Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design

A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, am...

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Main Authors: Abdul Wahid, Manisya Zauri, Salleh, Madihah, Yusof, Faridah, Abdul Karim, Mohamed Ismail, Alam, Md Zahangir
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
Online Access:http://irep.iium.edu.my/5775/
http://irep.iium.edu.my/5775/
http://irep.iium.edu.my/5775/1/Paper-manysha.pdf
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spelling iium-57752017-08-10T02:40:53Z http://irep.iium.edu.my/5775/ Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design Abdul Wahid, Manisya Zauri Salleh, Madihah Yusof, Faridah Abdul Karim, Mohamed Ismail Alam, Md Zahangir TP248.13 Biotechnology A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p=0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p=0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of Pmodel>F= 0.0008 and R2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity. Academic Journals 2011-08-22 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5775/1/Paper-manysha.pdf Abdul Wahid, Manisya Zauri and Salleh, Madihah and Yusof, Faridah and Abdul Karim, Mohamed Ismail and Alam, Md Zahangir (2011) Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design. African Journal of Biotechnology, 10 (46). pp. 9402-9409. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/22Aug/Wahid%20et%20al.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Abdul Wahid, Manisya Zauri
Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohamed Ismail
Alam, Md Zahangir
Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
description A study was conducted to screen parameters affecting the production of endoglucanase by Trichoderma reesei RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p=0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p=0.023) have been recognized as significant factors affecting endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by T. reesei RUT C-30. The model established from the ANOVA analysis have a significant value of Pmodel>F= 0.0008 and R2 of 0.9132. The preoptimized media showed 2.6 fold increased of endoglucanase activity.
format Article
author Abdul Wahid, Manisya Zauri
Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohamed Ismail
Alam, Md Zahangir
author_facet Abdul Wahid, Manisya Zauri
Salleh, Madihah
Yusof, Faridah
Abdul Karim, Mohamed Ismail
Alam, Md Zahangir
author_sort Abdul Wahid, Manisya Zauri
title Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_short Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_full Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_fullStr Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_full_unstemmed Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
title_sort factors affecting endoglucanase production by trichoderma reesei rut c-30 from solid state fermentation of oil palm empty fruit bunches using plackett-burman design
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/5775/
http://irep.iium.edu.my/5775/
http://irep.iium.edu.my/5775/1/Paper-manysha.pdf
first_indexed 2023-09-18T20:14:33Z
last_indexed 2023-09-18T20:14:33Z
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