Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics

Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water r...

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Main Authors:	Mediani, Ahmed, Abas, Faridah, Maulidiani, M., Khatib, Alfi, Tan, Chin Ping, Ismail, Intan Safinar, Shaari, Khozirah, Ismail, Amin
Format:	Article
Language:	English English
Published:	Multidisciplinary Digital Publishing Institute (MDPI) 2017
Subjects:	QD Chemistry QP Physiology RC Internal medicine
Online Access:	http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/1/57529_Characterization%20of%20metabolite%20profile_article.pdf http://irep.iium.edu.my/57529/2/57529_Characterization%20of%20metabolite%20profile_SCOPUS.pdf

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spelling	iium-575292018-04-16T06:26:35Z http://irep.iium.edu.my/57529/ Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Ismail, Intan Safinar Shaari, Khozirah Ismail, Amin QD Chemistry QP Physiology RC Internal medicine Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water ratios (0%, 50%, 70%, 80% and 100%) were evaluated for their metabolite changes using proton nuclear magnetic resonance (1H-NMR)-based metabolomics approach. The amino acids analysis showed that FD P. niruri exhibited higher content of most amino acids compared to the other dried samples. Based on principal component analysis (PCA), the FD P. niruri extracted with 80% ethanol contained higher amounts of hypophyllanthin and phenolic compounds based on the loading plot. The partial least-square (PLS) results showed that the phytochemicals, including hypophyllanthin, catechin, epicatechin, rutin, quercetin and chlorogenic, caffeic, malic and gallic acids were correlated with antioxidant and α-glucosidase inhibitory activities, which were higher in the FD material extracted with 80% ethanol. This report optimized the effect of drying and ethanol ratios and these findings demonstrate that NMR-based metabolomics was an applicable approach. The FD P. niruri extracted with 80% ethanol can be used as afunctional food ingredient for nutraceutical or in medicinal preparation. Multidisciplinary Digital Publishing Institute (MDPI) 2017-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57529/1/57529_Characterization%20of%20metabolite%20profile_article.pdf application/pdf en http://irep.iium.edu.my/57529/2/57529_Characterization%20of%20metabolite%20profile_SCOPUS.pdf Mediani, Ahmed and Abas, Faridah and Maulidiani, M. and Khatib, Alfi and Tan, Chin Ping and Ismail, Intan Safinar and Shaari, Khozirah and Ismail, Amin (2017) Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics. Molecules, 22 (6). pp. 1-14. ISSN 1420-3049 http://www.mdpi.com/1420-3049/22/6/902 10.3390/molecules22060902
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry QP Physiology RC Internal medicine
spellingShingle	QD Chemistry QP Physiology RC Internal medicine Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Ismail, Intan Safinar Shaari, Khozirah Ismail, Amin Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
description	Phyllanthus niruri is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried P. niruri samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water ratios (0%, 50%, 70%, 80% and 100%) were evaluated for their metabolite changes using proton nuclear magnetic resonance (1H-NMR)-based metabolomics approach. The amino acids analysis showed that FD P. niruri exhibited higher content of most amino acids compared to the other dried samples. Based on principal component analysis (PCA), the FD P. niruri extracted with 80% ethanol contained higher amounts of hypophyllanthin and phenolic compounds based on the loading plot. The partial least-square (PLS) results showed that the phytochemicals, including hypophyllanthin, catechin, epicatechin, rutin, quercetin and chlorogenic, caffeic, malic and gallic acids were correlated with antioxidant and α-glucosidase inhibitory activities, which were higher in the FD material extracted with 80% ethanol. This report optimized the effect of drying and ethanol ratios and these findings demonstrate that NMR-based metabolomics was an applicable approach. The FD P. niruri extracted with 80% ethanol can be used as afunctional food ingredient for nutraceutical or in medicinal preparation.
format	Article
author	Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Ismail, Intan Safinar Shaari, Khozirah Ismail, Amin
author_facet	Mediani, Ahmed Abas, Faridah Maulidiani, M. Khatib, Alfi Tan, Chin Ping Ismail, Intan Safinar Shaari, Khozirah Ismail, Amin
author_sort	Mediani, Ahmed
title	Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
title_short	Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
title_full	Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
title_fullStr	Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
title_full_unstemmed	Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
title_sort	characterization of metabolite profile in phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics
publisher	Multidisciplinary Digital Publishing Institute (MDPI)
publishDate	2017
url	http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/ http://irep.iium.edu.my/57529/1/57529_Characterization%20of%20metabolite%20profile_article.pdf http://irep.iium.edu.my/57529/2/57529_Characterization%20of%20metabolite%20profile_SCOPUS.pdf
first_indexed	2023-09-18T21:21:19Z
last_indexed	2023-09-18T21:21:19Z
_version_	1777411901347594240

Characterization of metabolite profile in Phyllanthus niruri and correlation with bioactivity elucidated by nuclear magnetic resonance based metabolomics

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