Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Abdul Hamid, Azzmer Azzar
Format:	Article
Language:	English English
Published:	Open science Publisher 2017
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf

id	iium-57890
recordtype	eprints
spelling	iium-578902017-09-20T06:51:34Z http://irep.iium.edu.my/57890/ Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar QR Microbiology E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures (TMP) through vacuum suction of 0.1 to 0.7 bar were applied to the plasmid sample, and the corresponding flow rates and fluxes for both filters were investigated. Even though, these two filters showed a slight different in the flux, a marked different in DNA transmission were observed. DNA transmission were generally higher with cellulose generated filters, whereby DNA transmission by Ultracel filter was at44.3, 63.9, 74.1, 55.6 and 54.5% at TMP of 0.1, 0.18, 0.39, 0.6 and 0.7 bar, respectively. Meanwhile, for PES filters the DNA transmission was at 22.3, 38.1, 39.1, 38.0 and 37.5% at TMP of 0.1, 0.3, 0.4, 0.6 and 0.7 bar, respectively. With the cellulose generated filter, DNA transmission reached an optimum (~70%) at about 0.6 bar after which the transmission depleted at higher TMP of 0.7 bar. Throughout all of the TMP, DNA transmissions observed were generally lower with PES filter. The properties of the filter material could have contributed to the differences in DNA permeation Open science Publisher 2017-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf application/pdf en http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf Tengku Abdul Hamid, Tengku Haziyamin and Abdul Hamid, Azzmer Azzar (2017) Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters. Journal of Applied Pharmaceutical Science, 7 (7). pp. 46-50. ISSN 2231-3354 http://www.japsonline.com/admin/php/uploads/2327_pdf.pdf 10.7324/JAPS.2017.70707
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
description	E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures (TMP) through vacuum suction of 0.1 to 0.7 bar were applied to the plasmid sample, and the corresponding flow rates and fluxes for both filters were investigated. Even though, these two filters showed a slight different in the flux, a marked different in DNA transmission were observed. DNA transmission were generally higher with cellulose generated filters, whereby DNA transmission by Ultracel filter was at44.3, 63.9, 74.1, 55.6 and 54.5% at TMP of 0.1, 0.18, 0.39, 0.6 and 0.7 bar, respectively. Meanwhile, for PES filters the DNA transmission was at 22.3, 38.1, 39.1, 38.0 and 37.5% at TMP of 0.1, 0.3, 0.4, 0.6 and 0.7 bar, respectively. With the cellulose generated filter, DNA transmission reached an optimum (~70%) at about 0.6 bar after which the transmission depleted at higher TMP of 0.7 bar. Throughout all of the TMP, DNA transmissions observed were generally lower with PES filter. The properties of the filter material could have contributed to the differences in DNA permeation
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Abdul Hamid, Azzmer Azzar
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
title_short	Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
title_full	Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
title_fullStr	Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
title_full_unstemmed	Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
title_sort	investigation on plasmid dna separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
publisher	Open science Publisher
publishDate	2017
url	http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf
first_indexed	2023-09-18T21:21:51Z
last_indexed	2023-09-18T21:21:51Z
_version_	1777411934226743296

Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

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