
| Summary: | In nature, environmental factors highly influence the carotenoid composition in pumpkin plants
and these factors were difficult to control; thus, carotenoid content is varied quantitatively and
qualitatively. However, certain parameters can be controlled and this can be conducted in the
laboratory through biogenesis manipulation. This approach uses environmental stress as a tool
to alter the carotenoid pathway in the plants. The main objective of this study was to observe
the inhibiting and enhancing effect of abiotic stress on individual carotenoid accumulation in
pumpkin plants under light and dark conditions. The abiotic stresses used were plant elicitors
which consisted of Ultra Violet light exposure, Polyethylene Glycol 4000, Salicylic Acid,
and half strength nutrients using Murashige and Skoog Salt. After two weeks of treatments,
the pumpkin leaves and stems were harvested, freeze dried and extracted to determine the
carotenoids compound using High-Performance Liquid Chromatography (HPLC). Results
showed that there was a significant difference (p<0.005) of lutein content (2.668 + 0.565 µg/g)
in pumpkin leaves once exposed to Ultra Violet light with the absence of β-carotene compared
to the control treatment; lutein (3.119 ± 0.210 µg/g) and β-carotene (0.838 + 0.05 µg/g). There
were significant differences of carotenoids content under dark condition with the value of lutein
at 0.472 + 0.008 µg/g to 1.247 + 0.047 µg/g and β-carotene from not detected to 1.360 + 0.003.
The highest amount of lutein in pumpkin stem was detected in the Salicylic acid treatment
under light condition (0.930 + 0.101 µg/g) and the highest amount of β-carotene in the pumpkin
stem was detected in the Salicylic acid treatment under dark condition (0.234 + 0.018 µg/g).
The carotenoid content varied in each treatment due to the adaptation of pumpkin plants with
abiotic stress induced to them. |
|---|