Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient

Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either...

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Main Authors:	Othman, Rashidi, Mohd Zaifuddin, Fatimah Azzahra, Mohd Hassan, Norazian
Format:	Article
Language:	English English
Published:	Lifescience Global 2017
Subjects:	QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
Online Access:	http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf

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spelling	iium-576352018-01-16T07:45:00Z http://irep.iium.edu.my/57635/ Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either in natural or synthetic form but they are quite expensive and some of it not available in the market anymore. These problems strongly associated with the accuracy and reliability of High Performance Liquid Chromatography (HPLC) analysis data. Therefore, this study aimed to set up an analytical scheme of obtaining β-carotene standard from the leaves of Morinda citrifolia as one of the carotenoid standards for HPLC analysis. M. citrifolia has been selected due to its abundance throughout the year with tropical climate. The scheme via open column chromatography (OCC) established that the purity of β-carotene standard was 97% and the coefficient of correlation was 0.9923. However after 30 day storage period of time, the purity decreased to 95.46%. Although these had an effect on the carotenoid standard stability but it can be a reliable source of β-carotene standard for HPLC analysis as well as active pharmaceutical ingredient for cosmeceutical, nutraceutical, food and beverage industries. Lifescience Global 2017-07-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf application/pdf en http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf Othman, Rashidi and Mohd Zaifuddin, Fatimah Azzahra and Mohd Hassan, Norazian (2017) Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient. Journal of Pharmacy and Nutrition Sciences, 7 (3). pp. 95-99. ISSN 2223-3806 E-ISSN 1927-5951 http://www.lifescienceglobal.com/pms/index.php/jpans/article/view/4789
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc
spellingShingle	QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
description	Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either in natural or synthetic form but they are quite expensive and some of it not available in the market anymore. These problems strongly associated with the accuracy and reliability of High Performance Liquid Chromatography (HPLC) analysis data. Therefore, this study aimed to set up an analytical scheme of obtaining β-carotene standard from the leaves of Morinda citrifolia as one of the carotenoid standards for HPLC analysis. M. citrifolia has been selected due to its abundance throughout the year with tropical climate. The scheme via open column chromatography (OCC) established that the purity of β-carotene standard was 97% and the coefficient of correlation was 0.9923. However after 30 day storage period of time, the purity decreased to 95.46%. Although these had an effect on the carotenoid standard stability but it can be a reliable source of β-carotene standard for HPLC analysis as well as active pharmaceutical ingredient for cosmeceutical, nutraceutical, food and beverage industries.
format	Article
author	Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian
author_facet	Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian
author_sort	Othman, Rashidi
title	Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
title_short	Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
title_full	Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
title_fullStr	Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
title_full_unstemmed	Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
title_sort	isolation and purification of β-carotene from morinda citrifolia as hplc standard and active pharmaceutical ingredient
publisher	Lifescience Global
publishDate	2017
url	http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf
first_indexed	2023-09-18T21:21:29Z
last_indexed	2023-09-18T21:21:29Z
_version_	1777411911261880320

Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient

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