A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics

Arabidopsis thaliana (A. thaliana) is a small weed which is related to the cabbage and mustard family. This flowering plant has been used widely as a model plant in genetics and molecular biology research since it is the first plant the full sequenced genome. Thus, A. thaliana literature provides pl...

Full description

Bibliographic Details
Main Authors:	Mohamad Puad, Noor Illi, Abd-Karim, Khairiah, Mavituna, Ferda
Format:	Article
Language:	English English
Published:	Penerbit UTM Pres 2017
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/1/Published%20Manuscript.pdf http://irep.iium.edu.my/57696/7/57696_A%20model%20for%20Arabidopsis%20thaliana%20cell_SCOPUS.pdf

id	iium-57696
recordtype	eprints
spelling	iium-576962018-03-28T01:50:45Z http://irep.iium.edu.my/57696/ A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda T Technology (General) Arabidopsis thaliana (A. thaliana) is a small weed which is related to the cabbage and mustard family. This flowering plant has been used widely as a model plant in genetics and molecular biology research since it is the first plant the full sequenced genome. Thus, A. thaliana literature provides plentiful information from genomics and transcriptomics point of view. However, there is still a lack of physiological information regarding its cell suspension cultures which can be powerful research tools in Plant Biotechnology and especially in Plant Systems and Synthetic Biology. In this study, cell growth and sugar uptake of A. thaliana Col ecotype grown in the continuous dark condition were modelled using the modified Monod and Michelis-menten equations. The model included sucrose hydrolysis by the cell-wall invertase enzyme into hexoses (glucose and fructose) and consumption of these hexoses at different rates to support cell growth. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells. This model can also be applied for larger scale of growth with extended expressions for oxygen uptake rate, carbon dioxide production rate etc. Penerbit UTM Pres 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57696/1/Published%20Manuscript.pdf application/pdf en http://irep.iium.edu.my/57696/7/57696_A%20model%20for%20Arabidopsis%20thaliana%20cell_SCOPUS.pdf Mohamad Puad, Noor Illi and Abd-Karim, Khairiah and Mavituna, Ferda (2017) A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics. Jurnal Teknologi, 79 (5-3). pp. 81-87. ISSN 0127-9696 E-ISSN 2180-3722 hhttp://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11331/6197 10.11113/jt.v79.11331
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General)
spellingShingle	T Technology (General) Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
description	Arabidopsis thaliana (A. thaliana) is a small weed which is related to the cabbage and mustard family. This flowering plant has been used widely as a model plant in genetics and molecular biology research since it is the first plant the full sequenced genome. Thus, A. thaliana literature provides plentiful information from genomics and transcriptomics point of view. However, there is still a lack of physiological information regarding its cell suspension cultures which can be powerful research tools in Plant Biotechnology and especially in Plant Systems and Synthetic Biology. In this study, cell growth and sugar uptake of A. thaliana Col ecotype grown in the continuous dark condition were modelled using the modified Monod and Michelis-menten equations. The model included sucrose hydrolysis by the cell-wall invertase enzyme into hexoses (glucose and fructose) and consumption of these hexoses at different rates to support cell growth. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells. This model can also be applied for larger scale of growth with extended expressions for oxygen uptake rate, carbon dioxide production rate etc.
format	Article
author	Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda
author_facet	Mohamad Puad, Noor Illi Abd-Karim, Khairiah Mavituna, Ferda
author_sort	Mohamad Puad, Noor Illi
title	A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_short	A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_full	A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_fullStr	A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_full_unstemmed	A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics
title_sort	model for arabidopsis thaliana cell suspension growth and sugar uptake kinetics
publisher	Penerbit UTM Pres
publishDate	2017
url	http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/ http://irep.iium.edu.my/57696/1/Published%20Manuscript.pdf http://irep.iium.edu.my/57696/7/57696_A%20model%20for%20Arabidopsis%20thaliana%20cell_SCOPUS.pdf
first_indexed	2023-09-18T21:21:35Z
last_indexed	2023-09-18T21:21:35Z
_version_	1777411917329989632

A model for Arabidopsis thaliana cell suspension growth and sugar uptake kinetics

Similar Items