Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment
Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Be...
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Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM)
2017
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| Online Access: | http://irep.iium.edu.my/57590/ http://irep.iium.edu.my/57590/ http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf |
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iium-575902018-11-30T03:26:47Z http://irep.iium.edu.my/57590/ Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment Salih, Ghassan Ali Ahmad Raus, Raha Shaban, Mustaffa Nameer Abdullah, N. QD Chemistry QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) TP248.13 Biotechnology Breast cancer is considered as one of the most common cancers all over the world. A huge effort has been made to create a safe and cost effective breast cancer treatment. All of these features exist in the plants sources. In this study, the effect of local vegetable salad, Premna serratifolia (Bebuas) against MCF-7 cells (human breast adenocarcinoma) was determined. The optimum condition to extract breast cancer cytotoxic compound from the plant was investigated and the exact cytotoxic compound was identified as well. To determine the plant cytotoxicity effect against MCF-7 cells, MTT assay was used. Two important parameters in the sonication extraction method which are duration of time and temperature were optimized by carrying out a series of experiments which were designed by Face Centered Central Composite Design (FCCCD). The extraction efficiency of each experiment was determined by measuring the yield of extract and the half maximal inhibitory concentration (IC50) of the extract against MCF-7 cells. The results obtained from the experiments were fitted to the second order polynomial model to generate equation that was used to determine best extraction processing condition. Based on the generated equation, the best sonication processing condition to extract the cytotoxic compound is at 30o C for 67 min. Analysis of variance (ANOVA) showed that the duration of extraction time has great influence (p<0.05) on the yield of crude extract, while both parameters, time and temperature significantly (p<0.05) affected the IC50 value of the plant extract. Two purified compounds from the plant, acacetin and acacetin-7-O-glycoside were identified to be responsible for the cytotoxicity activity. Conclusively, in terms of pharmaceutical and medical field applications; the optimal extraction processing condition obtained from this study ensures that the extraction of cytotoxic compound from the plant is conducted at the low temperature and short duration of time. Further study on the identified compounds is required as it is a potential candidate for anticancer drug. Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf application/pdf en http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf Salih, Ghassan Ali and Ahmad Raus, Raha and Shaban, Mustaffa Nameer and Abdullah, N. (2017) Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment. International Food Research Journal, 24 (Suppl.). pp. 281-286. ISSN 1985-4668 E-ISSN 2231-7546 http://www.ifrj.upm.edu.my/24%20(07)%202017%20supplementary/(4)%20R1.pdf |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
QD Chemistry QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) TP248.13 Biotechnology |
| spellingShingle |
QD Chemistry QK Botany RC0254 Neoplasms. Tumors. Oncology (including Cancer) TP248.13 Biotechnology Salih, Ghassan Ali Ahmad Raus, Raha Shaban, Mustaffa Nameer Abdullah, N. Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| description |
Breast cancer is considered as one of the most common cancers all over the world. A huge
effort has been made to create a safe and cost effective breast cancer treatment. All of these
features exist in the plants sources. In this study, the effect of local vegetable salad, Premna
serratifolia (Bebuas) against MCF-7 cells (human breast adenocarcinoma) was determined.
The optimum condition to extract breast cancer cytotoxic compound from the plant was
investigated and the exact cytotoxic compound was identified as well. To determine the plant
cytotoxicity effect against MCF-7 cells, MTT assay was used. Two important parameters in the
sonication extraction method which are duration of time and temperature were optimized by
carrying out a series of experiments which were designed by Face Centered Central Composite
Design (FCCCD). The extraction efficiency of each experiment was determined by measuring
the yield of extract and the half maximal inhibitory concentration (IC50) of the extract against
MCF-7 cells. The results obtained from the experiments were fitted to the second order
polynomial model to generate equation that was used to determine best extraction processing
condition. Based on the generated equation, the best sonication processing condition to extract
the cytotoxic compound is at 30o
C for 67 min. Analysis of variance (ANOVA) showed that the
duration of extraction time has great influence (p<0.05) on the yield of crude extract, while
both parameters, time and temperature significantly (p<0.05) affected the IC50 value of the
plant extract. Two purified compounds from the plant, acacetin and acacetin-7-O-glycoside
were identified to be responsible for the cytotoxicity activity. Conclusively, in terms of
pharmaceutical and medical field applications; the optimal extraction processing condition
obtained from this study ensures that the extraction of cytotoxic compound from the plant is
conducted at the low temperature and short duration of time. Further study on the identified
compounds is required as it is a potential candidate for anticancer drug. |
| format |
Article |
| author |
Salih, Ghassan Ali Ahmad Raus, Raha Shaban, Mustaffa Nameer Abdullah, N. |
| author_facet |
Salih, Ghassan Ali Ahmad Raus, Raha Shaban, Mustaffa Nameer Abdullah, N. |
| author_sort |
Salih, Ghassan Ali |
| title |
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| title_short |
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| title_full |
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| title_fullStr |
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| title_full_unstemmed |
Extraction and purification of cytotoxic compounds from Premna serratifolia L. (bebuas) for human breast cancer treatment |
| title_sort |
extraction and purification of cytotoxic compounds from premna serratifolia l. (bebuas) for human breast cancer treatment |
| publisher |
Faculty of Food Science & Technology, Universiti Putra Malaysia (UPM) |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/57590/ http://irep.iium.edu.my/57590/ http://irep.iium.edu.my/57590/1/57590_Extraction%20and%20purification%20of%20cytotoxic.pdf http://irep.iium.edu.my/57590/7/57590_Extraction%20and%20purification%20of%20cytotoxic%20compounds_scopus.pdf |
| first_indexed |
2023-09-18T21:21:25Z |
| last_indexed |
2023-09-18T21:21:25Z |
| _version_ |
1777411907118956544 |