Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide

Background: Exposure to cyclophosphamide (CPA) during chemotherapy results in over-production of reactive oxygen species and oxidative stress thus causing sperm defects following disturbance to the genetic material of male germ cells. The goal of this study is to assess the potential effects of Nige...

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Main Authors:	Abdul Rahman, Suzanah, Abdul Samat, Nadia Hanis, Ahmad, Nur Amalina, Abdullah, 'Afif Raihan, Mohd Zahri, Syazana, Kamarzaman, Saheera
Format:	Article
Language:	English English English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2017
Subjects:	RG Gynecology and obstetrics RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/58232/ http://irep.iium.edu.my/58232/ http://irep.iium.edu.my/58232/1/IMJM-Vol-16-p099-106.pdf http://irep.iium.edu.my/58232/7/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_SCOPUS.pdf http://irep.iium.edu.my/58232/8/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_WOS.pdf

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spelling	iium-582322018-01-11T02:45:21Z http://irep.iium.edu.my/58232/ Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohd Zahri, Syazana Kamarzaman, Saheera RG Gynecology and obstetrics RM300 Drugs and their action Background: Exposure to cyclophosphamide (CPA) during chemotherapy results in over-production of reactive oxygen species and oxidative stress thus causing sperm defects following disturbance to the genetic material of male germ cells. The goal of this study is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality subsequent to paternal exposure to a toxic level of CPA. Methods: Thirty-six male ICR mice were divided into six groups; (I) Vehicle-treated control (normal saline), (II) CPA-only, (III) TQ-only, (IV) NSE-only, (V) CPA followed by TQ and (VI) CPA followed by NSE. Treatment with 200mg/kg CPA and 10mg/kg of both NSE and TQ were given by intraperitoneal injection. Animals were sacrificed at day 33 by cervical dislocation and sperm from caudal epidydymis were taken for analysis and in vitro fertilisation (IVF) with eggs from untreated female. Fertilization rates and embryo development were monitored for 5 days. Results: TQ and NSE supplementation to CPA-exposed male mice have no significant effect on the total number of sperm when compared to CPA-only exposed mice. NSE and TQ supplementation have been shown to have significant effects on the percentage of motile sperm as well as the number of abnormal sperm. Four types of sperm abnormalities were observed and they included folded sperm, amorphous, banana-like and the head lacking of the usual hook. The embryo quality showed a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice. Conclusion: Both NSE and TQ exhibited chemopreventive potential against the toxic alkylating effects of CPA on the reproductive capacity and fertility of mice. Kulliyyah of Medicine, International Islamic University Malaysia 2017-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58232/1/IMJM-Vol-16-p099-106.pdf application/pdf en http://irep.iium.edu.my/58232/7/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/58232/8/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_WOS.pdf Abdul Rahman, Suzanah and Abdul Samat, Nadia Hanis and Ahmad, Nur Amalina and Abdullah, 'Afif Raihan and Mohd Zahri, Syazana and Kamarzaman, Saheera (2017) Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide. The International Medical Journal of Malaysia, 16 (1). pp. 99-106. ISSN 1823-4631 http://journals.iium.edu.my/imjm/index.php/eimj/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RG Gynecology and obstetrics RM300 Drugs and their action
spellingShingle	RG Gynecology and obstetrics RM300 Drugs and their action Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohd Zahri, Syazana Kamarzaman, Saheera Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
description	Background: Exposure to cyclophosphamide (CPA) during chemotherapy results in over-production of reactive oxygen species and oxidative stress thus causing sperm defects following disturbance to the genetic material of male germ cells. The goal of this study is to assess the potential effects of Nigella sativa extract (NSE) and thymoquinone (TQ) on sperm and embryo quality subsequent to paternal exposure to a toxic level of CPA. Methods: Thirty-six male ICR mice were divided into six groups; (I) Vehicle-treated control (normal saline), (II) CPA-only, (III) TQ-only, (IV) NSE-only, (V) CPA followed by TQ and (VI) CPA followed by NSE. Treatment with 200mg/kg CPA and 10mg/kg of both NSE and TQ were given by intraperitoneal injection. Animals were sacrificed at day 33 by cervical dislocation and sperm from caudal epidydymis were taken for analysis and in vitro fertilisation (IVF) with eggs from untreated female. Fertilization rates and embryo development were monitored for 5 days. Results: TQ and NSE supplementation to CPA-exposed male mice have no significant effect on the total number of sperm when compared to CPA-only exposed mice. NSE and TQ supplementation have been shown to have significant effects on the percentage of motile sperm as well as the number of abnormal sperm. Four types of sperm abnormalities were observed and they included folded sperm, amorphous, banana-like and the head lacking of the usual hook. The embryo quality showed a significant improvement by the supplementation of TQ and NSE to CPA-exposed male mice. Conclusion: Both NSE and TQ exhibited chemopreventive potential against the toxic alkylating effects of CPA on the reproductive capacity and fertility of mice.
format	Article
author	Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohd Zahri, Syazana Kamarzaman, Saheera
author_facet	Abdul Rahman, Suzanah Abdul Samat, Nadia Hanis Ahmad, Nur Amalina Abdullah, 'Afif Raihan Mohd Zahri, Syazana Kamarzaman, Saheera
author_sort	Abdul Rahman, Suzanah
title	Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
title_short	Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
title_full	Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
title_fullStr	Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
title_full_unstemmed	Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
title_sort	investigating the potential of nigella sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/58232/ http://irep.iium.edu.my/58232/ http://irep.iium.edu.my/58232/1/IMJM-Vol-16-p099-106.pdf http://irep.iium.edu.my/58232/7/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_SCOPUS.pdf http://irep.iium.edu.my/58232/8/58232-Investigating%20the%20Potential%20of%20Nigella%20Sativa_WOS.pdf
first_indexed	2023-09-18T21:22:19Z
last_indexed	2023-09-18T21:22:19Z
_version_	1777411964094382080

Investigating the potential of Nigella Sativa and thymoquinone in salvaging the embryo from effects of toxic paternal exposure to cyclophosphamide

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