Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation

Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation

INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of t...

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Main Authors: Zulkifly, Ahmad Hafiz, Mohd Jan, Nurul Hafiza, Ibrahim, Mohd Zulfadzli, Aziz, Aziah
Format: Conference or Workshop Item
Language:English
Published: Malaysian Orthopaedic Association 2017
Subjects:
RD701 Orthopedics
Online Access:http://irep.iium.edu.my/57875/
http://irep.iium.edu.my/57875/
http://irep.iium.edu.my/57875/19/57875.pdf
id iium-57875
recordtype eprints
spelling iium-578752017-08-04T08:04:38Z http://irep.iium.edu.my/57875/ Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation Zulkifly, Ahmad Hafiz Mohd Jan, Nurul Hafiza Ibrahim, Mohd Zulfadzli Aziz, Aziah RD701 Orthopedics INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of the study is to evaluate the outcome treatment of osteomyelitis in rabbit femur with impregnated Hydroxyappatite Gentamicin. Evaluation at stipulated durations using microCT Skyscan 1176 system. MATERIALS & METHODS: Osteomyelitis is created by inoculation of Staphylococcus aureus ATCC 25923 into rabbit’s distal femur. The rabbit were divided with 3, 6, 12, 26 week assessments. The response of the biomaterials (hydroxyapatite) impregnated with gentamicin against the infection were evaluated through micro-computed topography using Skyscan 1176 system.The bone analysis data were obtained by the software provided by Skyscan 1176 system. The distal femoral shaft were scanned using A1 1mm filter with 18µm pixel resolutions. Bacterial study of culture and sensitivity, coagulase test, catalase test and gram stain were done to determine the treatment is effective. RESULTS: The data of bone analysis were for tissue volume (TV), bone surface (BS), bone volume (BV), and trabecular thickness (Tb.Th) in all groups were illustrated at Table 1. The bone healing noted in the treated group. The results showed no bacterial were grow in the agar and all test were negative at 26 weeks interval. Table 1 showing the bone analysis through Skyscan1176 system. Week & parameter Tissue volume (mm3) Bone volume (mm3) Bone surface(mm3) Trabecular thickness (mm) 3 W SHAM 156.692 66.962 399.351 0.045 3W post treatment 168.051 84.762 500.962 0.405 6W post treatment 409.204 104.952 1001.135 0.456 12 W treatment 590.716 113.485 1520.599 0.549 26w post treatment 635.955 124.958 1558.096 0.723 DISCUSSIONS: Osteomyelitic changes were noted in all rabbit after inoculation of bacteria at week three and six. The micro-computed topographic analysis at 3, 6, 12 and 26 weeks of interval, showed increase bone parameters in treated femur. The bone volume (BV) was increased with value 124.96 mm3 compared to sham group (no treatment given) with bone volume 66.96 mm3. The density of biomaterials was decreased with duration of studies. This data showed that micro-computed topography studies is capable to provide information of bone microstructures in rapid and non-destructive way. The findings showed that impregnated biomaterials with gentamicin have potential to be used in osteomyelitis infection. CONCLUSION: Computed topography results showed at 26 weeks of treatment, the impregnated biomaterials have a potential in treatment of osteomyelitis. Malaysian Orthopaedic Association 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/57875/19/57875.pdf Zulkifly, Ahmad Hafiz and Mohd Jan, Nurul Hafiza and Ibrahim, Mohd Zulfadzli and Aziz, Aziah (2017) Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation. In: 50th Anniversary Golden Jubilee of Malaysian Orthopaedic Association & 47th Annual Scientific Meeting / Annual General Meeting 2017, 19th-21st May 2017, Kuala Lumpur, Malaysia. (Unpublished) http://moa-home.com/moa2017/posterslist1.html
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RD701 Orthopedics
spellingShingle RD701 Orthopedics
Zulkifly, Ahmad Hafiz
Mohd Jan, Nurul Hafiza
Ibrahim, Mohd Zulfadzli
Aziz, Aziah
Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
description INTRODUCTION: Treatment of osteomyelitis is still a major challenge in orthopaedic field. New Zealand White rabbit is acceptable experimental model described to develop effective local delivery of antibiotics osteomyelitis treatment as it can mimics the disease process in human. The objective of the study is to evaluate the outcome treatment of osteomyelitis in rabbit femur with impregnated Hydroxyappatite Gentamicin. Evaluation at stipulated durations using microCT Skyscan 1176 system. MATERIALS & METHODS: Osteomyelitis is created by inoculation of Staphylococcus aureus ATCC 25923 into rabbit’s distal femur. The rabbit were divided with 3, 6, 12, 26 week assessments. The response of the biomaterials (hydroxyapatite) impregnated with gentamicin against the infection were evaluated through micro-computed topography using Skyscan 1176 system.The bone analysis data were obtained by the software provided by Skyscan 1176 system. The distal femoral shaft were scanned using A1 1mm filter with 18µm pixel resolutions. Bacterial study of culture and sensitivity, coagulase test, catalase test and gram stain were done to determine the treatment is effective. RESULTS: The data of bone analysis were for tissue volume (TV), bone surface (BS), bone volume (BV), and trabecular thickness (Tb.Th) in all groups were illustrated at Table 1. The bone healing noted in the treated group. The results showed no bacterial were grow in the agar and all test were negative at 26 weeks interval. Table 1 showing the bone analysis through Skyscan1176 system. Week & parameter Tissue volume (mm3) Bone volume (mm3) Bone surface(mm3) Trabecular thickness (mm) 3 W SHAM 156.692 66.962 399.351 0.045 3W post treatment 168.051 84.762 500.962 0.405 6W post treatment 409.204 104.952 1001.135 0.456 12 W treatment 590.716 113.485 1520.599 0.549 26w post treatment 635.955 124.958 1558.096 0.723 DISCUSSIONS: Osteomyelitic changes were noted in all rabbit after inoculation of bacteria at week three and six. The micro-computed topographic analysis at 3, 6, 12 and 26 weeks of interval, showed increase bone parameters in treated femur. The bone volume (BV) was increased with value 124.96 mm3 compared to sham group (no treatment given) with bone volume 66.96 mm3. The density of biomaterials was decreased with duration of studies. This data showed that micro-computed topography studies is capable to provide information of bone microstructures in rapid and non-destructive way. The findings showed that impregnated biomaterials with gentamicin have potential to be used in osteomyelitis infection. CONCLUSION: Computed topography results showed at 26 weeks of treatment, the impregnated biomaterials have a potential in treatment of osteomyelitis.
format Conference or Workshop Item
author Zulkifly, Ahmad Hafiz
Mohd Jan, Nurul Hafiza
Ibrahim, Mohd Zulfadzli
Aziz, Aziah
author_facet Zulkifly, Ahmad Hafiz
Mohd Jan, Nurul Hafiza
Ibrahim, Mohd Zulfadzli
Aziz, Aziah
author_sort Zulkifly, Ahmad Hafiz
title Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
title_short Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
title_full Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
title_fullStr Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
title_full_unstemmed Treatment of osteomyelitis in New Zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
title_sort treatment of osteomyelitis in new zealand white rabbit using impregnated biomaterials with gentamicin: in vivo study outcome of bacterial study and micro-computed topography evaluation
publisher Malaysian Orthopaedic Association
publishDate 2017
url http://irep.iium.edu.my/57875/
http://irep.iium.edu.my/57875/
http://irep.iium.edu.my/57875/19/57875.pdf
first_indexed 2023-09-18T21:21:50Z
last_indexed 2023-09-18T21:21:50Z
_version_ 1777411933166632960

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